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Porcine alveolar macrophage healthy cell line and construction method and application

A technology of alveolar macrophages and healthy cells, which is applied in the biological field to achieve the effects of repairing tissues, promoting wound healing and fibrosis, and promoting tumor growth and infiltration

Pending Publication Date: 2021-07-23
BLUESKY BIOTECH (TAIZHOU) PARTNERSHIP (LLP)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The healthy cell line of porcine macrophages is the best choice, but there is no healthy cell line of porcine macrophages obtained by natural domestication in the laboratory without any lentivirus transformation or any plasmid transfection

Method used

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  • Porcine alveolar macrophage healthy cell line and construction method and application
  • Porcine alveolar macrophage healthy cell line and construction method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0047] Embodiment: This example provides the method and process of porcine alveolar macrophage healthy cell line not using any tumorigenic virus infection or any recombinant plasmid transfection with tumorigenic gene:

[0048] 1. Establishment of healthy porcine alveolar macrophage cell line

[0049] The healthy piglets were culled, and the healthy piglets were 8-week-old SPF piglets without 14 kinds of pathogens, purchased from Harbin Guosheng Biotechnology Co., Ltd. These 14 pathogens include: swine fever virus, porcine circovirus type 2, porcine reproductive and respiratory syndrome virus, porcine epidemic diarrhea virus, porcine transmissible gastroenteritis virus, porcine parvovirus, bovine viral diarrhea virus, Foot-and-mouth disease virus, mycoplasma hyopneumoniae, porcine rotavirus, porcine pseudorabies virus antigen antibody double negative; African swine fever virus, Japanese encephalitis virus, swine influenza virus antigen negative.

[0050] PAM cells were obtaine...

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PUM

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Abstract

The invention discloses a healthy porcine alveolar macrophage cell line and a construction method and application. The collection number of the healthy porcine alveolar macrophage cell line is CGMCC No: 21498. According to the healthy porcine alveolar macrophage cell line, the 100% healthy porcine alveolar macrophage cell line is screened by adopting a special cell culture solution in combination with a natural domestication selection method, and the healthy porcine alveolar macrophage cell line can be applied to cell culture of viruses, research, development and production of live vaccines and inactivated vaccines and preparation of bioactive substances secreted by M1 and M2 macrophages after induced polarization, and the bioactive substances are used for cancer treatment, inflammation diminishing and the like. According to the invention, the healthy porcine alveolar macrophage cell line is cultured for the first time, any carcinogenic substances such as lentivirus transformation or plasmid transfection are not adopted in the construction method of the invention, and the constructed healthy porcine alveolar macrophage cell line keeps the characteristics of primary porcine alveolar macrophages.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a healthy porcine alveolar macrophage cell line, a construction method and application. Background technique [0002] Macrophages are one of the important immune cells in the body. Porcine alveolar macrophages (PAM) are an important part of the body's resistance to pathogenic infection. They not only have the functions of phagocytosis and antigen presentation, but also secrete a large amount of biologically active substances, which can improve the immune inflammatory response and inhibit inflammation. happened. Porcine alveolar macrophages are also the target of infection and attack by many viruses, so these viruses and corresponding vaccine viruses can replicate and multiply in large numbers in porcine alveolar macrophages, such as African swine fever virus, porcine reproductive and respiratory syndrome virus, porcine Epidemic diarrhea virus, porcine circovirus, swine flu virus, e...

Claims

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Application Information

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IPC IPC(8): C12N5/0786C12N7/00A61K35/15A61P35/00A61P37/04A61P29/00C12R1/91C12R1/93
CPCC12N5/0645C12N7/00A61K35/15A61P35/00A61P37/04A61P29/00C12N2509/00C12N2501/052C12N2501/24C12N2501/2304C12N2501/2313C12N2770/20051Y02A50/30
Inventor 崔现兰崔楼明崔楼耀楼冰
Owner BLUESKY BIOTECH (TAIZHOU) PARTNERSHIP (LLP)
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