Fig callus culture method

Abstract

The invention discloses a fig callus culture method. The method comprises the following steps of (1) asepsis treatment of stem segments: cleaning stems with axillary buds of a plant material, removing branches, disinfecting, cleaning, cutting the stem segments short, and inoculating a culture medium with morphological lower ends; (2) culturing the stems of aseptic fig seedlings: culturing under a shading condition, inducing calluses from edge tissues of the stems, cutting off the calluses, putting the calluses into a subculture medium, and culturing until the volume is 1-2 times of the original volume and the color is yellowish white for later use; and (3) screening a optimal culture medium and culture condition. According to the culture method of the 'Mareuyuefen' callus, the induction rate can reach 90%, and a new way is provided for molecular biology and genetic engineering research of the 'Mareuyuefen'.

Description

technical field [0001] The invention relates to a fig cultivation method, in particular to a fig callus cultivation method. Background technique [0002] Fig fruit is rich in various amino acids and mineral elements needed by the human body, and has both medicinal, edible and ornamental values. At present, most of the researches on figs focus on nutritional value, fruit ripening, flower bud differentiation and so on. At the level of molecular biology, model plants such as Arabidopsis thaliana and tobacco can be selected for gene function verification, and the verification of native plants is more conducive to clarifying the problem. At present, the number of articles reporting fig transgenics is relatively small. Therefore, in the study of fig molecular biology, it is urgent to explore a new direction to improve the functional verification of fig genes. In the study of plant molecular mechanism, callus is a commonly used experimental material, and it is also a necessary ma...

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Problems solved by technology

The fruit of fig is a cryptocephalic inflorescence, which is named after the appearance of the fruit but not the flowers. The ripening period of the fruit in Jiangsu Province can last fro

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