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HE staining method for large hepatic pathological section

A staining method, liver technology, applied in the field of HE staining of liver pathological large slices, can solve the problems such as not applicable to large pathological slices, and achieve the effect of increasing alcohol concentration and action time, uniform coloring, and easing hydration gradient

Pending Publication Date: 2021-11-02
BEIJING TSINGHUA CHANGGUNG HOSPITAL
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Problems solved by technology

[0007] In order to solve the problem that the existing conventional HE staining is not suitable for the preparation of large pathological sections, the present invention provides an HE staining method suitable for large pathological sections of the liver

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  • HE staining method for large hepatic pathological section
  • HE staining method for large hepatic pathological section
  • HE staining method for large hepatic pathological section

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Embodiment 1

[0051] This embodiment provides a method for HE staining of pathological tissue for preparing large pathological sections larger than 5 cm.

[0052] 1. Pretreatment of HE staining

[0053] Such as figure 1 As shown, the postoperative liver tissue was soaked in 10% formalin 10 times the size of the specimen for 24 hours, and the specimens were taken in a continuous radial direction with a thickness of 8-10 mm in the cross-section (parallel to the axial abdominal enhanced CT). Plane drawing). Then the specimens were taken for the second time. In order to ensure the flatness of the large specimens, under the guidance of the embedding frame, the specimens were sliced ​​again at the same angle around the human body, and the thickness of each tissue was controlled between 4-5mm. Fully immerse the tissue in 10% neutral formalin 10 times the size of the tissue and fix it again for 24 hours. After completing the dehydrator baking procedure, enter the HE staining stage.

[0054] 2. T...

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Abstract

The invention relates to the technical field of pathological sections, in particular to an HE staining method for a large hepatic pathological section. Specifically, the HE staining method provided by the invention is adopted to prepare the large liver tissue pathological section which is more than 5cm and can be used for diagnosis and scientific research on the basis of large-scale liver excision tissue of hepatic cholangiocarcinoma. The large liver pathological section prepared by adopting the HE staining method provided by the invention can display the microenvironment around liver tumor and the relationship between the liver tumor and the surrounding tissue in a panoramic manner, and is used for comprehensive clinical diagnosis and scientific research.

Description

technical field [0001] The invention relates to the technical field of pathological slices, in particular to a method for HE staining of liver pathological large slices. Background technique [0002] The adoption of pathological large slide histology began in brain histopathology in the 1970s. The reason for using pathological large slides is to visualize the normal tissue of the brain and its structure and changes, and to correlate the macroscopic and microscopic features and abnormalities of the brain with clinical manifestations, including providing information for emerging imaging techniques at the time. [0003] Pathological large slide histology is applied to genitourinary cancer specimens for the same reasons as neuropathologists. For example, the application of large-section histology has important implications for the understanding of the normal anatomy of the prostate, especially for pathologists and radiologists in training, and the correlation with radiographic ...

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Application Information

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IPC IPC(8): G01N1/30G01N1/36G01N1/28G01N21/84
CPCG01N1/30G01N1/36G01N1/28G01N21/84G01N2001/302
Inventor 金烁李纯曾建平姜楠于劭勍
Owner BEIJING TSINGHUA CHANGGUNG HOSPITAL
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