Endangered plant sphaeropteris lepifera propagation technology and field regression method
A pen holder and technology are applied in the field of breeding methods and special equipment for endangered plant pen holder trees, which can solve the problems of lack of comprehensive and systematic research, influence on growth, and difficulty in forming a culture system, and achieve growth and physiological resistance indicators. Improves promotion, reduces algal pollution and weeds, and shortens the vegetative cycle
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Embodiment 1
[0062] A kind of pen holder tree propagation technology, specifically comprises the steps:
[0063] (1) Disinfection treatment: use fresh spores as the propagation material, soak the collected mature spores in sterile water for 24 hours, filter, then put the spores into a 1.5ml centrifuge tube, add 1ml sterile water, put them into a centrifuge, 2500r / Centrifuge at a speed of 3min for 3min, remove the supernatant, add 1ml of a solution mixed with 15% sodium hypochlorite and 10% hydrogen peroxide solution in a volume ratio of 3:1 into the centrifuge tube again, let it stand for sterilization for 4min, and rinse the pen holder tree with sterile water Spore 3 times, obtain aseptic pen holder tree spore suspension;
[0064] (2) Spore inoculation: inoculate the sterilized spore suspension onto the surface of the germination medium, the germination medium: the lower layer of absorbent cotton, put 1 / 4MS liquid medium in the middle, and the filter paper containing sterile water in the...
Embodiment 2
[0069] A pen container tree transplanting technique, wherein, the transplanting substrate: peat, perlite and vermiculite, the volume ratio is 3:1:1, the organic matter content is 275.60g / kg, the available phosphorus content is 0.052g / kg, and the available potassium The content is 0.23g / kg, the available nitrogen content is 0.35g / kg, and the bulk density is 0.23g / cm 3 , the maximum water holding capacity is 373.7%;
[0070] Management measures after transplanting: After growing for 2-3 months under 75% shade, accept normal natural light for growth.
Embodiment 3
[0072] A pen container tree transplanting technique and field return planting method, wherein, the transplanting substrate: peat, perlite and vermiculite, the volume ratio is 3:1:1, the organic matter content is 275.60g / kg, and the available phosphorus content is 0.052g / kg, the available potassium content is 0.23g / kg, the available nitrogen content is 0.35g / kg, and the bulk density is 0.23g / cm 3 , the maximum water holding capacity is 373.7%;
[0073] Management measures after transplanting: After growing for 2-3 months under 75% shading, accept normal natural light for growth, and use the high-temperature protection device for endangered plant cultivation of the present invention in conjunction with shading.
[0074] When the pen container tree grows to 1-2 years old, return to the field for planting, set up a temperature, humidity and light monitor, and regularly monitor and record the environmental conditions and growth and development of the seedlings after returning to t...
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