37 results about "Double negative" patented technology

The invention discloses a double negative curvature anti-resonance hollow-core optical fiber. The optical fiber comprises a cladding region and a fiber core region, the cladding region sequentially comprises an outer cladding, a first anti-resonance layer and a second anti-resonance layer from outside to inside, wherein the first anti-resonance layer comprises multiple rotatably and symmetricallydistributed first capillaries, and the first anti-resonance layer is tangent to the outer cladding; the second anti-resonance layer comprises multiple rotatably and symmetrically distributed second capillaries, the second capillaries and the first capillaries are tangent to each other and in staggered arrangement, and the radiuses of the second capillaries are smaller than the radiuses of the first capillaries; and the second anti-resonance layer is closer to a center line of the optical fiber compared with the first anti-resonance layer, and an irregular region surrounded by boundaries of thefirst anti-resonance layer and the second anti-resonance layer is namely the fiber core region. The invention aims at solving the technical problems that small curvature radius boundary advantage ofan anti-resonance negative curvature hollow-core optical fiber is not fully played, a high order mode loss ratio is low, structural uniformity can not be guaranteed and anti-resonance structure preparation difficulty is high in the prior art.

The present invention disclooses the preparing process of nano level superfine tourmalline microcrystal with negative ion generating function. The process may obtain superfine tourmaline microcrystalwith stable size and doubled negative ion generating function. The present invention may be used in raising the negative ion density in air to create optimized living environment.

The invention relates to a compound solid oxide fuel cell, which consists of a compound positive electrode layer, an oxygen ion-proton double-conductor compound electrolyte layer, a compound porous electrolyte interlayer and a double-negative-electrode layer, wherein the compound electrolyte layer can simultaneously carry out proton electrical conduction and oxygen ion electrical conduction, and electrolyte ion electrical conduction can achieve performance requirements at intermediate, low and high temperatures; the compound porous electrolyte interlayer can effectively obstruct reaction between the negative electrode and the electrolyte so as to lower interfere resistance; and the double-negative-electrode layer consists of an electron electrical conduction enhancement layer and an ion electrical conduction enhancement layer, and polarization resistance is greatly lowered. Therefore, the compound solid oxide fuel can meet performance requirements at any SOFC (Solid Oxide Fuel Cell) operation temperature (350-800DEG C), and has a wide application value.

The invention discloses a noise deadener and an air conditioning system. The noise deadener comprises a shell, an input pipe, an output pipe and multiple resonance units, wherein the input pipe and the output pipe are arranged outside the shell; and the multiple resonance units are arranged inside the shell and are tubular. The input pipe and the output pipe are connected to opposite side walls of the shell correspondingly. The resonance units are distributed in a periodic manner and extend in the transverse direction of the input pipe. Openings are formed in the pipe walls of the resonance units. According to the noise deadener and the air conditioning system, the multiple tubular resonance units are arranged in the shell, and wideband response double negative parameters can be achieved. By means of the noise deadener, the low-frequency noise deadening amount can be increased; and by adjusting the conduction rate of the resonance units, a phononic band gap can be made to move toward the low frequency, and thus a good effect can be obtained in the aspects such as reduction of low-frequency noise such as noise of a compressor.

The invention discloses a construction method of a chimeric antigen receptor double-negative T cell. The construction method comprises the following steps: according to characteristics of CD3<+> / CD4<-> / CD8<->double-negative T cell, selecting the purified CD3<+> / CD4<-> / CD8<->double-negative T cell in vitro, and utilizing the synergistic principle of multiple cell factor signal channels and a TCR signal channel to screen out an in-vitro culture scheme suitable for activation and virus transfection of the double-negative T cell. The in-vitro culture scheme can quickly and controllably activate the double-negative T cell, expresses the specific chimeric antigen receptor on the double-negative T cell through a lentiviral transfection technology at high efficiency, preparing a CAR-DN-T cell, enables the CAR-DN-T cell to specifically identify the tumor cell corresponding to the chimeric antigen receptor, promotes the CAR-DN-T cell to be further activated and proliferated, and quickly kills and wounds the tumor cell. According to the construction method disclosed by the invention, the prepared CAR-DN-T cell is from homologous variants of other healthy donators, so that the preparation of the cell gets rid of limitation of the current illness situation of a patient, the cell can be prepared on a large scale in advance, and the patient can be subjected to low-dosage re-transfusion treatment for multiple times according to the clinical diagnosis result at any time.

