Universal platelet preparation prepared from umbilical cord-derived mesenchymal stem cells and method

A technology for stem cells and platelets, which can be used in cell culture active agents, biochemical equipment and methods, animal cells, etc., and can solve the problems of inconvenient preparation and storage, poor universality of platelets, etc.

Active Publication Date: 2021-07-06
个体化细胞治疗技术国家地方联合工程实验室(深圳)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the above technical problems, this application discloses a general-purpose platelet preparation and method prepared from umbilical cor

Method used

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  • Universal platelet preparation prepared from umbilical cord-derived mesenchymal stem cells and method
  • Universal platelet preparation prepared from umbilical cord-derived mesenchymal stem cells and method
  • Universal platelet preparation prepared from umbilical cord-derived mesenchymal stem cells and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Preparation of Umbilical Cord Mesenchymal Stem Cells

[0048] 1.1 With informed consent, fresh umbilical cords were obtained for stripping to obtain Wharton's jelly, and after the Wharton's jelly was cut into pieces, primary cell culture was carried out in a serum-free culture system by using the adherent method. The serum-free culture system For: DMEM / F11 + commercially available MSCs culture factors.

[0049] 1.2 The primary umbilical cord mesenchymal stem cells are amplified, the cell generation is between P3-P5, and they are used to induce transformation to adipogenicity. The day before induction (Day-1), the cell fusion degree should be greater than 85% and Cultured at 37°C, 5% CO 2 in the incubator.

[0050] 2. Induction phase

[0051] 2.1 Adipogenic induction stage

[0052] 2.1.1 When adipogenic induction is started (Day0), the serum-free culture system is completely replaced with the adipogenic induction medium, and the cells are cultured for 10 days. The...

Embodiment 2

[0063] 1. Preparation of Umbilical Cord Mesenchymal Stem Cells

[0064] 1.1 With informed consent, fresh umbilical cords were obtained for stripping to obtain Wharton's jelly, and after the Wharton's jelly was cut into pieces, primary cell culture was carried out in a serum-free culture system by using the adherent method. The serum-free culture system For: DMEM / F11 + commercially available MSCs culture factors.

[0065] 1.2 The primary umbilical cord mesenchymal stem cells are amplified, the cell generation is between P3-P5, and they are used to induce transformation to adipogenicity. The day before induction (Day-1), the cell fusion degree should be greater than 85% and Cultured at 37°C, 5% CO 2 in the incubator.

[0066] 2. Induction phase

[0067] 2.1 Adipogenic induction stage

[0068] 2.1.1 When adipogenic induction is started (Day0), the serum-free culture system is completely replaced with the adipogenic induction medium, and the cells are cultured for 16 days. The...

Embodiment 3

[0079] 1. Preparation of Umbilical Cord Mesenchymal Stem Cells

[0080] 1.1 With informed consent, fresh umbilical cords were obtained for stripping to obtain Wharton's jelly, and after the Wharton's jelly was cut into pieces, primary cell culture was carried out in a serum-free culture system by using the adherent method. The serum-free culture system For: DMEM / F11 + commercially available MSCs culture factors.

[0081] 1.2 The primary umbilical cord mesenchymal stem cells are amplified, the cell generation is between P3-P5, and they are used to induce transformation to adipogenicity. The day before induction (Day-1), the cell fusion degree should be greater than 85% and Cultured at 37°C, 5% CO 2 in the incubator.

[0082] 2 induction phase

[0083] 2.1 Adipogenic induction stage

[0084] 2.1.1 When adipogenic induction is started (Day0), the serum-free culture system is completely replaced with the adipogenic induction medium, and the cells are cultured for 13 days. The ...

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Abstract

The invention discloses a universal platelet preparation prepared from umbilical cord-derived mesenchymal stem cells and a method. The method comprises the following steps: providing the umbilical cord-derived mesenchymal stem cells; performing multi-stage induction treatment on the umbilical cord-derived mesenchymal stem cells to prepare a platelet preparation; wherein the platelets are universal platelets with low immunogenicity. The preparation method is convenient to operate, and the preparation is convenient to store and basically can be taken at any time. In addition, the platelet preparation prepared by the method has good universality, the problem of ineffective infusion caused by immune factors can be effectively reduced, and the infusion efficiency can be improved.

Description

technical field [0001] The invention relates to the technical field of biotechnology, in particular to a universal platelet preparation prepared from umbilical cord-derived mesenchymal stem cells and a method thereof. Background technique [0002] Platelet transfusion (PLT) therapy is clinically mainly used to prevent and treat bleeding caused by decreased platelet count or dysfunction, so as to restore and maintain the body's hemostatic and coagulation functions. [0003] In the prior art, platelet preparations for clinical transfusion rely on blood donation volunteers to obtain platelets through blood cell apheresis machines. The shelf life of platelet preparations obtained by apheresis is very short, generally 4 to 5 days. So far, the industry has not developed a method for long-term storage of platelets with therapeutic effects. Therefore, considering the time required for inspection and transportation, the in vitro storage time of platelet preparations is generally abou...

Claims

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Application Information

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IPC IPC(8): C12N5/078C12N5/0775
CPCC12N5/0644C12N2506/1369C12N2500/25C12N2500/32C12N2500/40C12N2501/39C12N2501/999C12N2501/145C12N2501/998C12N2500/44C12N2501/125C12N2501/2303C12N2501/2306C12N2501/2311Y02A50/30
Inventor 刘沐芸廖延傅泽钦曾桂芳伍世铎李端端梁晓
Owner 个体化细胞治疗技术国家地方联合工程实验室(深圳)
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