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Plasma preservation solution and its application

A technology for preserving liquid and plasma, which can be used in applications, preservation of human or animal bodies, animal husbandry, etc., and can solve problems such as no plasma quality control, changes in composition properties, melting, etc.

Active Publication Date: 2021-12-07
TIANJIN DEXIANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is inconvenient to use it as a quality control product for blood type reverse typing. For example, it needs to be thawed before each use, and repeated freezing and thawing may cause changes in the properties of its components.
[0004] At present, there is no plasma quality control product that can be stored stably for a long time at ordinary refrigeration temperature

Method used

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  • Plasma preservation solution and its application
  • Plasma preservation solution and its application
  • Plasma preservation solution and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Preparation of plasma preservation solution

[0023] Prepare 5 groups of plasma preservation solutions with water for injection according to the following ingredients.

[0024] (1) Experimental group 1: glucose 5g / L, citric acid 0.55g / L, sodium citrate 8g / L, sodium chloride 1.6g / L, disodium hydrogen phosphate 0.56g / L, sodium dihydrogen phosphate 0.5 g / L, bovine serum albumin 10g / L, mannitol 10g / L, trehalose 10g / L, sodium azide 1g / L and β-cyclodextrin 1mg / L.

[0025] (2) Experimental group 2: Change the concentration of β-cyclodextrin to 10mg / L, and the concentration of other components in experimental group 1.

[0026] (3) Experimental group 3: Change the concentration of β-cyclodextrin to 50mg / L, and the concentration of other components in experimental group 1.

[0027] (4) Experimental group 4: Change the concentration of β-cyclodextrin to 100mg / L, and the concentration of other components in experimental group 1.

[0028] (5) Experimental group 5: Chang...

Embodiment 2AB

[0029] The preparation of embodiment 2ABO blood type antitype plasma quality control product

[0030] Aspirate 9 mL of the plasma (that is, the supernatant after centrifugation) that has been processed by the aforementioned plasma processing method and add it to blood collection tubes containing 1 mL of the plasma preservation solution prepared in Example 1 without anticoagulants to obtain various plasma preservation solutions. Prepared ABO blood group reverse type plasma quality control. For each plasma preservation solution, prepare two tubes of ABO blood type reverse plasma quality control products, hereinafter referred to as "quality control products", one of which is used for testing, and the other tube is used for static observation. Store in refrigerator at 2-8°C.

[0031] For the convenience of illustration, the plasma quality control products prepared in the preservation solution of Experimental Group 1-5 in Example 1 are correspondingly marked as "#1" to "#5" herein...

Embodiment 3

[0032] Example 3 Specificity and antibody titer detection of each plasma quality control product

[0033] The test tube method was used to detect the specificity and antibody titer of each plasma quality control product, and the reference was the "Clinical Blood Transfusion Test Operation Procedures". The specificity and antibody titer of the A-type quality controls stored in each preservation solution were determined, and compared with the A-type serum quality controls in the commercially available ABO and RhD blood group detection quality controls. The commercially available ABO and RhD blood type test quality control products were purchased from Changchun Boxun Biotechnology Co., Ltd., the specification is: 6 bottles / box (article number: BX7001), and the label indicates 2-8°C storage, and the validity period is 90 days .

[0034] The specific detection method is briefly described as follows. Take 6 test tubes and mark them as 1, 2, 3, 4, 5 and the commercially available c...

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Abstract

This paper discloses a plasma preservation solution, which includes glucose 5g / L, citric acid 0.55g / L, sodium citrate 8g / L, sodium chloride 1.6g / L, disodium hydrogen phosphate 0.56g / L, phosphoric acid Sodium dihydrogen 0.5g / L, bovine serum albumin 10g / L, mannitol 10g / L, trehalose 10g / L, sodium azide 1g / L and β-cyclodextrin 10 to 100mg / L. Using the plasma preservation solution provided in this article, plasma can be stored at 2-8°C for a long time, which is convenient for use as a plasma quality control product in ABO blood group reverse typing test to confirm the accuracy of the test results of ABO blood group reverse typing blood group identification.

Description

technical field [0001] This article relates to plasma preservation solutions, in particular plasma preservation solutions that prolong the shelf life of plasma at refrigerated temperatures. This paper also relates to the application of the plasma preservation solution. Background technique [0002] Quality control is used to test the analytical performance of the detection method, judge the quality of the entire test result based on the results of the quality control product, and prompt or warn the operator of possible problems in the test reagent or test system. Appendix 5.1.4 Quality Control Requirements of "Blood Bank Technical Operation Procedures (2019 Edition)" issued by the National Health and Health Commission: "Effective quality control procedures should be established. Ensure that the quality control substances of known antigens and known antibodies in routine parallel tests The response is correct, otherwise the test must be repeated". Blood transfusion compatib...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02G01N33/96G01N33/80
CPCA01N1/021G01N33/80G01N33/96
Inventor 商巧娜王丽王秀柱黄志刚王伟权
Owner TIANJIN DEXIANG BIOTECHNOLOGY CO LTD
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