Sodium-ibandronate-loaded PLGA microspheres and method for preparing complex-tissue engineering bone employing microspheres

Abstract

The invention relates to the technical field of pharmacochemistry and discloses sodium-ibandronate-loaded PLGA microspheres and a method for preparing complex-tissue engineering bone employing microspheres. The preparation method applying a re-emulsification solvent evaporation method comprises the following steps: dissolving sodium-ibandronate-loaded PLGA into a dichloromethane solution, carrying out mixing through vortex stirring to form a uniform solution, transferring the mixed solution into a beaker containing a PVA aqueous solution, carrying out stirring so as to evaporate the solvent and stabilize a microsphere form, and washing microsphere forms thrice with distilled water, so as to separate and obtain the sodium-ibandronate-loaded PLGA microspheres. According to the microspheres and the preparation method, the sodium ibandronate/PLGA/CPC-loaded complex-tissue engineering bone has good biocompatibility and osteogenesis activity, the adhesion, proliferation and osteogenesis differentiation of mesenchymal stem cells of bone marrow can be promoted, the formation of local new bone of necrotic femoral head is facilitated, the repairing and healing of the necrotic femoral head are accelerated, and thus, the complex-tissue engineering bone can be applied to treatment on ischemic necrosis of the femoral head.

Description

technical field [0001] The invention relates to the technical field of medicinal chemistry, in particular to a PLGA microsphere loaded with sodium ibandronate and a preparation method for a composite tissue engineered bone using the microsphere. Background technique [0002] Necrosis of the femoral head, also known as avascular necrosis of the femoral head (ANFH), is one of the common diseases in orthopedics. It is mainly caused by hormones, rheumatic diseases, blood diseases, etc. It first affects the blood supply of the femoral head, and then causes bone destruction of the femoral head, causing necrosis or even collapse of the femoral head, often causing hip muscle spasm and limited hip joint function , seriously affecting the quality of life of patients. With the development of bone tissue engineering technology, the treatment of osteonecrosis of the femoral head has progressed from the initial single drug or simple operation to comprehensive treatment combined with drug...

AI Technical Summary

Problems solved by technology

[0003] Most of the existing ibandronate drugs are used by injection, and there is a lack of a method that can provide immediate support after minimally invasive cardiac decompression of the femoral head, and at the same time can use ibandronate slow release to promote neonatal Bone formation, improving stress, preventing the collapse of the femoral head, engineering bone for repairing necrotic femoral head