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A kind of pnipaam(am)/da composite imprinting gel and its preparation method and application

A technology of imprinting gel and lysozyme, which is applied in the direction of biochemical equipment and methods, pharmaceutical formulations, medical preparations of non-active ingredients, etc., can solve problems such as collapse, damage, and performance degradation, and achieve uniform gel and good mechanics performance effect

Active Publication Date: 2022-05-10
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although PNIPAAm has the characteristic of temperature response, its LCST is close to body temperature (Cho EC, Kim DH, ChoK. Contact angles of oils on solid substrates in aqueous media: Correlation with AFM data on protein adhesion, Langmuir.2008; 24:9974-8 ), but it is often prepared into a temperature-sensitive system and after swelling, due to its own notch sensitivity, it is prone to permanent collapse and damage, which eventually leads to a decline in its controlled release performance

Method used

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  • A kind of pnipaam(am)/da composite imprinting gel and its preparation method and application
  • A kind of pnipaam(am)/da composite imprinting gel and its preparation method and application
  • A kind of pnipaam(am)/da composite imprinting gel and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Preparation of PNIPAAm(AM) / DA composite imprinted gel

[0046] (1) 1.5 mg of dopamine (purchased from Sigma) was dissolved in 5 ml of an aqueous solution with pH=8.5, and then the dopamine solution was stirred and oxidized in air for 0.5 h to form a polydopamine solution.

[0047] (2) Dissolve 301.18mg of N-isopropylacrylamide, 26.19mg of acrylamide, 18mg of N,N-methylenebisacrylamide, and 10mg of lysozyme in the polydopamine solution, and then seal it and stir it with nitrogen gas for 0.5h. After exhausting the oxygen in the solution with nitrogen, add 20 mg of initiator ammonium persulfate, and then pass nitrogen again for 15 minutes. After the nitrogen is passed, a prepolymer solution is formed; finally add 20 μL N,N,N',N' to the solution -Tetramethylethylenediamine, the initial product of PNIPAAm(AM) / DA composite imprinted gel is prepared; quickly use the pipette gun to move the gel solution between the two cleaned slides, and after 6 hours of reaction, p...

Embodiment 2

[0048] Example 2: Preparation of PNIPAAm(AM) / DA composite imprinted gel

[0049] (1) 2.0 mg of dopamine (purchased from Sigma) was dissolved in 5 ml of an aqueous solution with pH=8.5, and then the dopamine solution was stirred and oxidized in air for 0.5 h to form a polydopamine solution.

[0050] (2) Dissolve 301.18mg of N-isopropylacrylamide, 26.19mg of acrylamide, 18mg of N,N-methylenebisacrylamide, and 10mg of lysozyme in the polydopamine solution, and then seal it and stir it with nitrogen gas for 0.5h. After exhausting the oxygen in the solution with nitrogen, add 20 mg of initiator ammonium persulfate, and then pass nitrogen again for 15 minutes. After the nitrogen is passed, a prepolymer solution is formed; finally add 20 μL N,N,N',N' to the solution -Tetramethylethylenediamine, the initial product of PNIPAAm(AM) / DA composite imprinted gel is prepared; quickly use the pipette gun to move the gel solution between the two cleaned slides, and after 6 hours of reaction, p...

Embodiment 3

[0051] Example 3: Preparation of PNIPAAm(AM) / DA composite imprinted gel

[0052] (1) 2.5 mg of dopamine (purchased from Sigma) was dissolved in 5 ml of an aqueous solution with pH=8.5, and then the dopamine solution was stirred and oxidized in air for 0.5 h to form a polydopamine solution.

[0053] (2) Dissolve 301.18mg of N-isopropylacrylamide, 26.19mg of acrylamide, 18mg of N,N-methylenebisacrylamide, and 10mg of lysozyme in the polydopamine solution, and then seal it and stir it with nitrogen gas for 0.5h. After exhausting the oxygen in the solution with nitrogen gas, a pre-polymerization solution is formed; add 20 mg of initiator ammonium persulfate, and then pass nitrogen gas again for 15 minutes, and finally add 20 μL N,N,N',N' to the solution -Tetramethylethylenediamine, the initial product of PNIPAAm(AM) / DA composite imprinted gel is prepared; quickly use the pipette gun to move the gel solution between the two cleaned slides, and after 6 hours of reaction, place the u...

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PUM

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Abstract

The invention belongs to the field of biological functional materials, and discloses a PNIPAAm(AM) / DA composite imprinted gel, a preparation method and application thereof. The preparation method introduces polydopamine (PDA) into thermosensitive poly-N-isopropylacrylamide (PNIPAAm) and acrylamide (AM) composite gel by free radical polymerization, and prepares a new type of PNIPAAm (AM) / DA composite imprinted gel, because PDA was introduced into the original PNIPAAm(AM) imprinted layer, the strength of the new composite imprinted gel was greatly improved while maintaining the temperature-sensitive properties of PNIPAAm, making the composite imprinted gel The gel avoids the destruction of the three-dimensional imprinted holes on its surface during the time period of temperature-responsive changes, thereby finally improving the temperature-sensitive and controlled release performance of the composite imprinted gel for the template protein.

Description

technical field [0001] The invention belongs to the field of biological functional materials, and in particular relates to a PNIPAAm (AM) / DA composite imprinted gel and a preparation method and application thereof. Background technique [0002] Biological macromolecules play a very important role in our living organisms. The continuous exploration of macromolecules such as peptides and proteins can not only provide a basic scientific basis for explaining the laws of activity of substances in living organisms, but also provide a basis for future research in the field of medicine. The overcoming of intractable diseases provides the necessary basic theoretical basis and solutions. When the biomedical materials we use enter the body and function, the first reaction is the non-specific adhesion of the macromolecular substances present in the blood or body fluids to the surface of the biomaterial, and then due to the type and quantity of the adsorbed protein The difference betwee...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J9/26C08J3/075C08F283/00C08F220/54C08F220/56C08F222/38C12N11/082C12N11/098A61K47/32A61K47/34A61K9/06
CPCC08J9/26C08J3/075C08F283/00C12N11/082C12N11/098C12N9/2462C12Y302/01017A61K47/32A61K47/34A61K9/06C08J2201/0424C08J2351/08C08F220/54C08F220/56C08F222/385
Inventor 柯渔范家琛敖宁建
Owner JINAN UNIVERSITY
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