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Application of insecticidal protein in prevention and treatment of aphids

A technology of insecticidal protein and pests, applied in the direction of application, insecticide, biocide, etc., can solve problems such as environmental pollution, food safety, collateral damage, etc.

Active Publication Date: 2021-07-30
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The long-term reliance on chemical pesticides for aphid control has brought many problems, such as collateral damage to non-target organisms (such as natural enemy insects, economic insects and other non-target insects, humans and animals), environmental pollution, food safety, etc.

Method used

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  • Application of insecticidal protein in prevention and treatment of aphids
  • Application of insecticidal protein in prevention and treatment of aphids

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Experimental program
Comparison scheme
Effect test

preparation example Construction

[0025] Table 1 is the preparation table of SDS polyacrylamide gel.

[0026] Table 1 Preparation table of SDS polyacrylamide gel

[0027]

Embodiment

[0029] The insecticidal protein gene Cry78Aa1 (SEQ ID No.1, the amino acid sequence of the protein encoded by it is shown in SEQ ID No.2) gene in IPPBIOTSUC-B4F11 was connected to pET21b, and transformed into Escherichia coli Rosetta (DE3) , and the transformant was named Rosetta(DE3)-78A.

[0030] Extraction of insecticidal protein:

[0031] (1) Pick a single colony of Rosetta(DE3)-78A and put it in 5mL LB liquid medium (adding ampicillin and chloramphenicol), culture at 220rpm at 37°C, and wait for 2h from OD to OD 600 When the temperature is between 0.5 and 0.8, add 500 μL of 1mol / L IPTG;

[0032] (2) Adjust the temperature to 20° C., 150 rpm, and continue culturing for 12 hours to obtain a fermentation broth;

[0033] (3) Centrifuge the fermentation broth at 8000g for 10min at 4°C;

[0034] (4) Discard the supernatant, resuspend the pellet with 10mL 20mmol / L pH=8.0 Tris-HCl buffer, transfer to a 50mL centrifuge tube, and ultrasonically disrupt the cell wall (power 60%, ...

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Abstract

The invention belongs to application of insecticidal protein in prevention and treatment of aphids. The amino acid sequence of the insecticidal protein is shown as SEQ ID No.2.

Description

technical field [0001] The invention relates to the application of insecticidal protein in controlling aphids. Background technique [0002] Aphids have a wide variety of species and a wide range of hosts, many of which are important agricultural pests. It feeds on thorns and feeds on the phloem juice of host plants, and causes serious losses in the yield and quality of host crops by plundering host nutrients, carrying and spreading plant viruses, and excreting honeydew. [0003] Wheat aphids are the most important pests of wheat in my country. Every year, the wheat aphids caused by wheat aphids reduce the yield of wheat by 10% to 40%, which seriously threatens the production of wheat and food security in our country. Sitobion miscanthi belongs to Hemiptera Aphididae and is the dominant species of aphids in various wheat areas in China. It was mistakenly identified as Sitobion avenae in China and is still widely used. Dichotia aphid is an oligophagous piercing-sucking pest...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N37/46A01P7/04
CPCA01N37/46
Inventor 张杰范佳曹蓓蓓耿丽丽王泽宇束长龙
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI