Pharmaceutical composition for resisting swine stress diseases
A composition and anti-stress technology, applied in the field of livestock and poultry drugs, can solve the problems of slow speed, slow absorption, and low oral bioavailability
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Embodiment 1
[0014] Divide the medicinal materials to be decocted into 4 groups, and the combined amount of spider incense and hops in each group is a fixed amount, which contains 60g spider incense and 13g hops; except that the control group has no black pepper added, each group adds black pepper respectively Or black pepper granules 0.05g, 0.1g and 0.5g (hereinafter referred to as the present invention 1, 2, 3), add water to the medicine jar, decoct 3 times after soaking for 1 hour, each 30 minutes. The decoctions were combined and concentrated 10 times for later use.
Embodiment 2
[0016] The Caco-2 cell model is a well-recognized human small intestinal epithelial cell model in vitro. The permeability of drugs in Caco-2 cells has a good correlation with the absorption of drugs in the upper layer of human small intestinal mucosa. Absorption and transport in human intestinal cells, etc. In order to study the effect of different doses of pepper on improving the absorption and bioavailability of the present invention, Caco-2 cells were treated with 2×10 5 / mL cells were inoculated into a transwell-6-well plate and cultured for 21 days. During the cultivation period, the resistance value was detected with a transmembrane resistance meter, when the resistance value of the experimental hole was about 700Ω.cm 2 2.6 mL of the culture system of the present invention was added to the upper chamber, and 2 mL of HBSS solution was added to the lower chamber. -1 ). Samples were taken from the lower layer at 10, 30, and 60 minutes, respectively.
[0017] After the s...
Embodiment 3
[0021] Embodiment 3 The present invention is to the influence of mouse pentobarbital sodium hypnosis
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