The invention provides a method for manufacturing a
bone tissue slice by a decalcification method. The method comprises the following steps that S1, bone tissues are taken and put into
paraformaldehyde prepared from PBS to be fixed; S2, the bone tissues are taken out; after being flushed, the bone tissues are put into PBS-EDTA decalcification liquid until the bone tissues are primarily softened; S3, the bone tissues are
cut into tissue blocks; the tissue blocks are put into a container containing inorganic acid decalcification liquid; then, the container is put into a water bath pot for decalcification; S4, the decalcified tissue blocks are flushed for removing acid; then, gradient
dehydration is performed; the tissue blocks are transferred into a container containing
chloroform-absolute ethyl
alcohol mixed liquid, and are then transferred into a container containing
chloroform solution; S5, the taken tissue blocks are subjected to
wax soaking treatment and embedding; after the tissueblocks are
cut into
wax flakes, the
wax flakes are put into
distilled water;
drying is performed; flake sealing is performed after the
dyeing. Two-
step method decalcification is provided; the PBS-EDTAdecalcification liquid has
high calcium ion bonding capability; decalcification is performed under the temperature condition of 34 to 38 DEG C; the pre-decalcification time is effectively shortened;two kinds of acid are mixed for decalcification; the complete decalcification can be promoted; the decalcification speed can be also accelerated.