Application of 61-bp indel polymorphism of chicken RIN2 gene in predicting and identifying of abdominal fat traits of chicken

Abstract

The invention relates to application of a 61-bp indel polymorphism of a NC_052534.1: g.4350067-4350127 site of a chicken RIN2 gene in predicting and identifying of abdominal fat traits of a chicken. The 61-bp indel polymorphism can be used for effectively predicting and identifying the abdominal fat traits of the chicken as a molecular marker, including chicken fat bandwidth, abdominal fat weight and abdominal fat percentage. According to the molecular marker, genetic typing is carried out by adopting a method for detecting a PCR product through agarose gel electrophoresis, the operation is simple and convenient, and the cost is relatively low.

Description

Technical field

[0001] The present invention belongs to the technical field of molecular genetics, particularly relates to a chicken gene RIN2 NC_052534.1: Application g.4350067-4350127 bit 61-bp indel polymorphisms identified in chicken and indicates abdominal fatness traits. Background technique

[0002] Broiler is mankind's most common breeding poultry, with the growth rate of broiler breeders to choose, while improving the performance of broilers, also caused a number of physiological disorders such suited to the problem, such as body fat content is too high, sudden death syndrome, ascites comprehensive psychosis negative effects. Where excessive body fat problem is a problem faced by breeders, excessive deposition of fat in poultry is not their physical needs, most of the fat will be discarded at slaughter time directly, directly affect the quality of meat products, reduce slaughter rate and economic benefits.

[0003] The main factors that affect fat deposition in broilers ...

Examples

Embodiment 1

[0034] Example 1 primer design

[0035] The chicken RIN2 gene NC_052534.1: g.4350067-4350127 site 61-bp indel polymorphism site on the downstream primers were designed RIN2-F and RIN2-R, to obtain amplification primer;

[0036] Primer sequences were designed as follows:

[0037] RIN2-F primer sequence: 5'-AGTCTAGCTCTGGTCGTGTG-3 '

[0038] RIN2-R primer sequence: 5'-CACCGCACCTGATATCCCAC-3 '

[0039] Synthetic engine sequence by the Beijing Branch of the Science and Technology Co., Ltd. Guangzhou Branch.

Embodiment 2

[0040] g.4350067-4350127 bit 61-bp indel polymorphism: Example 2PCR detecting gene NC_052534.1 embodiment Chicken RIN2

[0041] Chicken genomic DNA (Blood DNA Kit D3392, Omega, USA) extracted from chicken blood. The chicken blood from apricot chicken and chicken Recessive White Rock hybridized second generation (F 2 ), A total of 304 individuals. Fly 2 Generation wing individuals venous blood, EDTA-Na2 anticoagulant at 90 days of age. Recessive White Plymouth Rock chicken and apricot chicken blood samples provided by the Key Laboratory of Animal Genomics and Molecular Breeding in Guangdong Province.

[0042] PCR amplification system and the reaction conditions were as follows:

[0043]

[0044] The PCR reaction mixture of the solution: 95 ℃ denaturation 5min, 94 ℃ denaturation 25s, 61 ℃ annealing 25s, 72 ℃ extension 30s, 31 cycles, 72 ℃ extending 5min, 4 ℃ stored PCR product.

[0045] The PCR product was subjected to electrophoresis using 2.0% agarose gel ( figure 1 ), Genotype ...

Embodiment 3

[0054] Correlation Analysis Example 61-bp Indel polymorphism and abdominal fat, abdominal fat weight and fat bandwidth Gene embodiment 3RIN2

[0055] With IBM SPSS Statistics V21.0 software, the following mixed linear models are constructed according to the characteristics of the group:

[0056] ModelI: Y ijklm = Μ + g i + S j + H k + f l + e ijklm3

[0057] Where Y ijklm Is a personalized table value, μ is a population mean, g i Is a log genotype fixed effect, F l Random effect of family, S j Is a gender fixation effect, h k Is a fixed effect of batch, E ijklm It is a random error. Bonferroni 'inspected multiple comparisons, P <0.05 considered significant differences.

