Application of indoleacetic acid in the preparation of drugs for the prevention and treatment of chronic obstructive pulmonary disease
A technology for chronic obstructive pulmonary disease and indole acetic acid, applied in the field of medicine, can solve problems such as unreported effects, and achieve the effects of safe use, reduced ratio, and easy control.
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Embodiment 1
[0029] Example 1: Preparation of chronic obstructive pulmonary disease mouse model and intervention with indole acetic acid
[0030] 1. Preparation of COPD mouse model:
[0031] Male C57BL / 6J mice aged 6-8 weeks, weighing 18-25 g, were purchased from Liaoning Changsheng Biotechnology Co., Ltd. The mice were randomly divided into 4 groups, namely the control group (Control group), the indole acetic acid group (IAA group), the model group (COPD group), and the model plus indole acetic acid group (COPD+IAA group). From the first day of the experiment, 7 μg lipopolysaccharide (LPS) + 1.2U elastase (Elastase) (dissolved in 100 μL PBS) was instilled into the lungs of each mouse in the COPD group and COPD+IAA group by nasal drop , dropped once a week, continuously dropped for 4 weeks, Control group and IAA group dropped PBS in the same way.
[0032] 2. Indole acetic acid intervention:
[0033] Use an appropriate amount of 1mol / L sodium hydroxide to completely dissolve the indole a...
Embodiment 2
[0034] Example 2: Indoleacetic acid improves lung function in a mouse model of chronic obstructive pulmonary disease
[0035] 1. Pulmonary function test:
[0036] The model group and non-model group mice that have completed the model preparation and IAA intervention were tested for lung function, and the mice were anesthetized with tribromoethanol, and the trachea of the mice was exposed with sterilized surgical instruments, and passed through the experimental animal lung function detection system (U.S. DSI Company) detects and reads the mouse lung function index FEV100 and FVC index, and calculates the ratio of FEV100 / FVC. After the pulmonary function test is completed, follow-up tests of other indicators are carried out.
[0037] 2. Experimental results:
[0038] like figure 1 As shown, the COPD group compared with the Control group, the COPD group FEV100 / FVC significantly decreased (p<0.05), and the COPD+IAA group compared with the COPD group, the former FEV100 / FVC signi...
Embodiment 3
[0039] Example 3: Indole acetic acid can inhibit the production of inflammatory factors in the lungs of chronic obstructive pulmonary disease model mice
[0040] 1. Obtain the supernatant of alveolar lavage fluid:
[0041] Alveolar lavage fluid was obtained by alveolar lavage of the mice that had completed the lung function test, a sterilized 20G PE needle was inserted into the exposed trachea, and the PE needle was fixed with a surgical thread. Use a 1 mL sterile syringe to take 0.8 mL of phosphate buffered saline (PBS) pre-cooled on ice, connect the fixed PE needle to lavage the mouse lungs, and gently lavage back and forth three times to obtain alveolar lavage fluid. The alveolar lavage fluid was centrifuged at 5000g and 4°C for 10 minutes, and the supernatant was transferred to a new centrifuge tube for subsequent detection. The cell pellet was reserved for alveolar lavage fluid smear and HE staining.
[0042] 2. ELISA detection of inflammatory factors:
[0043] ELISA ki...
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