A method of expanding double negative T cells in culture is described. The method comprises (a) providing a starting sample comprising DN T cells or precursors thereof; (b) substantially depleting CD8+ and CD4+ T cells from the starting sample; (c) culturing the sample from step (b) with an immobilized T cell mitogen in a culture medium comprising an agent that can stimulate DN T cell growth; (d) washing the cells obtained in step (c) and resuspending in a culture medium comprising the agent without the T cell mitogen; and (e) washing the cells obtained in step (d) and resuspending in a culture medium comprising the agent and a soluble T cell mitogen. The DN T cells obtained by the method are useful in a variety of applications including the treatment of cancer, infectious diseases, graft versus host disease and autoimmune disease.

A method and material for increasing the sensitivity of near-field probes used in detecting a wide variety of materials and objects such as biological anomalies in tissues, cracks on metallic surfaces, composition of material such as permittivity and permeability . . . etc., is disclosed. The present invention includes having a metamaterial in front of near-field probes that result in increased sensitivity. The metamaterial to be placed in the presence of the near-field probe has electrical characteristics that can be described as single negative or double negative media. Once the single negative or double negative medium is placed in the close proximity or between the material to be investigated and the near-field probe, the sensitivity of the near-field probe to variation in the detected object or material will be enhanced. This invention is useful when the near-field probe is insensitive enough not to detect small variation in the composition or geometry of the target.

A one-step method for producing antigen loaded antigen-presenting cells from monocytes ex vivo has been found which comprises contacting the monocytes with a composition comprising an activator such as TNF alpha preferably in combination with at least one growth factor such as GM-CSF and at least one soluble or particulate antigen. According to the methods of the present invention, antigen-loaded dendritic cell vaccines can be generated within as little as three (3) days. In another method of the present invention, antigen loaded antigen-presenting cells are produced from monocytes ex vivo by contacting the monocytes with TNF alpha and granulocyte-macrophage colony stimulating factor at one time point to form antigen-presenting cells and then contacting antigen-presenting cells with soluble or particulate antigenic material antigen-presenting cells, wherein the antigen loaded antigen-presenting cells are produced in less than four days. The present invention also includes a vaccine which comprises monocyte-derived antigen loaded antigen-presenting cells, wherein the antigenpresenting cells are composed of two or more subsets selected from the group consisting of Langerhans cells with surface markers (CD 1 a+CD207+); interstitial dendritic cells with surface markers (CD 1a+CD207−); double negative dendritic cells with surface markers 20 (CD 1 a−CD 14−); and dendritic cells with surface markers (CD 14+CD 1 a−CD209+).

The invention relates to a K-waveband electromagnetic double-negative metamaterial, which comprises a dielectric substrate, a novel resonator and a rectangular metal lead wire, wherein the novel resonator and the rectangular metal lead wire cover the dielectric substrate; the novel resonator comprises a structure whose inside is a closed regular triangle combined by three metal strips and outside is a regular hexagon with three openings; the closed triangle is coincided with three vertexes of the hexagon; and each of the three vertexes, which are not coincided with the triangle, of the hexagon is provided with one opening, overall representing a back-to-back combination of three small resonant cavities. Based on the traditional structure combined with the resonant cavities and the rectangular metal lead wire, the novel resonator is proposed; according to the resonator, through a manner of parallel connection of the relatively small resonant cavities, the resonant frequency is improved, and a novel double-negative material is formed by combining with the rectangular metal lead wire; the material not only realizes the double-negative characteristic at 18.8Ghz (K-waveband), but also guarantees the radiating area, so that the material can have the better double-negative characteristic when being applied to an electromagnetic device.

A pulse excitation bistable electromagnetic actuator is used in fields such as relays, contactors, circuit breakers, solenoid valves, and electromagnets. Using pulse current excitation to actuate, magnets or retaining rings, spring pins, springs, etc. maintain a steady state, eliminating the need for prior art - positive and negative bi-directional pulse generation circuits and winding changeover switches in magnetic holding bistable; or in mechanical bistable Complicated bistable mechanism and return force spring can switch between two stable states only by inputting one-way pulse successively with one switch. It has the advantages of energy saving, anti-vibration, simple structure, easy miniaturization, not easy to shake, and long working stroke. features. It consists of a machine base, an electromagnet containing moving parts and a device for maintaining the steady state of the moving parts. It can be freed from the constraints of the steady-state maintaining device by the pulsed force of the electromagnet, swing from one side to the other side by relying on inertia to cross the point of maximum magnetic attraction, and be in a stable state again under the action of the steady-state maintaining device.

