Medicament for preventing and treating black spot of passion fruits and preparation method thereof

A technology for black spot disease and passion fruit is applied in the field of pharmaceuticals for preventing and treating passion fruit black spot disease and the field of preparation thereof, which can solve problems such as harm to human health, poor efficacy, economic loss, etc. Spot disease, the effect of improving safety

Pending Publication Date: 2021-09-03
GUANGXI SUBTROPICAL CROPS RES INST GUANGXI SUBTROPICAL AGRI PROD PROCESSING RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The diseased fruit eventually shrinks and most of them fall off, and most of the diseased leaves also dry up and fall off, which not only affects the taste of the fruit, but also makes the fruit ugly and difficult to sell; it even affects the storage and transportation of the fruit after picking, causing great economic losses
[0003] At present, the main measures for the prevention and treatment of passion fruit black spot are to use drugs from the young fruit stage to the mature stage, most of which are chemical agents, but frequent use of chemical agents is likely to make pathogenic bacteria resistant to drugs, resulting in worsening efficacy; in addition, chemical agents are not easy to Decompose and remain on the surface of the soil and fruit, causing the pesticide residue on passion fruit products to exceed the standard seriously, endangering human health and causing environmental pollution

Method used

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  • Medicament for preventing and treating black spot of passion fruits and preparation method thereof
  • Medicament for preventing and treating black spot of passion fruits and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] A medicament for preventing and treating black spot of passion fruit consists of the following components: chitosan, sodium alginate, marine yeast R. paludigenum, arginine and glycerin.

[0022] The preparation method of described control passion fruit black spot medicament, comprises the following steps:

[0023] (1) Weigh 10 parts of chitosan and dissolve in 1% (v / v) acetic acid, add 5 parts of sodium alginate and 8 parts of glycerin, stir and mix at 80°C for 1 hour, put it into an ultrasonic cleaner for defoaming , to obtain membrane solution;

[0024] (2) Activate the marine yeast R.paludigenum in NYDA slant medium, place it in an incubator for 48 hours at 28°C after streak inoculation, and repeat passage twice to obtain activated yeast; the activated yeast was inoculated with The loop was inserted into the NYDB seed medium, and cultured at 200rpm and 28°C for 24h to a concentration of 1×10 8 cells / mL, to obtain the bacterial liquid;

[0025] The components of th...

Embodiment 2

[0030] A medicament for preventing and treating black spot of passion fruit consists of the following components: chitosan, sodium alginate, marine yeast R. paludigenum, arginine and glycerin.

[0031] The preparation method of described control passion fruit black spot medicament, comprises the following steps:

[0032] (1) Weigh 14 parts of chitosan and dissolve in 1% (v / v) acetic acid, add 7 parts of sodium alginate and 10 parts of glycerin, stir and mix at 80°C for 1 hour, put it into an ultrasonic cleaner for defoaming , to obtain membrane solution;

[0033] (2) Activate the marine yeast R.paludigenum in NYDA slant medium, place it in an incubator for 48 hours at 28°C after streak inoculation, and repeat passage twice to obtain activated yeast; the activated yeast was inoculated with The loop was inserted into the NYDB seed medium, and cultured at 200rpm and 28°C for 24h to a concentration of 1×10 8 cells / mL, to obtain the bacterial liquid;

[0034] The components of t...

Embodiment 3

[0039] A medicament for preventing and treating black spot of passion fruit consists of the following components: chitosan, sodium alginate, marine yeast R. paludigenum, arginine and glycerin.

[0040] The preparation method of described control passion fruit black spot medicament, comprises the following steps:

[0041] (1) Weigh 12 parts of chitosan and dissolve in 1% (v / v) acetic acid, add 6 parts of sodium alginate and 9 parts of glycerin, stir and mix at 80°C for 1 hour, put it into an ultrasonic cleaner for defoaming , to obtain membrane solution;

[0042] (2) Activate the marine yeast R.paludigenum in NYDA slant medium, place it in an incubator for 48 hours at 28°C after streak inoculation, and repeat passage twice to obtain activated yeast; the activated yeast was inoculated with The loop was inserted into the NYDB seed medium, and cultured at 200rpm and 28°C for 24h to a concentration of 1×10 8 cells / mL, to obtain the bacterial liquid;

[0043] The components of th...

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Abstract

The invention discloses a medicament for preventing and treating black spot of passion fruits and a preparation method thereof, the medicament is prepared from chitosan, sodium alginate, marine yeast R.pududienum, arginine and glycerol, the medicament is a coating liquid, and the surface of the passion fruit is coated with the coating liquid when the medicament is used. The medicament is prepared from natural products, no residue is generated to cause pollution, no harm is generated to a human body, meanwhile, the marine yeast R.pududienum is added to inhibit growth of pathogenic bacteria alternaria alternata, arginine is added to promote the activity of antagonistic yeast for secreting chitinase, and therefore the inhibiting effect of the medicament on pathogenic bacteria is enhanced, and the black spot of the passion fruits can be better prevented and treated.

Description

technical field [0001] The invention belongs to the technical field of fruit preservation, in particular to a medicament for preventing and treating passion fruit black spot and a preparation method thereof. Background technique [0002] Passion fruit is sweet and sour, with strong flavor and pleasant aroma. It is very popular with consumers when it is made into fruit juice or beverage. Passion fruit black spot, also known as brown spot, mainly damages fruits and leaves. The fruit is diseased, with dark green, water-soaked lesions at first, and then expanding into round, nearly round, or irregular slightly sunken lesions, and the lesions are dark brown or brown. The diseased fruit eventually shrinks, most of which fall off, and most of the diseased leaves also dry up and fall off, which not only affects the taste of the fruit, but also makes the fruit look ugly and difficult to sell; it even affects the storage and transportation of the fruit after picking, causing great ec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A01G13/00C12R1/645
CPCC12N1/16A01G13/00
Inventor 曾黎明李穆巫辅民李季东蔡元保庞新华谢朝敏黄思婕潘如军郑文武李冰叶雪英雷刚志林玉虹黄锦媛叶维雁
Owner GUANGXI SUBTROPICAL CROPS RES INST GUANGXI SUBTROPICAL AGRI PROD PROCESSING RES INST
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