Application of konjac polysaccharide degradation products kgm-1k and kgm-5k in the preparation of probiotic protective agent

A konjac polysaccharide and degradation product technology is applied in the biological field to achieve the effects of enhanced stress resistance, improved survival rate and improved biological efficacy

Active Publication Date: 2022-08-09
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether the degradation products of konjac polysaccharides have protective effects on probiotics has not been reported

Method used

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  • Application of konjac polysaccharide degradation products kgm-1k and kgm-5k in the preparation of probiotic protective agent
  • Application of konjac polysaccharide degradation products kgm-1k and kgm-5k in the preparation of probiotic protective agent
  • Application of konjac polysaccharide degradation products kgm-1k and kgm-5k in the preparation of probiotic protective agent

Examples

Experimental program
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Effect test

Embodiment 1

[0054] This example studies the effect of konjac polysaccharide degradation product KGM-1k on the survival of Bifidobacterium longum SQ011.

[0055] Preparation of simulated gastric juice containing KGM-1k, a degradation product of konjac polysaccharide: first prepare MRS medium without carbon source, adjust the pH of the MRS medium without carbon source to 2 with hydrochloric acid, add 5% (KGM- 1k / MRS medium, m / m) KGM-1k, a degradation product of konjac polysaccharide, was fully dissolved and then autoclaved at 121°C. Then, pepsin was dissolved in an appropriate amount of PBS solution (phosphate buffered solution) and sterilized by filtration; finally, the treated pepsin was added at a concentration of 3 g / L to the above-mentioned MRS medium containing KGM-1k, a degradation product of konjac polysaccharide. .

[0056] Preparation of simulated small intestinal fluid containing KGM-1k, a degradation product of konjac polysaccharide: first, the pH of the MRS medium without carb...

Embodiment 2

[0071] This example studies the effect of konjac polysaccharide degradation product KGM-5k on the survival of Bifidobacterium longum SQ011.

[0072] Preparation of simulated gastric juice containing KGM-5k, a degradation product of konjac polysaccharide: first prepare MRS medium without carbon source, adjust the pH of the medium to 2 with hydrochloric acid, add 5% (KGM-5k / MRS medium after packaging) , m / m) of konjac polysaccharide degradation product KGM-5k, fully dissolved and then autoclaved at 121 °C. Pepsin was then dissolved in an appropriate amount of PBS solution and filter sterilized. Finally, the treated pepsin was added to the above-mentioned MRS medium containing KGM-5k, a degradation product of konjac polysaccharide, at a concentration of 3 g / L.

[0073] Preparation of simulated small intestinal fluid containing KGM-5k, a degradation product of konjac polysaccharide: first, the pH of the MRS medium without carbon source was adjusted to 7 with sodium hydroxide, and...

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Abstract

The invention relates to the application of konjac polysaccharide degradation products KGM-1k and KGM-5k in the preparation of a probiotics protective agent. In the present invention, hydrogen peroxide is first used to destroy the hydrogen bond between the konjac polysaccharide molecules, then cellulase is used to degrade it, and 60% ethanol alcohol precipitation is separated and purified to prepare the konjac polysaccharide degradation product KGM-with uniform molecular weight 1k and KGM‑5k components. The effects of konjac polysaccharide degradation products KGM-1k and KGM-5k on the survival of probiotics were investigated for the first time under simulated gastric juice, small intestinal juice, vacuum freeze-drying and storage conditions at different storage temperatures (4℃ and 25℃). The results showed that KGM-1k and KGM-5k, the degradation products of konjac polysaccharide, could enhance the stress resistance of probiotics, improve the number of viable bacteria and the survival rate in the digestive tract of probiotics under vacuum freeze-drying and storage conditions at different storage temperatures. Furthermore, it can tolerate gastrointestinal juices entering the predetermined part of the intestine to achieve colonization, which is of great significance for improving the biological efficacy of probiotics.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of konjac polysaccharide degradation products KGM-1k and KGM-5k in the preparation of a probiotics protective agent. Background technique [0002] Probiotics refer to active bacteria or yeasts that are beneficial to human health, especially the health of the digestive system. They can inhibit the growth of harmful bacteria, protect the gastric mucosa from damage, improve the body's immunity, prevent diarrhea, maintain stable intestinal function, and promote gastrointestinal motility. Aids digestion and laxative functions. When the intestinal flora is imbalanced, the structure of the flora can be adjusted and repaired by oral probiotics. However, before these probiotics can colonize the large intestine, they first need to be transported through the stomach and small intestine, and gastric juice and pepsin in the stomach and trypsin and choline salts in the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/04C12P19/14C12P19/00C12R1/125C12R1/01C12R1/225C12R1/145
CPCC12N1/20C12N1/04C12P19/14C12P19/00Y02A50/30
Inventor 王仲孚龚桂萍黄琳娟方洁王雪
Owner NORTHWEST UNIV
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