Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fluorescence intensity detection method and device for porous fluorescent microarray image, computer equipment and computer readable storage medium

A technology of fluorescence intensity and detection method, applied in image enhancement, calculation, image analysis, etc., can solve problems such as low accuracy and slow detection speed

Active Publication Date: 2021-09-28
维柯基科技上海有限公司
View PDF6 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems that the existing image fluorescence intensity detection method detects the fluorescence signal intensity of the porous fluorescent microarray image, the detection speed is slow and the accuracy is not high, the present invention The purpose is to provide a new fluorescence intensity detection method, device, computer equipment and computer-readable storage medium suitable for porous fluorescence microarray images, which can not only improve the running speed of the algorithm, but also ensure the accuracy of the final fluorescence intensity detection results In this way, the fluorescence intensity data of objects in each single-hole fluorescence area can be quickly and accurately obtained, which can realize the purpose of real-time detection and quantitative analysis, and is convenient for practical application and promotion

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescence intensity detection method and device for porous fluorescent microarray image, computer equipment and computer readable storage medium
  • Fluorescence intensity detection method and device for porous fluorescent microarray image, computer equipment and computer readable storage medium
  • Fluorescence intensity detection method and device for porous fluorescent microarray image, computer equipment and computer readable storage medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0050] The present invention will be further elaborated below in conjunction with the accompanying drawings and specific embodiments. It should be noted here that although the descriptions of these embodiments are used to help the understanding of the present invention, they are not intended to limit the present invention. Specific structural and functional details disclosed herein are merely for purposes of describing example embodiments of the present invention. However, the invention may be embodied in many alternative forms and should not be construed as limited to the embodiments set forth herein.

[0051] It will be understood that, although the terms first, second, etc. may be used herein to describe various objects, these objects should not be limited by these terms. These terms are only used to distinguish one object from another. For example, a first object could be termed a second object, and, similarly, a second object could be termed a first object, without depa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of digital image processing, and discloses a fluorescence intensity detection method and device for a porous fluorescent microarray image, computer equipment and a computer readable storage medium. After a porous fluorescent microarray image is subjected to filtering and denoising processing, image binarization processing and morphological processing in sequence, a binarized image which is easy to perform contour extraction accurately can be obtained, and for a boundary contour extracted from the binarized image, determination of a bounding box, gray value traversal calculation of pixel points in the box and final background removal calculation of an average gray value are performed in sequence, so that the algorithm operation speed can be improved, the accuracy of the finally obtained fluorescence intensity detection result can be ensured, the fluorescence intensity data of each single-hole fluorescence region object can be quickly and accurately obtained, the purposes of real-time detection and quantitative analysis can be realized, and the method is convenient for practical application and popularization.

Description

technical field [0001] The invention belongs to the technical field of digital image processing, and in particular relates to a method, a device, a computer device and a computer-readable storage medium for detecting the fluorescence intensity of a porous fluorescent microarray image. Background technique [0002] At the end of the 20th century, Vogelstein et al. first proposed the concept of "digital PCR (digital PCR, dPCR)", which achieved a breakthrough in the absolute quantification of nucleic acid copy number. The main principle of digital PCR is to place a single DNA (DeoxyriboNucleic Acid, deoxyribonucleic acid) molecule in an independent reaction chamber, and perform PCR amplification on it, and use chemical reagents and dye-labeled probes to detect specific target sequences. The ratio and number of reaction units of the two signal types are statistically calculated to achieve absolute quantification of the sample. Therefore, digital PCR is also called single-molecul...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G06T5/00G06T7/00G06T7/11G06T7/13G06T7/136G06T7/149
CPCG06T7/11G06T7/136G06T7/149G06T7/13G06T7/0012G06T2207/20032G06T5/70
Inventor 徐小波
Owner 维柯基科技上海有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com