Non-lethal freshwater fish fin ray strontium marking method and detection method thereof

A technology of marking method and detection method, applied in fish farming, measuring devices, instruments, etc., can solve problems such as fish death, and achieve the effect of avoiding damage

Pending Publication Date: 2021-10-26
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the technical problem that the existing fish marking technology must lead to the death of the fish body during sampling after the marking is completed, the present invention provides a non-lethal freshwater fish fin ray strontium marking and detection method, which can be used in non-lethal, low-damage Convenient, fast, simple, efficient and objective freshwater fish marking and detection technology

Method used

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  • Non-lethal freshwater fish fin ray strontium marking method and detection method thereof
  • Non-lethal freshwater fish fin ray strontium marking method and detection method thereof
  • Non-lethal freshwater fish fin ray strontium marking method and detection method thereof

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Effect test

Embodiment 1

[0036] A non-lethal freshwater fish fin ray strontium marking method adopts the following process steps:

[0037] Step a: configure a strontium chloride hexahydrate marking solution in a pp culture tank, and the concentration of strontium ions is 800mg / L;

[0038] Step b: putting the juvenile bream into the breeding tank and exposing for 5 days;

[0039] Step c: After the exposure was completed, the fish were removed from the exposure container, rinsed three times with aerated tap water without contamination from the marked solution, and then put into a glass tank filled with aerated tap water for breeding.

[0040] The detection method of the fish fin rays marked by the above-mentioned non-lethal freshwater fish fin ray strontium marking method, the steps include:

[0041] Step a: During detection, a section of dorsal fin is removed near the base;

[0042] Step b: After the dorsal fin is cleaned and dried, first paste 10 fin rays in a flat manner on the solidified resin she...

Embodiment 2

[0046] A non-lethal freshwater fish fin ray strontium marking method adopts the following process steps:

[0047] Step a: configure a strontium chloride hexahydrate marking solution in a pp culture tank, and the concentration of strontium ions is 800mg / L;

[0048] Step b: putting the juvenile bream into the breeding tank and exposing for 5 days;

[0049] Step c: After the exposure is completed, the fish is removed from the exposure container, rinsed three times with aerated tap water that is not polluted by the marked solution, and then put into a glass tank filled with aerated tap water for breeding;

[0050] The detection method of the fish fin rays marked by the above-mentioned non-lethal freshwater fish fin ray strontium marking method, the steps include:

[0051] Step a: During detection, a section of pelvic fin is removed near the base;

[0052] Step b: After cleaning and drying the pelvic fins, first paste 15 fin rays in a flat laying manner on the solidified resin sh...

Embodiment 3

[0056] A non-lethal freshwater fish fin ray strontium labeling and detection method adopts the following process steps:

[0057] Step a: configure strontium chloride hexahydrate labeling solution in the pp culture tank, the concentration of strontium ion is 800mg / L;

[0058] Step b: putting juvenile bream into the breeding tank and exposing for 5 days;

[0059] Step c: After the exposure is completed, the fish are removed from the exposure container, rinsed three times with aerated tap water that is not contaminated by the marked solution, and then put into a glass tank filled with aerated tap water for breeding;

[0060] The detection method of the fish fin rays marked by the above-mentioned non-lethal freshwater fish fin ray strontium marking method, the steps include:

[0061] Step a: When testing, pick a section of pectoral fin rays and caudal fin rays near the base;

[0062] Step b: After cleaning and drying the pelvic fins, paste 10 sets of pectoral fin rays and caudal...

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Abstract

The invention discloses a non-lethal freshwater fish fin ray strontium marking method. The method comprises the following steps of a, preparing a strontium marking solution in a marking container, b, transferring the to-be-marked fish into the marking solution in the marking container, and performing short-term exposure, so that the strontium element is deposited on fin rays of the marked fish, and c, after exposure is completed, moving the fish out of the marking container. The invention also discloses a detection method of the non-lethal freshwater fish fin ray strontium marking method, which comprises the following steps of a, capturing a to-be-detected fish, and picking fin rays, b, after the fin ray is cleaned, dried, embedded, sliced and polished, enabling the fin ray to be ultrasonically cleaned and dried, and c, analyzing the strontium content of the fin ray by using an electronic probe micro-area analyzer, determining whether the fin is a marked fish or not according to an analysis result whether a high-strontium mark feature exists or not, if the high-strontium mark feature exists, determining that the fin is the marked fish, and if the high-strontium mark feature does not exist, determining that the fin is not the marked fish.

Description

technical field [0001] The invention belongs to the technical field of evaluation and protection of aquatic wild animal resources, and in particular relates to a non-lethal freshwater fish fin ray strontium marking method and a detection method thereof. Background technique [0002] As an important national policy of our country, the multiplication and release work has achieved good economic, social and ecological benefits, and has played an important role in the recovery of some key water areas or aquatic biological resources of fine varieties. Although the breeding and release work has shown many benefits, there are still limitations such as an imperfect effect evaluation system and a necessary and objective evaluation technology system that has not been completed. The main reason for the above limitations is the lack of a convenient, effective and objective release biomarker technology. Therefore, there is an urgent need for a long-lasting and objectively reflected relea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N23/2202G01N23/2252A01K61/95
CPCG01N23/2202G01N23/2252A01K61/95G01N2223/079G01N2223/102G01N2223/6126
Inventor 姜涛杨健朱亚华刘洪波陈修报
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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