Fluorescence intensity detection method and device for porous fluorescent microarray image, computer equipment and computer readable storage medium

Abstract

The invention relates to the technical field of digital image processing, and discloses a fluorescence intensity detection method and device for a porous fluorescent microarray image, computer equipment and a computer readable storage medium. After a porous fluorescent microarray image is subjected to filtering and denoising processing, image binarization processing and morphological processing in sequence, a binarized image which is easy to perform contour extraction accurately can be obtained, and for a boundary contour extracted from the binarized image, determination of a bounding box, gray value traversal calculation of pixel points in the box and final background removal calculation of an average gray value are performed in sequence, so that the algorithm operation speed can be improved, the accuracy of the finally obtained fluorescence intensity detection result can be ensured, the fluorescence intensity data of each single-hole fluorescence region object can be quickly and accurately obtained, the purposes of real-time detection and quantitative analysis can be realized, and the method is convenient for practical application and popularization.

Description

technical field [0001] The invention belongs to the technical field of digital image processing, and in particular relates to a method, a device, a computer device and a computer-readable storage medium for detecting the fluorescence intensity of a porous fluorescent microarray image. Background technique [0002] At the end of the 20th century, Vogelstein et al. first proposed the concept of "digital PCR (digital PCR, dPCR)", which achieved a breakthrough in the absolute quantification of nucleic acid copy number. The main principle of digital PCR is to place a single DNA (DeoxyriboNucleic Acid, deoxyribonucleic acid) molecule in an independent reaction chamber, and perform PCR amplification on it, and use chemical reagents and dye-labeled probes to detect specific target sequences. The ratio and number of reaction units of the two signal types are statistically calculated to achieve absolute quantification of the sample. Therefore, digital PCR is also called single-molecul...

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Problems solved by technology

[0005] In order to solve the problems that the existing image fluorescence intensity detection method detects the fluorescence signal intensity of the porous fluorescent microarray image, the detection speed is slow and the accuracy is not high, the present invention The purpose is to provide a new fluorescence intensity detection method, device, computer equipment and computer-readable storage medium suitable for porous fluorescence microarray images, which can not only improve the running speed of the algorithm, but also ensure the accuracy of the final fluorescence intensity detection results In this way, the fluorescence intensity data of objects in each single-hole fluorescence area can be quickly and accurately obtained, which can realize the purpose of real-time detection and quantitative analysis, and is convenient for practical application and promotion

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