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Preparation method and application of Mongolian medicine tribulus terrestris ethanol extract

An ethanol extraction and Tribulus terrestris technology, which is applied in the field of medicine to achieve the effect of reducing the apoptosis rate of liver cells and reducing the index

Inactive Publication Date: 2021-07-20
INNER MONGOLIA UNIV FOR THE NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tribulus terrestris contains saponins, flavonoids, polysaccharides, alkaloids and rich trace elements. At present, there is no relevant report on the preparation of hypoglycemic drugs from the ethanol extract of Tribulus terrestris

Method used

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  • Preparation method and application of Mongolian medicine tribulus terrestris ethanol extract
  • Preparation method and application of Mongolian medicine tribulus terrestris ethanol extract
  • Preparation method and application of Mongolian medicine tribulus terrestris ethanol extract

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Experimental program
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Effect test

Embodiment 1

[0033] Effects of ethanol extract of Tribulus terrestris on metabolism in rats with hyperglycemia

[0034] 1. Materials and methods

[0035] 1.1 Experimental materials, reagents and instruments

[0036] Experimental animals: 100 SPF grade male Wistar rats, weighing (200±20) g, purchased from Liaoning Changsheng Biology Co., Ltd., license number: SCXK (Liao) 2015-0001. Routine feeding, free drinking water, feeding environment light 12h / d, temperature (22±1)°C, relative humidity (45±5)%.

[0037] Experimental drugs: Tribulus, provided by the preparation room of the Affiliated Hospital of Inner Mongolia University for Nationalities, water (water, Watsons); mass spectrometer acetonitrile (Fisher); formic acid (Sigma-Aldrich). D-galactose, CAT, SOD, GSH-Px, MDA kit (Roche Diagnostics Co., Ltd., Germany).

[0038] Main experimental instruments: Vortex, M16710-33, Thermo Scientific; Ultrasonic Washing Device, KQ5200DE, Kunshan Ultrasonic Instrument Co., Ltd.; high speed benchtop r...

Embodiment 2

[0078] Identification of Antioxidative Effects of Ethanol Extract from Tribulus terrestris

[0079]The SOD, MDA, CAT, GSH-Px of different groups of mice were detected, and the results were as follows: Figure 12 shown. SOD, MDA, CAT, and GSH-Px on the oxidative stress pathway change, and drugs have a callback effect on this change. The experimental results are as follows: the blood CAT, serum SOD and GSH-Px indexes of the model rats in the model group were all significantly decreased, and the serum MDA index was significantly increased. However, the indexes of the rats fed with the extract of Tribulus terrestris tended to return to the normal value. There is statistical significance (P<0.01).

Embodiment 3

[0081] Ethanol Extract of Tribulus Terrestris Inhibits Hepatocyte Apoptosis

[0082] Effects of water extract of Tribulus terrestris on apoptosis of model rat liver cells as follows: Figure 13 shown. Compared with the normal control group, the number of apoptotic hepatocytes in the model group increased significantly, with statistical significance (P<0.01). Compared with the model group, the number of hepatic cell apoptosis in the large, medium and small dosage groups of Tribulus terrestris was significantly reduced, which was statistically significant (P<0.01). It shows that the water extract of Tribulus terrestris can inhibit the apoptosis of model liver cells.

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Abstract

The invention discloses a preparation method and application of a Mongolian medicine tribulus terrestris ethanol extract, and belongs to the field of medicines. According to the present invention, the high fat feed induced rat hyperglycemia model is adopted, and the intragastric administration manner verifies that the tribulus terrestris ethanol extract can reduce the hyperglycemia induced by the high fat feed; experimental results show that for hyperglycemia induced by high-fat feed, the tribulus terrestris ethanol extract can improve metabolic component indexes to tend to normal values, improve antioxidant enzyme SOD, CAT and GSH-Px indexes, reduce serum MDA indexes and reduce the liver cell apoptosis rate. The tribulus terrestris ethanol extract has the effect of reducing blood sugar by resisting oxidation and reducing apoptosis expression of hepatocytes. In the invention, a group of 18 biomarkers related to the tribulus terrestris ethanol extract is screened by a metabonomics method, wherein the biomarkers can play a role in separation, research and identification of effective components in the subsequent tribulus terrestris ethanol extract.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method and application of Mongolian medicine Tribulus terrestris ethanol extract. Background technique [0002] Diabetes is a group of metabolic diseases characterized by hyperglycemia. Hyperglycemia is caused by defective insulin secretion or impaired biological action, or both. Long-term high blood sugar leads to chronic damage and dysfunction of various tissues, especially the eyes, kidneys, heart, blood vessels, and nerves. The clinical manifestations are polydipsia, polyuria, polyphagia and diarrhea, fatigue, obesity, etc. Lowering or controlling blood sugar is an important measure to control the development of diabetes. At present, the most direct clinical way is to lower blood sugar through insulin injection, but it is expensive and brings some pain to patients. Therefore, it is necessary to find oral hypoglycemic drugs with low cost and good effect for the prevent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P3/10A61P39/06A61P7/00A61K131/00
CPCA61K36/185A61P3/10A61P39/06A61P7/00A61K2236/333A61K2236/39A61K2236/53A61K2236/51
Inventor 肖田梅
Owner INNER MONGOLIA UNIV FOR THE NATITIES
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