Endangered plant sphaeropteris lepifera propagation technology and field regression method

A pen holder and technology are applied in the field of breeding methods and special equipment for endangered plant pen holder trees, which can solve the problems of lack of comprehensive and systematic research, influence on growth, and difficulty in forming a culture system, and achieve growth and physiological resistance indicators. Improves promotion, reduces algal pollution and weeds, and shortens the vegetative cycle

Active Publication Date: 2021-09-07
ZHEJIANG SUB TROPICS CROP INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method undergoes long-term tissue culture, and explant disinfection can easily pollute the pen holder tree and affect its vitality. The survival rate of this culture method is low, and it is impossible to form a large number of propagation systems.
In fact, we found that the tissue culture and reproduction of the leaves of the pen container tree and other parts of the leaves encountered bottlenecks, serious pollution, and the problem of long-term "pollution" cannot be overcome, and it is not easy to form a stable culture system
[0006] At the same time, the growth of the pen container tree after transplanting is also greatly affected by the environment. Too high or too low temperature will affect its growth. At present, only the breeding method of the pen container tree has been studied. There is a lack of comprehensive and systematic research on the measures applicable to the protection of endangered plants

Method used

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  • Endangered plant sphaeropteris lepifera propagation technology and field regression method
  • Endangered plant sphaeropteris lepifera propagation technology and field regression method
  • Endangered plant sphaeropteris lepifera propagation technology and field regression method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] A kind of pen holder tree propagation technology, specifically comprises the steps:

[0063] (1) Disinfection treatment: use fresh spores as the propagation material, soak the collected mature spores in sterile water for 24 hours, filter, then put the spores into a 1.5ml centrifuge tube, add 1ml sterile water, put them into a centrifuge, 2500r / Centrifuge at a speed of 3min for 3min, remove the supernatant, add 1ml of a solution mixed with 15% sodium hypochlorite and 10% hydrogen peroxide solution in a volume ratio of 3:1 into the centrifuge tube again, let it stand for sterilization for 4min, and rinse the pen holder tree with sterile water Spore 3 times, obtain aseptic pen holder tree spore suspension;

[0064] (2) Spore inoculation: inoculate the sterilized spore suspension onto the surface of the germination medium, the germination medium: the lower layer of absorbent cotton, put 1 / 4MS liquid medium in the middle, and the filter paper containing sterile water in the...

Embodiment 2

[0069] A pen container tree transplanting technique, wherein, the transplanting substrate: peat, perlite and vermiculite, the volume ratio is 3:1:1, the organic matter content is 275.60g / kg, the available phosphorus content is 0.052g / kg, and the available potassium The content is 0.23g / kg, the available nitrogen content is 0.35g / kg, and the bulk density is 0.23g / cm 3 , the maximum water holding capacity is 373.7%;

[0070] Management measures after transplanting: After growing for 2-3 months under 75% shade, accept normal natural light for growth.

Embodiment 3

[0072] A pen container tree transplanting technique and field return planting method, wherein, the transplanting substrate: peat, perlite and vermiculite, the volume ratio is 3:1:1, the organic matter content is 275.60g / kg, and the available phosphorus content is 0.052g / kg, the available potassium content is 0.23g / kg, the available nitrogen content is 0.35g / kg, and the bulk density is 0.23g / cm 3 , the maximum water holding capacity is 373.7%;

[0073] Management measures after transplanting: After growing for 2-3 months under 75% shading, accept normal natural light for growth, and use the high-temperature protection device for endangered plant cultivation of the present invention in conjunction with shading.

[0074] When the pen container tree grows to 1-2 years old, return to the field for planting, set up a temperature, humidity and light monitor, and regularly monitor and record the environmental conditions and growth and development of the seedlings after returning to t...

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PUM

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Abstract

The invention relates to an endangered plant sphaeropteris lepifera propagation technology and a field regression method. The technical problems that spore propagation of sphaeropteris lepifera is difficult, and the requirements of spores for matrix water-retaining property, air permeability and nutrient supply are harsh are solved. A disinfection scheme is continuously optimized for a long time, meanwhile, a culture medium is changed, sterile filter paper is explored to be placed in a culture dish, the growth conditions of the spores of the sphaeropteris lepifera are regulated and controlled, and the breeding effect of accelerating the spores to the gametophyte period is achieved. Meanwhile, through precise control after transplanting and matching with special equipment, a stable breeding system of the sphaeropteris lepifera is formed, and the technical problem of sphaeropteris lepifera breeding is effectively solved.

Description

technical field [0001] The application relates to the field of plant protection, in particular to a breeding method and special equipment applied to the endangered plant pen holder tree. Background technique [0002] Endangered plants refer to plants that are facing the crisis of extinction due to changes in the environment. According to plant researchers, the extinction of species and the formation of new species are a natural evolutionary process. Due to changes in the earth's environment, some species cannot adapt to the new environment. While extinct, some new species adapting to the new environment are formed at the same time. The survival of plants, as an indispensable link in biodiversity and ecosystems, has been subject to the pressure of internal and external troubles, and a large number of plants are going to be endangered silently. The reasons for the endangerment of plants are very complex, but external factors such as improper human activities, climate change an...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00A01G17/00A01G7/04A01G24/28A01G24/15A01G13/02
CPCA01H4/008A01H4/002A01H4/00A01G31/00A01G17/005A01G7/045A01G24/28A01G24/15A01G13/0212Y02A40/22
Inventor 胡青荻马晓华钱仁卷张旭乐郑坚李其佐魏君艳汤真勇陈义增陈旭华
Owner ZHEJIANG SUB TROPICS CROP INST
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