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Method for preparing feed probiotic agent from rice flour wastewater and passion fruit peel by lactic acid bacteria

A technology of passion fruit peel and lactic acid bacteria, applied in the direction of bacteria, etc., can solve problems such as probiotics that have not yet appeared, and achieve the effects of enhancing animal immunity, facilitating absorption, and realizing reuse.

Pending Publication Date: 2021-08-06
南宁市拜欧生物工程有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the current reports, there is no precedent for mixing the rice flour wastewater and passion fruit peel with probiotics for fermentation to produce feed probiotics

Method used

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  • Method for preparing feed probiotic agent from rice flour wastewater and passion fruit peel by lactic acid bacteria
  • Method for preparing feed probiotic agent from rice flour wastewater and passion fruit peel by lactic acid bacteria
  • Method for preparing feed probiotic agent from rice flour wastewater and passion fruit peel by lactic acid bacteria

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] In this example, the inoculation ratio of lactic acid bacteria was investigated, and the fermentation experiment was carried out in the culture medium inserted into rice flour wastewater and passion fruit peel according to different (W / W) inoculation amounts, and the fermentation time was 24-36h. The inoculation ratios were 2%, 4%, 6%, 8%, and 10%, respectively, and the total colony count of the fermentation product was measured. The results are shown in Table 1. Known from Table 1, the optimum seed solution ratio is 6%.

[0025] Table 1 Effects of different strain ratios on the physical and chemical indicators of fermentation products

[0026]

Embodiment 2

[0028] In the present invention, the fermentation time is investigated, and the liquid seed solution with a lactic acid bacteria inoculation ratio of 6% is inserted into the liquid medium of rice flour wastewater and passion fruit peel, and the fermentation temperature is 37°C, as shown in Table 2 below.

[0029] Table 2 Effects of different fermentation times on the physical and chemical indicators of fermentation products

[0030]

Embodiment 3

[0032] The present invention investigates the feeding of probiotics in piglets. Add the lactic acid bacteria into the liquid culture medium of rice flour wastewater and passion fruit peel according to the inoculation amount of 6% (W / W), ferment the temperature at 37° C., and ferment for 36 hours to obtain the probiotics for feeding. Common pig feed is used as a blank control group, and the fermented feed of the present invention is used as a test group for testing. The piglets had free access to food and were fed for 1 month. The feeding results are shown in Table 3.

[0033] Table 3 The impact of animal feed of the present invention on weaned piglets

[0034]

[0035] As can be seen from Table 3: compared with the blank control group, the daily gain of weaned piglets in the test group fed the feed of the present invention increased by 23.63%, the feed-to-meat ratio decreased by 12.24%, the diarrhea rate decreased by 73.5%, and the survival rate increased by 4.34%.

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Abstract

The invention relates to the technical field of microbial fermentation, and discloses a method for preparing a feed probiotic agent from rice flour wastewater and passion fruit peel by lactic acid bacteria. The used rice flour wastewater is waste generated in the rice flour production process, and the passion fruit peel is waste generated in the food processing process. The processing method comprises the following steps: carrying out physical crushing treatment on the fruit peel to make the particles uniform, producing the feed probiotic agent by using the rice flour wastewater and the passion fruit peel, carrying out a feeding test on piglets by using the produced feed probiotic agent, and the viable count of lactic acid bacteria in the feed reaching 4.3 * 10 < 8 > cfu / mL. Compared with other methods for preparing feed probiotics, the method for preparing the feed probiotics from the rice flour wastewater and the passion fruit peels has the advantages of low economic cost, high production efficiency, convenience in use, short production period and the like, and can be used for feeding piglets, so that the food digestibility of pigs is improved, the feed probiotics are more beneficial to intestinal absorption of livestock and poultry, the intestinal environment of livestock and poultry is improved, and the obvious effect of promoting animal growth is achieved.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for preparing probiotics for feeding by lactic acid bacteria using rice flour wastewater and passion fruit peel. Background technique [0002] Probiotics refer to organisms that maintain the balance of intestinal microorganisms, and are beneficial microorganisms that exist in nature. Probiotics instead of antibiotics have been widely valued and applied as veterinary medicine and feed additives. It can be used to replace feeding antibiotics, enhance immunity, promote animal health and growth, improve feeding efficiency, and reduce feeding costs. Using it instead of feeding antibiotics can reduce antibiotic residues in animal products and inhibit drug-resistant pathogenic bacteria. Guangxi is a major producer of rice noodles and passion fruit, and produces a large amount of rice noodle wastewater and passion fruit peels every year. According to incomplete ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 郝宁欧阳平凯周志强李丛苏智兴韦朝英林海鹏卢运琨
Owner 南宁市拜欧生物工程有限责任公司
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