The invention relates to disposable sanitary production line equipment, in particular to an adhesive tape cutter device with inserts interchangeable. Two groups of negative pressure holes are formed in a bottom roller and are symmetrical relative to the bottom roller, a locating face is formed on the part, between the two groups of negative pressure holes, of the bottom roller, a group of protruding locating pillars are arranged on the locating face, the negative pressure holes are communicated with a negative pressure plate, a negative pressure box is provided with two negative pressure openings, the negative pressure openings are connected with the negative pressure plate through negative pressure pipes, and a cutter system and a cam bonding system correspond to one group of negative pressure holes respectively. According to the structure of the adhesive tape cutter device, the bottom roller is provided with double negative-pressure suction structures, the locating pillars are arranged between the two groups of negative pressure holes, in the material conveying process, even if the negative pressure holes are not covered, materials cannot be subjected to sliding or displacement on the bottom roller under the action of the locating pillars, the areas of the negative pressure holes are decreased, and power of a vortex air pump can be reduced.

The invention discloses a triple-band antenna with gaps which are loaded nested double R-shaped left-handed structures, and relates to a triple-band antenna. The triple-band antenna is provided with a dielectric substrate. A rectangular patch is etched on the upper surface of the dielectric substrate, two loaded nested R shaped left-handed structures which are used as the gaps are loaded on a monopole antenna, feed effects can be realized by planar waveguides, and arcs are loaded on the left edge and the right edge of a floor. The triple-band antenna has the advantages that the shortcomings of narrow double-negative frequency bands, high loss and large sizes of existing microwave-band left-handed materials can be overcome by asymmetric left-handed material unit structures, and the asymmetric left-handed material unit structures can be applied to micro-strip antennas, are flexible and controllable in electric resonance and magnetic resonance frequency range, wide in left-handed characteristic bandwidth and low in loss and have special electromagnetic characteristics and simple left-handed characteristic regulation parameters as compared with symmetric left-handed materials owing to the asymmetry of the left-handed material unit structures in incident directions of electromagnetic waves.

An isotropic double-negative artificial material belongs to the technical field of functional materials. The invention is formed by densely stacking the double-negative artificial material units of the three-dimensional structure periodically; the double-negative artificial material unit of the three-dimensional structure comprises a medium material cube and double-negative structural units located on three adjacent faces of the medium material cube; the double-negative artificial material unit The structural unit includes a dielectric substrate, a metal open-circuit ring resonator and a thin metal wire; the metal open-circuit ring resonator is located on the front of the dielectric substrate, and consists of two open-circuit rings with different sizes and opposite openings, in which the small open-circuit ring is nested in the large open-circuit ring; The thin metal wires are located on the back of the dielectric substrate, parallel to the opening center line of the two split rings and the distance is equal to the thickness of the dielectric substrate; in the double-negative artificial material unit of the three-dimensional structure, the metal thin wires of the three double-negative structural units are perpendicular to each other. The double-negative artificial material provided by the invention has the characteristics of three-dimensional structure and isotropy, and can be applied to high-power microwave and millimeter wave sources for exciting microwave and millimeter waves.

The invention provides a cell phone antenna and a cell phone, wherein the cell phone antenna comprises a dielectric substrate, a rectangular conductive patch which is arranged on one side of the dielectric substrate and a metal floor which is arranged on the other side of the dielectric substrate, wherein the metal floor is provided with double negative material, and a double negative material resonant cavity is formed by the double negative material, the rectangular conductive patch and the dielectric substrate, and the double negative material resonant cavity is along the width which is less than the distance between two radiating sides of the rectangular conductive patch and is parallel to the dielectric substrate, the interface of the double negative material resonant cavity and a double positive material resonant cavity which is fixedly provided by the dielectric substrate is parallel to the two radiating sides of the rectangular conductive patch and is crossed with two non-radiating sides of the rectangular conductive patch. The cell phone antenna can greatly reduce the electric size of an antenna under the premise of guaranteeing necessary radiation efficiency and transmission gain.

