Method for manufacturing bone tissue slice by decalcification method

Abstract

The invention provides a method for manufacturing a bone tissue slice by a decalcification method. The method comprises the following steps that S1, bone tissues are taken and put into paraformaldehyde prepared from PBS to be fixed; S2, the bone tissues are taken out; after being flushed, the bone tissues are put into PBS-EDTA decalcification liquid until the bone tissues are primarily softened; S3, the bone tissues are cut into tissue blocks; the tissue blocks are put into a container containing inorganic acid decalcification liquid; then, the container is put into a water bath pot for decalcification; S4, the decalcified tissue blocks are flushed for removing acid; then, gradient dehydration is performed; the tissue blocks are transferred into a container containing chloroform-absolute ethyl alcohol mixed liquid, and are then transferred into a container containing chloroform solution; S5, the taken tissue blocks are subjected to wax soaking treatment and embedding; after the tissueblocks are cut into wax flakes, the wax flakes are put into distilled water; drying is performed; flake sealing is performed after the dyeing. Two-step method decalcification is provided; the PBS-EDTAdecalcification liquid has high calcium ion bonding capability; decalcification is performed under the temperature condition of 34 to 38 DEG C; the pre-decalcification time is effectively shortened;two kinds of acid are mixed for decalcification; the complete decalcification can be promoted; the decalcification speed can be also accelerated.

Description

technical field [0001] The invention belongs to the field of microtissue slices, and in particular relates to a method for making bone tissue slices by using a decalcification method. Background technique [0002] In recent years, the incidence of skeletal diseases such as osteoporosis has been increasing, which seriously threatens human health, and skeletal diseases are also a persistent problem in animal husbandry, especially the death and elimination caused by bone metabolism diseases have caused huge losses. Economic loss, with the deepening of bone disease research, bone tissue slices are based on the morphological changes of the disease from the perspective of tissue and cells, and the corresponding diagnosis results are made, while bone histology, pathology and other morphological changes The research, as well as the localization and qualitative research of specific genes and protein expression on bone tissue are inseparable from the production of bone tissue slices. ...

AI Technical Summary

Problems solved by technology

Especially in the early decalcification treatment, if the decalcification of bone tissue is excessive, it will seriously affect the staining effect of tissue cells, or the loss of tissue antigens; if the decalcification is not complete, it will directly affect the production of bone slices, and then affect the pathology of diseased tissues. Diagnostics or scientific experiments can work

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