Cytokine activity stabilizer and preparation method thereof

An activity stabilizer and cytokine technology, applied in the direction of cytokines/lymphokines/interferons, chemical instruments and methods, specific peptides, etc., can solve the problem of low survival rate of cytokines, inability to store cytokines stably for a long time, and cumbersome production process and other problems, to achieve the effect of simple manufacturing process, preventing degradation and loss of activity, and ensuring integrity

Inactive Publication Date: 2021-08-24
南京艾拓生命科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing cytokine activity stabilizer has a cumbersome production process, and the survival rate of cytokine preservation is low, and the cytokine cannot be stored stably for a long time. To solve these problems, here we propose a cytokine activity stabilizer and its preparation method

Method used

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  • Cytokine activity stabilizer and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Disodium cromolyn 4g, ecodoine 1.8g, chitosan 7g, diethylamine tetraacetic acid potassium salt 6g, glucosamine hydrochloride 8g, vitamin C 1g, physiological salt buffer 6g, allantoin 6g, grass Salt 2.8g, glycine-sodium hydroxide-hydrochloric acid buffer 2.2g, carmellose 1.8g, protease inhibitor 1.5g, phosphatase inhibitor 3g, anticoagulant 3g, distilled water 12g.

[0034] Earlier disodium cromolyn 4g, ecodoine 1.8g, chitosan 7g, diethylamine tetraacetic acid potassium salt 6g, glucosamine hydrochloride 8g, vitamin C 1g, concentration 43% physiological saline buffer solution (NaCl, KCl, GaCl 2 and KH 2 PO 4 , dissolved in distilled water, 1:1.6:0.8:0.3 mixed) 6g, add half the amount of 6g distilled water, and then perform ultrasonic vibration, the vibration time is controlled at 24h;

[0035] Then allantoin 6g, oxalate 2.8g, glycine-sodium hydroxide-hydrochloric acid buffer 2.2g, carmellose 1.8g, protease inhibitor 1.5g, phosphatase inhibitor 3g, anticoagulant (hirud...

Embodiment 2

[0037] Disodium cromolyn 2g, ecodoine 1g, chitosan 4g, diethylaminotetraacetic acid potassium salt 3.5g, glucosamine hydrochloride 3g, vitamin C 0.3g, physiological salt buffer 2g, allantoin 1.5g , oxalate 1.4g, glycine-sodium hydroxide-hydrochloric acid buffer 1.6g, carmellose 0.8g, protease inhibitor 0.6g, phosphatase inhibitor 1.7g, anticoagulant 1.1g, distilled water 8g.

[0038]Disodium cromolyn 2g, ecodoine 1g, chitosan 4g, diethylamine tetraacetic acid potassium salt 3.5g, glucosamine hydrochloride 3g, vitamin C 0.3g, concentration 24% physiological saline buffer (NaCl , KCl, GaCl 2 and KH 2 PO 4 , dissolved in distilled water, 1:1.6:0.8:0.3 mixed) 6g, add half the amount of 4g distilled water, and then perform ultrasonic vibration, the vibration time is controlled at 24h;

[0039] Then allantoin 1.5g, oxalate 1.4g, glycine-sodium hydroxide-hydrochloric acid buffer 1.6g, carmellose 0.8g, protease inhibitor 0.6g, phosphatase inhibitor 1.7g, anticoagulant ( Sodium cit...

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Abstract

The invention discloses a cytokine activity stabilizer. The cytokine activity stabilizer is prepared from the following components in parts by weight: 2 to 4 parts of disodium cromoglycin, 1 to 1.8 parts of ecodoine, 4 to 7 parts of chitosan, 3.5 to 6 parts of diethylamine tetraacetic acid potassium salt, 3 to 8 parts of glucosamine hydrochloride, 0.3 to 1 part of vitamin C, 2 to 6 parts of physiological salt buffer solution, 1.5 to 6 parts of allantoin, 1.4 to 2.8 parts of oxalate, 1.6 to 2.2 parts of glycine-sodium hydroxide-hydrochloric acid buffer solution, 0.8 to 1.8 parts of carboxymethyl cellulose, 0.6 to 1.5 parts of protease inhibitor, 1.7-3 parts of a phosphatase inhibitor, 1.1-3 parts of an anticoagulant and 8-12 parts of distilled water. The stabilizer disclosed by the invention can be used for stably storing the cytokines for a long time, so that the integrity of the cytokines is ensured, the degradation and inactivation of the cytokines are prevented, and the whole preparation process is relatively simple.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a cytokine activity stabilizer and a preparation method thereof. Background technique [0002] Cytokines are synthesized and secreted by immune cells (such as monocytes, macrophages, T cells, B cells, NK cells, etc.) and certain non-immune cells (endothelial cells, epidermal cells, fibroblasts, etc.) A class of small molecular proteins with a wide range of biological activities. Cytokines generally regulate cell growth, differentiation and effects by binding to corresponding receptors, and regulate immune responses. Cytokine (CK) is a low-molecular-weight soluble protein that is induced by immunogens, mitogens or other stimulators, and has the ability to regulate innate and adaptive immunity, hematopoiesis, cell growth, APSC pluripotent cells and injury tissue repair and many other functions. Cytokines can be classified into interleukins, interferons, tumor necrosis factor superfa...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C07K14/52
CPCC07K14/52
Inventor夏智敏李志远范元志
Owner南京艾拓生命科技有限公司