The inventors demonstrate that treatment of young, suckling mice with a glycolipid derived from Helicobacter pylori activates NKT cells in a CD1d-restricted fashion, and is protective against AHR in a model of allergen-induced asthma. The inventors further found that this protective effect can be transferred by NKT cells exposed to the glycolipid, and is associated with the expansion of a suppressive double-negative NKT cells and Foxp3+ TReg cells. The inventors also demonstrate herein that pretreatment of adult mice with a glycolipid derived from Helicobacter pylori partially suppresses airway hyperreactivity and inhibits BAL inflammation in an ozone-exposure model. Accordingly, provided herein are compositions and methods for the treatment and prevention of inflammatory diseases, such as asthma or autoimmune diseases, in a subject in need thereof.

There is provided herein methods of treating leukemia or lymphoma in a subject in need thereof, with double negative T cells (DNTs) in combination with Interferon-γ.

A method of expanding double negative T cells in culture is described. The method comprises (a) providing a starting sample comprising DN T cells or precursors thereof; (b) substantially depleting CD8+ and CD4+ T cells from the starting sample; (c) culturing the sample from step (b) with an immobilized T cell mitogen in a culture medium comprising an agent that can stimulate DN T cell growth; (d) washing the cells obtained in step (c) and resuspending in a culture medium comprising the agent without the T cell mitogen; and (e) washing the cells obtained in step (d) and resuspending in a culture medium comprising the agent and a soluble T cell mitogen. The DN T cells obtained by the method are useful in a variety of applications including the treatment of cancer, infectious diseases, graft versus host disease and autoimmune disease.

The invention discloses a double-negative-oxygen-ion and double-ring-leakage life sanitation product which comprises a thin-pore skin-friendly soft cotton layer, a negative oxygen ion plant fiber layer, a magnetic powder attached magnetic nonwoven fabric or magnetic fabric layer, far-infrared nonwoven fabric or an far-infrared fabric layer, a lithospermum and / or lavender spice attached nonwoven aromatic layer or fabric aromatic layer, a tourmaline negative oxygen ion particles attached antiseptic layer, a moisture locking balls attached nonwoven moisture locking layer and a water-absorbing material attached nonwoven water absorbing layer and permeable bottom membrane layer which are arrayed sequentially from inside to outside. A hourglass type suspension core structure warping annularly is adopted, multiple elastic ribs are arranged in the suspension core structure and are connected with an infrared signal transmitter through an electrolytic patch and a lead, the electrolytic patch sends an alarm signal to a signal receiver after connected through the wetted life sanitation product, the receiver transmits the alarm signal to a Bluetooth module after receiving the alarm signal, and the Bluetooth module transmits the alarm signal to a matched mobile phone through a wireless router.

The present invention provides a human cervical adenocarcinoma cell line, which is characterized by naming as a primary cervical adenocarcinoma drug-resistant strain W038, and is deposited in the China Center for Type Culture Collection with a preservation number being CCTCC No: C2017246. The primary cervical adenocarcinoma drug-resistant strain W038 has weak invasion ability and weak migration ability, and can be used as a double-negative control group for invasion and migration. The in-vitro soft agar colony formation test proves that the tumorigenicity proves that the cloning ability is strong, and the in-vitro susceptibility test that the strain is resistant to a variety of drugs, which is consistent with clinical features. The preparation method disclosed by the invention can purify cancer cell lines to over 99%, wherein the screened medium component can selectively kill histocyte, has high selectivity for tumor cell growth, and can increase the proliferation rate of tumor cells and shorten the culture period. The method can be used not only to purify the cervical adenocarcinoma lines, but also to purify and prepare other cancer cell lines.

The invention discloses a double-negative pressure sputum aspiration device, comprising a sputum aspiration pipeline (1), a transitional connection component (2), a sputum storage bag (3), a shell (4) and a negative pressure channel (5), wherein the sputum storage bag (3) is connected with the negative pressure channel (5) through the transitional connection component (2) and the sputum aspiration pipeline (1), the sputum storage bag (3) is arranged inside the shell (4), and a gap (4-1) from the sputum storage bag (3) to the inner wall of the shell (4) is connected with the shell (4). In the device, based on the principle of double negative pressures, the soft and small sputum storage bag (3) is arranged inside the shell (4) to replace a hard and large sputum storage bottle (8), thereby overcoming the defects of traditional sputum aspiration device.

