Unlock instant, AI-driven research and patent intelligence for your innovation.

Chemiluminescent substrates for peroxidase with extended shelf-life

A chemiluminescence, peroxide technology, used in chemiluminescence/bioluminescence, biochemical equipment and methods, and analysis by causing chemical reactions to occur in materials, which can solve problems such as not providing experimental evidence

Pending Publication Date: 2021-07-23
CYANAGEN
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Despite the claim that "by stabilizing and / or enhancing hydrogen peroxide in the buffer system, compositions for this type of analysis can have a higher shelf life", no experimental evidence is provided

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chemiluminescent substrates for peroxidase with extended shelf-life
  • Chemiluminescent substrates for peroxidase with extended shelf-life
  • Chemiluminescent substrates for peroxidase with extended shelf-life

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] The kinetics of embodiment 1-sodium perborate oxidation SPTZ and SPOX

[0105] The following solutions were prepared:

[0106] SPTZ solution

[0107] [SPTZ] = 0.05mM

[0108] [Sodium perborate]=4mM

[0109] In 300mM Tris buffer, pH 8.73

[0110]SPOX solution

[0111] [SPOX]=0.05mM

[0112] [Sodium perborate]=4mM

[0113] In 300mM Tris buffer, pH 8.73

[0114] Add a 2 mL aliquot of either solution to a 1 cm quartz cuvette. Insert the cuvette into a thermostatic cuvette holder maintained at 35 °C. Oxidation kinetics were monitored spectrophotometrically at 254 nm (SPTZ) and 312 nm (SPOX) for 64 hours. see results image 3 (SPTZ solution) and Figure 4 (SPOX solution).

Embodiment 2

[0115] Example 2 - Reference Chemiluminescent Substrate

[0116] Two chemiluminescent reference substrates were prepared as follows:

[0117] L / SPTZ1 substrate

[0118] [Luminol]=5.0mM

[0119] [Sodium perborate]=4mM

[0120] [SPTZ]=1.5mM

[0121] In 150 mM Tris buffer, pH 9.0.

[0122] The initial signal is set to 100RLU (relative light unit)

[0123] L / SPTZ2 substrate

[0124] [Luminol]=5.0mM

[0125] [Sodium perborate]=4.0mM

[0126] [SPTZ]=3.0mM

[0127] [MORP]=3.0mM

[0128] In 125 mM Tris buffer, pH 9.0.

[0129] The initial signal of L / SPTZ2 was 10 times higher than that of L / SPTZ1.

Embodiment 3

[0130] Example 3 - Dependence of Chemiluminescence Signal on SPOX Concentration in Luminol / Perborate / SPOX Substrate

[0131] Prepare a series of chemiluminescent substrates with the following composition:

[0132] [Luminol]=5.0mM

[0133] [Sodium perborate]=4mM

[0134] [SPOX] = 0-3.0mM [Enhancer]

[0135] In 125mM Tris buffer, pH 9.0

[0136] 200 μl of these substrates were placed in black 96-well microplates. 30 μl of HRP solution (1 / 3700 ​​of the 20 mg / L solution) was added to each well, and the plate was placed in a Wallac Victor-3 plate reader.

[0137] With reference substrate L / SPTZ1 (embodiment 2) normalized initial chemiluminescent signal that is set as 100RLU is plotted relative to SPOX concentration, as Figure 5 shown.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A kit for performing an assay for determining an analyte in a sample with an extended shelf-life, wherein the kit comprises a chemiluminescent cyclic dihydrazide, an enhancer, a co-enhancer, and a peroxide oxidizer. The kit is useful in blot assays and immunoassays for the detection of proteins and nucleic acid molecules.

Description

technical field [0001] The present invention relates to a chemiluminescent substrate for the determination of peroxidase in an assay for the determination of an analyte in a sample, wherein the chemiluminescent substrate has an extended shelf life. Background technique [0002] The chemiluminescent oxidation of luminol catalyzed by horseradish peroxidase (HRP) is widely used in the analysis and testing of antigens, antibodies and nucleic acids, especially blot tests, such as dot blot and Western blot (protein), Southern blot and Northern blot ( nucleic acids) and enzyme-linked immunoassays (EIA, for proteins or nucleic acids). [0003] It is known that the HRP-catalyzed chemiluminescent oxidation of luminol can be made faster and more efficient by adding electron mediators or enhancers, as for example Kricka LJ (1991), Clinical Chemistry, 37:1472-1481 or KrickaLJ, Voyta JC and Bronstein I in "Chemiluminescent Methods for Detecting and Quantitating Enzyme Activity" (2000), M...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/07G01N21/76
CPCC09K11/07G01N21/76C09K2211/1044C09K2211/1059G01N33/582C12Q1/28G01N21/78G01N33/573G01N2333/908G01N2458/00
Inventor L·德拉·西亚纳L·比亚吉尼T·P·扬森R·佩尔西亚坎特M·瓦焦鲁M·E·韦特拉伊诺
Owner CYANAGEN
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com