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Rapid propagation method for lily non-toxic seedlings

A technology of non-toxic seedlings and lily, which is applied in the field of rapid propagation of non-toxic lily seedlings, can solve the problems of poor cultivation effect, degeneration of bulbs, and low reproduction coefficient, and achieve the advantages of convenient detoxification and sterilization, strong vitality, and short breeding period Effect

Pending Publication Date: 2021-10-08
重庆临空都市农业开发建设有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are common problems in non-toxic seedling propagation methods such as poor cultivation effect, low reproduction coefficient, and obvious bulb degradation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for rapid propagation of lily non-toxic seedlings, comprising the following steps:

[0035] 1. Disinfection of explants

[0036] Select the buds of lily lily with leaf primordia as explants, soak them in nutrient solution for 15 minutes at 18-20°C, rinse with water for 1 hour, put them in saturated bleach for 10 minutes, and then wash them with sterile water for 5 times , dry the water with sterile filter paper, peel off part of the leaf primordia, and cut the plant buds obliquely into 0.5-1.5 cm long pieces for later use;

[0037] 2. Induction of callus

[0038] The explants cut into small sections were inoculated in the prepared medium I and cultured for 6 weeks until the incision of the plant buds formed obvious protrusions and callus tissue was formed. The medium I was supplemented with 0.5 mg / L BA and 0.1 MS medium with mg / L NAA; culture temperature 20-24°C, light intensity 2000lx, light time 8-12h per day.

[0039] 3. Induction of Adventitious Buds

...

Embodiment 2

[0050] A method for rapid propagation of lily non-toxic seedlings, comprising the following steps:

[0051] 1. Disinfection of explants

[0052] Select the buds of lily lily with leaf primordia as explants, soak in nutrient solution for 20 minutes at 18-20°C, rinse with water for 1 hour, put them in saturated bleach for 10 minutes, and then wash them with sterile water for 3~ 6 times, dry the water with sterile filter paper, peel off part of the leaf primordia, and cut the buds obliquely into 0.5-1.5cm long pieces for later use;

[0053] 2. Induction of callus

[0054] The explants cut into small sections were inoculated in the prepared medium I and cultured for 8 weeks until the incision of the plant buds formed obvious protrusions and callus tissue was formed. The medium I was supplemented with 0.5 mg / L BA and 0.1 MS medium with mg / L NAA; culture temperature 20-24°C, light intensity 2000lx, light time 8-12h per day.

[0055] 3. Induction of Adventitious Buds

[0056] Cut t...

Embodiment 3

[0066] A method for rapid propagation of lily non-toxic seedlings, comprising the following steps:

[0067] 1. Disinfection of explants

[0068] Select the buds of lily lily with leaf primordia as explants, soak in nutrient solution for 18 minutes at 18-20°C, rinse with water for 1 hour, put them in saturated bleach for 10 minutes, and then wash them with sterile water for 3~ 6 times, dry the water with sterile filter paper, peel off part of the leaf primordia, and cut the buds obliquely into 0.5-1.5cm long pieces for later use;

[0069] 2. Induction of callus

[0070] The explants cut into small sections were inoculated in the prepared medium I and cultured for 7 weeks until the incision of the plant buds formed obvious protrusions and callus tissue was formed. The medium I was supplemented with 0.5 mg / L BA and 0.1 MS medium with mg / L NAA; culture temperature 20-24°C, light intensity 2000lx, light time 8-12h per day.

[0071] 3. Induction of Adventitious Buds

[0072] Cut...

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PUM

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Abstract

The invention provides a rapid propagation method for lily non-toxic seedlings. The rapid propagation method comprises the following steps of selecting plant buds with leaf primordia of lilium lancifolium as explants, and culturing to form seedlings correspondingly through the processes of explant disinfection, callus induction, adventitious bud induction, adventitious bud proliferation, adventitious root induction, hardening-seedling transplantation and the like. According to the rapid propagation method, the propagated seedlings are vigorous, detoxification and sterilization are convenient, and the survival rate of the seedlings is high.

Description

technical field [0001] The invention is applied in the technical field of lily planting, and in particular relates to a method for rapid propagation of non-toxic lily seedlings. Background technique [0002] Lily, also known as Qiangshu, Fanjiu, Shandan, Daoxian, Chongmai, Zhongting, Moro, Zhongfeng, Zhongfenghua, Lily Garlic, Master Garlic, Garlic Brain Potato, Nocturnal Lily, etc., is a lily of the Liliaceae family. It is a perennial herbaceous bulbous plant, native to China, mainly distributed in the temperate regions of the northern hemisphere such as eastern Asia, Europe, and North America. At least 120 species have been found in the world, 55 of which are produced in China. Lily lily is very popular because of its bright colors, large flowers, long flowering period, graceful posture, and its rhizomes can be used as medicine. Lilies usually adopt vegetative propagation methods, such as using bulbs and scales, plant buds, stems, etc. to reproduce, but these methods will...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 唐冰霞欧建龙毛军胡黎华江建明黄振霖赵雨佳王宇彤黄小惠
Owner 重庆临空都市农业开发建设有限公司
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