The invention discloses a two-way negative pressure forming needle bed, which comprises: a cabinet assembly and a power supply assembly; the cabinet assembly is formed by stacking a plurality of storey layers, and the storey layer includes: a bottom plate assembly; an upper plate arranged above the bottom plate assembly Components; the middle plate component that is movably arranged between the bottom plate component and the upper plate component; the negative pressure component that is set on the upper plate component and used to form the battery; the negative pressure component is set as a double negative pressure component, and includes: negative pressure The cup module and the negative pressure manifold connected with the negative pressure cup module; the negative pressure cup module, the negative pressure manifold and the preset negative pressure control system form a double negative pressure loop. The invention adopts double negative pressure circuits to form the battery, and forms a uniform, stable and dense SEI film on the negative electrode of the lithium ion battery under the negative pressure environment, so that the performance of the battery is more stable and the quality of the battery is improved.

The invention discloses a hydrogen-nickel battery negative electrode sheet and a method for manufacturing a hydrogen-nickel battery using the same. The negative electrode sheet is a double negative electrode sheet, and the manufacturing method comprises welding a single negative electrode short sheet at the end of a single negative electrode long sheet through a negative electrode connection substrate. Double negative electrode sheet, first weld a small piece of protective film with a width larger than the width of the negative electrode connection substrate on the diaphragm corresponding to the connection seam of the double negative electrode sheet, and then place the diaphragm welded with the protective film between the positive and negative electrode sheets, and then place the The double negative plate, diaphragm, and positive plate are rolled into a cylindrical shape and loaded into the battery case, and finally the nickel-hydrogen battery is assembled by injecting electrolyte, installing a cap and sealing ring. It can effectively control and manufacture pole pieces with different thicknesses and different amounts of negative electrode active materials, improve the utilization rate of negative electrode active materials and the performance of the battery, correspondingly reduce the thickness of double negative electrode pieces and occupy the volume of the shell, and reduce the battery life. The manufacturing cost and assembly difficulty significantly improve the manufacturing efficiency of the battery, and are especially beneficial to the manufacture of high-capacity batteries.

The invention discloses a tetracyclic compound capable of promoting in-vitro proliferation of double-negative T cells. The tetracyclic compound is IMC-00239, and the molecular formula of the tetracyclic compound is shown in the description. The invention relates to the tetracyclic compound which can be used for promoting in-vitro proliferation of the CD3<+> / CD4<-> / CD8<->double-negative T (DN T) cells and is selected from a compound library according to a high-throughput screening method, and provides a pharmaceutically acceptable salt and a prodrug of the tetracyclic compound. The compound is a restrainer of a Wnt signal channel, and can induce degradation of beta-Catenin of the DN T cells, shorten cell cycle and promote karyokinesis when being used in in-vitro experiments. The compound can effectively promote the construction of a CAR-DN-T in-vitro proliferation system, and help implementation of a CAR-DN-T cell factory.

The invention discloses a TCR- / PD-1-double negative T cell and its construction method. The present invention activates isolated peripheral blood mononuclear cells into T cells, knocks out TCR and PD-1 through CRISPR / Cas9 gene editing technology, and sorts out TCR- / PD-1-double negative T cells through magnetic beads. The TCR- / PD-1-double negative T cells prepared by the present invention can be used for adoptive cell immunotherapy of tumors, including DC-CIK, CTL, TIL, CART, etc. The TCR- / PD-1-double-negative T cells of the present invention can be derived from other healthy donors of the same type. Therefore, the application of the cells in adoptive cell therapy not only gets rid of the limitations of the patient's current disease status, but can also be used on a large scale in advance. preparation, reducing treatment costs.

Methods for the treatment of cancer using double negative (DN) T cells are described. The DNTs may be used for the treatment of chemotherapy-resistant cancers such as recurring or relapsing acute myeloid leukemia (AML). The use of allogenic DNTs, such as those derived from healthy donors, that do not exhibit toxicity towards normal host tissues and the complications associated with graft-versus-host-disease, is also described.

The inventors demonstrate that treatment of young, suckling mice with a glycolipid derived from Helicobacter pylori activates NKT cells in a CD1d-restricted fashion, and is protective against AHR in a model of allergen-induced asthma. The inventors further found that this protective effect can be transferred by NKT cells exposed to the glycolipid, and is associated with the expansion of a suppressive double-negative NKT cells and Foxp3+ TReg cells. The inventors also demonstrate herein that pre-treatment of adult mice with a glycolipid derived from Helicobacter pylori partially suppresses airway hyperreactivity and inhibits BAL inflammation in an ozone-exposure model. Accordingly, provided herein are compositions and methods for the treatment and prevention of inflammatory diseases, such as asthma or autoimmune diseases, in a subject in need thereof.