Flat plate inoculation instrument, pipetting control method and device of pipettor of flat plate inoculation instrument
A control method and pipette technology, which are applied in chemical instruments and methods, biological material sampling methods, biochemical instruments, etc., can solve the problem of inaccurate control, affecting the uniformity of liquid sample inoculation, and affecting the separation of single colonies of inoculated streaks. effects, etc.
Active Publication Date: 2021-07-06
AUTOBIO LABTEC INSTR CO LTD
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Problems solved by technology
[0003] However, in the actual inoculation process, the moving distance driven by the pipette to the agar surface often has the problem of inaccurate control, which ultimately affects the uniformity of inoculation of the liquid sample on the agar surface, thereby affecting the effect of inoculation streaking and single colony isolation
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[0044] The main components of the plate inoculator include a plate structure for carrying agar, a pipette located directly above the plate structure, a driving device for controlling the pipette to move up and down, and so on. In the process of transferring and inoculating the liquid sample, the plate inoculator needs to clamp the micropipette carrying the liquid sample on the pipette, and then drive the pipette to move down to the bottom of the micropipette through the driving device Fits snugly onto the agar surface so that the liquid sample is released from the bottom end of the micropipette onto the agar surface.
[0045] During the downward movement of the pipette carrying the micropipette, the pipette is generally driven down to a specific height based on the relative distance between the pipette and the plate structure so that the bottom of the micropipette is just close to the agar surface. However, in the actual application process, each micropipette has a difference ...
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The invention discloses a pipetting control method of a pipettor of a flat plate inoculation instrument. The method comprises the following steps: collecting a detection image including the bottom end of a micro-suction head clamped by the pipettor and a datum point identifier; performing image recognition on the detection image, and determining a vertical height difference between the bottom end of the micro suction head and the reference point identifier; and according to the vertical height difference and the fixed distance of a predetermined reference point mark relative to the upper surface of the flat plate structure used for bearing the agar, determining the downward moving distance of the bottom end of the micro suction head needing to move downwards, and according to the downward moving distance, controlling the pipettor to drive the bottom end of the micro suction head to move downwards to the surface of the agar. The machine vision technology is utilized, so that the downward moving distance of the micro suction head is controlled more appropriately and accurately, the inoculation uniformity of a liquid sample on the agar surface is improved, and the subsequent bacterial colony culture effect is improved. The invention further provides a pipetting control device of the pipettor of the flat plate inoculation instrument and the flat plate inoculation instrument which have the beneficial effects.
Description
technical field [0001] The invention relates to the field of isolation and cultivation of bacteria inoculated with a plate inoculator, in particular to a pipetting control method and device for a pipette in a plate inoculator and a plate inoculator. Background technique [0002] The plate inoculator is mainly used to inoculate liquid samples (such as sputum, urine, blood, cerebrospinal fluid, ascites, etc.) to isolate single colonies of pathogenic bacteria, which is beneficial to assist clinical treatment and colony research. In the process of inoculating liquid samples in the plate inoculator, liquid samples are drawn through a disposable hydrophobic micropipette (TIP for short), and the micropipette is clamped by a pipette to move to the agar surface in the plate for inoculation. [0003] However, in the actual inoculation process, the moving distance driven by the pipette to the agar surface often has the problem of inaccurate control, which ultimately affects the uniform...
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IPC IPC(8): B01L3/02C12M1/26
CPCB01L3/021C12M33/04
Inventor 侯剑平王超马海伟杨东方赵万里王驰吴胜军范海滨马宝彬刘聪
Owner AUTOBIO LABTEC INSTR CO LTD