Dipterex electrogenerated chemiluminescence molecular imprinting sensor as well as preparation method and application thereof

A technology of molecular imprinting and electrochemistry, applied in the field of detection, can solve the problems of low detection range, high detection limit, complicated method operation, etc., and achieve the effect of enhanced luminescence effect and high selectivity

Pending Publication Date: 2021-07-09
ANALYSIS & TESTING CENT CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the above methods have their own advantages, there are still great shortcomings. Although the liquid chromatography-mass spectrometry method has high detection precision, the instrument is relatively expensive, and the detection cost is very high; gas chromatography requires multiple extractions and conversions, and the method operation Extremely complex and introduce large errors
Flow injection chemiluminescence method has low detection range, high detection limit and poor selectivity

Method used

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  • Dipterex electrogenerated chemiluminescence molecular imprinting sensor as well as preparation method and application thereof
  • Dipterex electrogenerated chemiluminescence molecular imprinting sensor as well as preparation method and application thereof
  • Dipterex electrogenerated chemiluminescence molecular imprinting sensor as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0043] This embodiment provides a preparation method of trichlorfon electrochemiluminescent molecularly imprinted sensor, comprising the following steps:

[0044] (1) Preparation of molecularly imprinted sensors:

[0045] Grind and polish the gold electrode on the suede with 0.3 μm α-alumina until the surface is mirror-like, then put the gold electrode into secondary water and ultrasonically vibrate for 3 minutes to clean the surface;

[0046] Take 3mL 1.0×10 -3 mol / L o-phenylenediamine solution, 1mL 1.0×10 -3 mol / L trichlorfon solution, made into polyfilm bottom solution;

[0047] Put the cleaned gold electrode into the poly-film bottom solution, electropolymerize by cyclic voltammetry, the potential range is 0.8-0V, the scan rate (can rates) is 0.05V / s, and the number of sweep segments (sweep segments) is 15-30. to obtain electropolymers;

[0048] The electropolymer was rinsed with secondary water and allowed to stand for 1 minute, then eluted with 0.10mol / L NaOH-absolut...

Embodiment 2

[0053] This embodiment provides a preparation method of trichlorfon electrochemiluminescent molecularly imprinted sensor, comprising the following steps:

[0054] (1) Preparation of molecularly imprinted sensors:

[0055] Grind and polish the gold electrode on the suede with 0.1 μm α-alumina until the surface is mirror-like, put the gold electrode into secondary water and ultrasonically oscillate for 5 minutes to clean the surface;

[0056] Take 10mL 1.0×10 -3 mol / L o-phenylenediamine solution, 4.0mL 1.0×10 -3 mol / L trichlorfon solution, made into polyfilm bottom solution;

[0057] Put the cleaned gold electrode into the poly-film bottom solution, electropolymerize by cyclic voltammetry, the potential range is 0.8-0V, the scan rate (can rates) is 0.05V / s, and the number of sweep segments (sweep segments) is 15-30. to obtain electropolymers;

[0058] The electropolymer was rinsed with secondary water and allowed to stand for 5 minutes, then eluted with 0.30mol / L NaOH-absolu...

Embodiment 3

[0063] This embodiment provides a preparation method of trichlorfon electrochemiluminescent molecularly imprinted sensor, comprising the following steps:

[0064] (1) Preparation of molecularly imprinted sensors:

[0065] Grind and polish the gold electrode on the suede with 0.05 μm α-alumina until the surface is mirror-like, put the gold electrode into secondary water and ultrasonically oscillate for 4 minutes to clean the surface;

[0066] Take 5mL 1.0×10 -3 mol / L o-phenylenediamine solution, 2mL 1.0×10 -3 mol / L trichlorfon solution, made into polyfilm bottom solution;

[0067] Put the cleaned gold electrode into the poly-film bottom solution, electropolymerize by cyclic voltammetry, the potential range is 0.8-0V, the scan rate (can rates) is 0.05V / s, and the number of sweep segments (sweep segments) is 15-30. to obtain electropolymers;

[0068] The electropolymer was rinsed with secondary water and allowed to stand for 2 minutes, then eluted with 0.2mol / L NaOH-absolute ...

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Abstract

The invention is suitable for the technical field of detection, and provides a dipterex electrogenerated chemiluminescence molecular imprinting sensor as well as a preparation method and application thereof. The preparation method of the dipterex electrogenerated chemiluminescence molecularly imprinted sensor comprises the following steps: preparing the molecularly imprinted sensor: preparing a polymer film base solution from an o-phenylenediamine solution and a dipterex solution; putting the cleaned gold electrode into a polymer membrane base solution, and carrying out electropolymerization through cyclic voltammetry to obtain an electropolymer; eluting the electropolymer by using water, eluting for 3-10 minutes by using a mixed solution of NaOH and absolute ethyl alcohol as an eluent, and eluting the electrode by using water to obtain the molecularly imprinted sensor; preparing a chemiluminescent base solution: uniformly mixing a luminol solution, a PBS (Phosphate Buffer Solution) and a 3% hydrogen peroxide solution to prepare the chemiluminescent base solution. According to the present invention, the dipterex can enhance the luminescence effect on the luminol-H2O2 luminescence system, and the molecular imprinting technology and the electrogenerated chemiluminescence are combined so as to obtain the high-sensitivity and high-selectivity dipterex detection sensor;.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a trichlorfon electrochemiluminescence molecularly imprinted sensor, a preparation method and an application. Background technique [0002] O, O-dimethyl-(2,2,2-trichloro-1-hydroxyethyl) phosphonate, also known as trichlorfon, is the second most commonly used organophosphorus pesticide phosphate, which has stomach toxicity, Broad-spectrum insecticides with contact and penetration are often widely used in pest control of farmland economic crops. Due to the extensive use of trichlorfon, the land and water bodies are polluted by trichlorfon, and not only that, trichlorfon also has a certain amount of damage to the human body. Trichlorfon can inhibit cholinesterase and cause neurophysiological disorders. Short-term exposure to a large amount of trichlorfon can cause headache, vomiting, diarrhea, and fasciculations. In severe cases, pulmonary edema, cerebral edema, para...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N27/30G01N27/48
CPCG01N21/76G01N27/30G01N27/48
Inventor 黎舒怀庞朝海马雄辉
Owner ANALYSIS & TESTING CENT CHINESE ACADEMY OF TROPICAL AGRI SCI
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