[0058] Calculate 61-BP INDEL bit polymorphism and f 2 The correlation between group fat traits, the results of Table 2 show that the Rin261-BP IndEl site polymorphism has a significant effect on abdominal lilation, abdominality and fat bandwidth (P <0.05). Among them, II genotypes are littering rate, abdominal lil...

Technical field

[0001] The present invention belongs to the technical field of molecular genetics, particularly relates to a chicken gene RIN2 NC_052534.1: Application g.4350067-4350127 bit 61-bp indel polymorphisms identified in chicken and indicates abdominal fatness traits. Background technique

[0002] Broiler is mankind's most common breeding poultry, with the growth rate of broiler breeders to choose, while improving the performance of broilers, also caused a number of physiological disorders such suited to the problem, such as body fat content is too high, sudden death syndrome, ascites comprehensive psychosis negative effects. Where excessive body fat problem is a problem faced by breeders, excessive deposition of fat in poultry is not their physical needs, most of the fat will be discarded at slaughter time directly, directly affect the quality of meat products, reduce slaughter rate and economic benefits.

[0003] The main factors that affect fat deposition in broilers ...

Examples

Embodiment 1

[0034] Example 1 primer design

[0035] The chicken RIN2 gene NC_052534.1: g.4350067-4350127 site 61-bp indel polymorphism site on the downstream primers were designed RIN2-F and RIN2-R, to obtain amplification primer;

[0036] Primer sequences were designed as follows:

[0037] RIN2-F primer sequence: 5'-AGTCTAGCTCTGGTCGTGTG-3 '

[0038] RIN2-R primer sequence: 5'-CACCGCACCTGATATCCCAC-3 '

[0039] Synthetic engine sequence by the Beijing Branch of the Science and Technology Co., Ltd. Guangzhou Branch.

Embodiment 2

[0040] g.4350067-4350127 bit 61-bp indel polymorphism: Example 2PCR detecting gene NC_052534.1 embodiment Chicken RIN2

[0041] Chicken genomic DNA (Blood DNA Kit D3392, Omega, USA) extracted from chicken blood. The chicken blood from apricot chicken and chicken Recessive White Rock hybridized second generation (F 2 ), A total of 304 individuals. Fly 2 Generation wing individuals venous blood, EDTA-Na2 anticoagulant at 90 days of age. Recessive White Plymouth Rock chicken and apricot chicken blood samples provided by the Key Laboratory of Animal Genomics and Molecular Breeding in Guangdong Province.

[0042] PCR amplification system and the reaction conditions were as follows:

[0043]

[0044] The PCR reaction mixture of the solution: 95 ℃ denaturation 5min, 94 ℃ denaturation 25s, 61 ℃ annealing 25s, 72 ℃ extension 30s, 31 cycles, 72 ℃ extending 5min, 4 ℃ stored PCR product.

[0045] The PCR product was subjected to electrophoresis using 2.0% agarose gel ( figure 1 ), Genotype ...

Embodiment 3

[0054] Correlation Analysis Example 61-bp Indel polymorphism and abdominal fat, abdominal fat weight and fat bandwidth Gene embodiment 3RIN2

[0055] With IBM SPSS Statistics V21.0 software, the following mixed linear models are constructed according to the characteristics of the group:

[0056] ModelI: Y ijklm = Μ + g i + S j + H k + f l + e ijklm3

[0057] Where Y ijklm Is a personalized table value, μ is a population mean, g i Is a log genotype fixed effect, F l Random effect of family, S j Is a gender fixation effect, h k Is a fixed effect of batch, E ijklm It is a random error. Bonferroni 'inspected multiple comparisons, P <0.05 considered significant differences.

[0058] Calculate 61-BP INDEL bit polymorphism and f 2 The correlation between group fat traits, the results of Table 2 show that the Rin261-BP IndEl site polymorphism has a significant effect on abdominal lilation, abdominality and fat bandwidth (P <0.05). Among them, II genotypes are littering rate, abdominal lil...

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