Ziziphi spinosae semen and bacillus subtilis co-fermentation product, and preparation method and application thereof

A technology of Bacillus subtilis and jujube seed, which is applied in the field of fermentation engineering, can solve the problems of unsatisfactory sleep aid effect, complicated production method, and expensive raw materials, so as to improve sleep disorders, improve and treat effects, and reduce sleep disorders and depression The effect of symptoms

Pending Publication Date: 2021-10-29
湖北真福医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this food raw material is expensive, and preparation method is more complicated, and the sleep-promoting effect of the product obtained simultaneously is not very ideal

Method used

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  • Ziziphi spinosae semen and bacillus subtilis co-fermentation product, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] This embodiment provides a method for preparing a jujube seed co-fermentation product of Bacillus subtilis, comprising the following steps:

[0030] 1) preparing Bacillus subtilis seed liquid culture medium;

[0031] The preparation method of Bacillus subtilis seed liquid medium is as follows: take 10g of peptone, 5g of glucose, 2g of soybean hydrolyzate, 0.5g of potassium dihydrogen phosphate, and 3g of sodium chloride, add water to 1000mL, adjust the pH to 7.0, and sterilize at 110°C 10min, prepared and set aside.

[0032] Soybean enzymatic hydrolyzate is prepared by the following method:

[0033] S1. After the soybeans are washed, dried and crushed, soybean powder is obtained;

[0034] S2. Soybean powder is added to sterile water, 2wt% bromelain (enzyme activity is 1000U / g) and 1wt% papain (enzyme activity is 700U / g) are added for enzymolysis, after 35°C reaction for 3h, 110°C to inactivate the enzyme 5min, freeze-dried to obtain soybean hydrolyzate.

[0035] 2) ...

Embodiment 2

[0040] This embodiment provides a method for preparing a jujube seed co-fermentation product of Bacillus subtilis, comprising the following steps:

[0041] 2) preparing Bacillus subtilis seed liquid culture medium;

[0042] The preparation method of Bacillus subtilis seed liquid culture medium is as follows: Take 20g of peptone, 10g of glucose, 5g of soybean hydrolyzate, 1g of potassium dihydrogen phosphate, 7g of sodium chloride, add water to 1000mL, adjust the pH to 7.2, and sterilize at 130°C for 20min , prepared, spare.

[0043] Soybean enzymatic hydrolyzate is prepared by the following method:

[0044] S1. After the soybeans are washed, dried and crushed, soybean powder is obtained;

[0045]S2. Soybean powder is added to sterile water, 4wt% bromelain (enzyme activity is 1200U / g) and 3wt% papain (enzyme activity is 1000U / g) are added for enzymolysis, after 40°C reaction for 5h, 120°C to inactivate the enzyme 10 minutes, freeze-dried to obtain soybean hydrolyzate.

[00...

Embodiment 3

[0051] This embodiment provides a method for preparing a jujube seed co-fermentation product of Bacillus subtilis, comprising the following steps:

[0052] 3) preparing the Bacillus subtilis seed liquid culture medium;

[0053] The preparation method of the Bacillus subtilis seed liquid culture medium is as follows: take 15g of peptone, 7g of glucose, 3.5g of soybean hydrolyzate, 0.7g of potassium dihydrogen phosphate, and 5g of sodium chloride, add water to 1000mL, adjust the pH to 7.1, and extinguish at 121°C. Bacteria for 15 minutes, prepared and set aside.

[0054] Soybean enzymatic hydrolyzate is prepared by the following method:

[0055] S1. After the soybeans are washed, dried and crushed, soybean powder is obtained;

[0056] S2. Soybean powder is added to sterile water, 3wt% bromelain (enzyme activity is 1000U / g) and 2wt% papain (enzyme activity is 1000U / g) are added for enzymolysis, after 37°C reaction for 4h, 115°C to inactivate the enzyme 7min, freeze-dried to ob...

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Abstract

The invention provides a ziziphi spinosae semen and bacillus subtilis co-fermentation product and a preparation method and application thereof, and belongs to the technical field of fermentation engineering. The preparation method comprises the following steps of: 1) preparing a bacillus subtilis seed solution culture medium; 2) culturing a bacillus subtilis seed solution; 3) putting the ziziphi spinosae semen powder into a shake flask, adding distilled water, uniformly mixing, and sterilizing to obtain a ziziphi spinosae semen culture medium; 4) inoculating the bacillus subtilis seed solution into the ziziphi spinosae semen culture medium according to 2-10% of the mass of the ziziphi spinosae semen culture medium, putting the ziziphi spinosae semen culture medium into a shaking table, and fermenting for 18-20 hours; and 5) carrying out freeze-drying on a fermentation product, and carrying out crushing and sieving to obtain the ziziphi spinosae semen-bacillus subtilis co-fermentation product. The semen ziziphi spinosae-bacillus subtilis co-fermentation product extract disclosed by the invention has the effect of improving sleep. The ziziphi spinosae semen-bacillus subtilis co-fermentation product can be prepared into medicines for treating insomnia and health-care foods or functional foods for improving the sleep.

Description

technical field [0001] The invention relates to the technical field of fermentation engineering, in particular to a co-fermentation product of Bacillus subtilis and its preparation method and application. Background technique [0002] With the acceleration of the socialization process, people face more and more various pressures and accept more and more external stimuli, and the resulting insomnia and depression are increasing year by year. Sleep is actively regulated by the central nervous system and is closely related to the action of central neurotransmitters. Human sleep-related neurotransmitters mainly include γ-aminobutyric acid (γ-aminobutyric acid, GABA), 5-hydroxytryptamine (5-hydroxytryptamine, 5-HT), acetylcholine (acetylcholine, Ach) and so on. The GABA system is widely distributed in the brain and cerebellum, participates in the regulation of the sleep-wake cycle, and is an inhibitory transmitter system. The 5-HT transmitter system is located in specific parts...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/742A61P25/20C12N1/20A23L33/10A23L33/135C12R1/125
CPCA61K35/742A61P25/20C12N1/20A23L33/10A23L33/135A23V2002/00A23V2200/322
Inventor 任佳鑫范宇鹏
Owner 湖北真福医药有限公司
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