Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lipid raft@organic-inorganic hybrid monolithic column as well as preparation method and application thereof

A technology of monolithic columns and lipid rafts, which is applied in inorganic chemistry, chemical instruments and methods, alkali metal compounds, etc., and can solve the problems such as the lack of lipid rafts.

Pending Publication Date: 2021-09-14
JIANGSU UNIV
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the organic-inorganic hybrid monolithic column of lipid rafts

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lipid raft@organic-inorganic hybrid monolithic column as well as preparation method and application thereof
  • Lipid raft@organic-inorganic hybrid monolithic column as well as preparation method and application thereof
  • Lipid raft@organic-inorganic hybrid monolithic column as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Preparation of organic-inorganic hybrid monolithic column: mix 0.2mL of silane coupling agent vinyltriethoxysilane, 0.2mL of tetramethoxysilane, and 0.2mL of acetic acid, add organic monomer methyl methacrylate 0.3mL ester, 1mL porogen dodecanol, 0.3mL cross-linking agent ethylene glycol dimethacrylate and 0.02g initiator azobisisobutyronitrile, after ultrasonication for 30 min to remove air bubbles, place in a water bath at 60°C The organic-inorganic hybrid monolithic column was prepared by reacting for 24 hours. The organic-inorganic hybrid monolithic column has normal crosslinkability, a total pore volume of 0.65 ml / g, and moderate hardness. figure 1 is the infrared spectrogram of the prepared organic-inorganic hybrid monolithic column; as figure 1 Visible, 2990 cm- 1 and 3000 cm -1 The peak at is from the C-H of aromatic hydrocarbons or olefins, 1750cm -1 The left and right peaks are -C=O, 1480 cm -1 The left and right peaks are C-O-C groups, 752 cm -1 and...

Embodiment 2

[0033] In this example, TrkA protein lipid rafts@organic-inorganic hybrid monolithic columns with lipid raft perfusion ratios of 1:1, 2:1, and 3:1 were prepared by the same method as in Example 1 (respectively as samples 2~4), and the organic-inorganic hybrid monolithic column was used as the control (sample 1) to collect the lipid raft effluent for western blot experiments, and flotillin-1 was selected as the lipid raft marker protein. Figure 5 It is the graph of western blot experiment results of lipid raft effluent. Such as Figure 5 As shown, all samples ran out bands, the band of sample 1 was clear, and the protein bands of samples 2, 3, and 4 became weaker, indicating that lipid rafts were successfully modified on the organic-inorganic hybrid monolithic column.

[0034] The TrkA protein lipid raft@organic-inorganic hybrid monolithic column solid phase with lipid raft perfusion ratios of 1:1, 2:1, and 3:1 was taken for sample preparation and western blot characterizatio...

Embodiment 3

[0037] In this example, the BET method was used to detect the specific surface area of ​​the TrkA protein lipid raft@organic-inorganic hybrid monolithic column prepared in Example 1. The specific detection conditions are degassing temperature 200 ℃; degassing time 60 min; adsorbate is nitrogen; saturated vapor pressure is 1.0039 bar; constant temperature bath temperature is 77.3 K; ambient temperature is 20 ℃. The specific surface area of ​​TrkA protein lipid raft@organic-inorganic hybrid monolithic column measured by BET multipoint method is 12.4 m 2 / g, the specific surface area of ​​the organic-inorganic hybrid monolithic column is 279.6 m 2 / g. It can be seen that the specific surface area of ​​the organic-inorganic hybrid monolithic column is significantly reduced after TrkA protein lipid raft modification, indicating that the lipid raft has been modified on the organic-inorganic hybrid monolithic column.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Specific surface areaaaaaaaaaaa
Specific surface areaaaaaaaaaaa
Concentrationaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the field of active pharmaceutical ingredient screening, and particularly relates to a lipid raft@organic-inorganic hybrid monolithic column as well as a preparation method and application thereof. According to the invention, an organic-inorganic hybrid monolithic column is used as a matrix, and the permeability and the specific surface area of the organic-inorganic hybrid monolithic column are superior to the permeability and the specific surface area of a traditional silica gel packed column; a lipid raft well modifies the surface of the organic-inorganic hybrid monolithic column; the prepared lipid raft@organic-inorganic hybrid monolithic column has a uniform net structure; and different screening requirements can be met by adjustment of the aperture and permeability of the organic-inorganic hybrid monolithic column and modification with lipid rafts from different sources. The lipid raft@organic-inorganic hybrid monolithic column can be applied as an affinity screening model to lipid raft chromatography stationary phase research and online drug screening research.

Description

technical field [0001] The invention belongs to the field of screening active ingredients of medicines, and specifically relates to a lipid raft@organic-inorganic hybrid monolithic column and its preparation method and application. Background technique [0002] The performance of the chromatographic column largely determines the effect of separation and analysis. The traditional chromatographic column packing is made of numerous unconnected silica gel balls filled into the chromatographic column tube. The widely used chromatographic column packing in the market is generally 3 -5μm, the permeability becomes poor after surface biomodification, which limits the development of bioaffinity chromatography. The monolithic column is a cross-linked rod-shaped monolithic stationary phase with a porous structure prepared in the column tube by sol-gel, in-situ condensation and other methods. It has a large specific surface area, high column efficiency, fast separation speed and column p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): B01J20/285B01J20/28B01J20/30G01N30/60
CPCB01J20/285B01J20/28052B01J20/3064G01N30/60
Inventor 童珊珊田胜
Owner JIANGSU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com