Method and device for pipetting control of plate inoculation instrument and pipette thereof
A control method and pipette technology, which are applied in chemical instruments and methods, biological material sampling methods, biochemical instruments, etc., can solve the problem of inaccurate control, affecting the uniformity of liquid sample inoculation, and affecting the separation of single colonies of inoculated streaks. effect and other issues, to achieve the effect of proper and accurate control of the downward movement distance and improved uniformity
Active Publication Date: 2022-08-05
AUTOBIO LABTEC INSTR CO LTD
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Problems solved by technology
[0003] However, in the actual inoculation process, the moving distance driven by the pipette to the agar surface often has the problem of inaccurate control, which ultimately affects the uniformity of inoculation of the liquid sample on the agar surface, thereby affecting the effect of inoculation streaking and single colony isolation
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[0044] The main components of the plate inoculation instrument include a plate structure for carrying agar, a pipette directly above the plate structure, a driving device for controlling the up and down movement of the pipette, and so on. In the process of transferring and inoculating liquid samples, the plate inoculator needs to clamp the micropipettes carrying the liquid samples on the pipette, and then drive the pipettes to move down to the bottom of the micropipettes through the driving device. Adheres to the agar surface, so that the liquid sample is released from the bottom end of the micropipette to the agar surface.
[0045] In the process of moving down the micropipette with the pipette, generally based on the relative distance between the pipette and the plate structure, the pipette is driven to move down a certain height so that the bottom end of the micropipette is just close to the agar surface. However, in practical application, each micropipette has a difference...
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The invention discloses a pipetting control method for a pipette of a plate inoculation apparatus, which includes collecting detection images including the bottom end of a micropipette clamped by the pipette and a reference point mark; and performing image recognition on the detection images. , determine the vertical height difference between the bottom end of the micropipette and the reference point mark; according to the vertical height difference, and the predetermined fixed distance of the reference point mark relative to the upper surface of the plate structure used to carry the agar, determine the micropipette The bottom end of the tip needs to move down the distance, and control the pipette to move the bottom end of the micropipette down to the surface of the agar according to the distance. In the present application, the machine vision technology is used to make the control of the moving distance of the micropipette more suitable and accurate, which improves the uniformity of the liquid sample inoculation on the agar surface and the effect of subsequent colony culture. The present application also provides a pipetting control device for a pipette of a plate inoculation device, and a plate inoculation device, which have the above beneficial effects.
Description
technical field [0001] The invention relates to the field of bacterial isolation and culture inoculated by a plate inoculation instrument, in particular to a liquid transfer control method and device of a pipette in a plate inoculation instrument, and a plate inoculation instrument. Background technique [0002] The plate inoculation instrument is mainly used to inoculate liquid samples (such as sputum, urine, blood, cerebrospinal fluid, pleural and ascites, etc.) to isolate single colonies of pathogenic bacteria, which is conducive to assisting clinical treatment and colony research. In the process of inoculating liquid samples in the plate inoculation instrument, the liquid samples are sucked through a disposable hydrophobic micropipette (TIP for short), and the micropipette is moved to the agar surface in the plate by a pipette for inoculation. [0003] However, in the actual inoculation process, the moving distance that the pipette drives the micropipette to move to the ...
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IPC IPC(8): B01L3/02C12M1/26
CPCB01L3/021C12M33/04
Inventor 侯剑平王超马海伟杨东方赵万里王驰吴胜军范海滨马宝彬刘聪
Owner AUTOBIO LABTEC INSTR CO LTD