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Enzymatic preparation method for separating ellagic acid from by-products of rubusoside production

A technology for enzymatic preparation of ellagic acid, applied in organic chemistry, fermentation, etc., can solve the problem of rarely separating ellagic acid, and achieve the effects of low production cost, strong operability, and low equipment requirements

Active Publication Date: 2022-07-01
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] In the prior art, the research on sweet tea polyphenols is mainly aimed at the separation of sweet tea total polyphenols (mixture) or ellagitannin, and there are few reports on the separation of ellagic acid from sweet tea raw materials or from sweet tea by-products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Collection and concentration of the effluent from the upper column of sweet tea: Collect 1 ton of the effluent from the upper column of the macroporous adsorption resin column in the production process of sweet tea, and filter it with a nanofiltration membrane (the molecular weight cut-off of the nanofiltration membrane is 500 Da and the filtration pressure is 0.2 Mpa. ), and the nanofiltration membrane retentate was concentrated to a solid concentration of 13.3% with a nanofiltration membrane to obtain 112L of sweet tea polyphenol concentrate;

[0043] (2) Enzymatic hydrolysis: adjust the pH of the sweet tea polyphenol concentrate to 5.5 with acetic acid, add 0.1 kg of tannase (the sweet tea polyphenol concentrate contains about 4.3 kg of ellagitannins, and the weight of the tannase used is tannin). 2.33% of the weight of flower tannin), stir at a speed of 60 r / min, keep at 37 °C, and enzymatically hydrolyze for 8 hours;

[0044] (3) Organic solvent extraction: Ext...

Embodiment 2

[0050] (1) Collection and concentration of the effluent from the upper column of sweet tea: Collect 1 ton of the effluent from the upper column of the macroporous adsorption resin column in the production process of sweet tea, and filter it with a nanofiltration membrane (the molecular weight cut-off of the nanofiltration membrane is 200Da and the filtration pressure is 0.4Mpa. ), and the nanofiltration membrane retentate was concentrated to a solid concentration of 15.2% with a nanofiltration membrane to obtain 101 L of sweet tea polyphenol concentrate;

[0051] (2) Enzymatic hydrolysis: adjust the pH of the sweet tea polyphenol concentrate to 5.8 with acetic acid, add 0.08 kg of tannase (the sweet tea polyphenol concentrate contains about 4.3 kg of ellagitannins, and the weight of the tannase used is tannin). 1.86% of the weight of flower tannin), stir at a speed of 90 r / min, keep at 35 °C, and enzymatically hydrolyze for 10 hours;

[0052] (3) Organic solvent extraction: ex...

Embodiment 3

[0058] (1) Collection and concentration of the effluent from the upper column of sweet tea: Collect 1 ton of the effluent from the upper column of the macroporous adsorption resin column in the production process of sweet tea, and filter it with a nanofiltration membrane (the molecular weight cut-off of the nanofiltration membrane is 300 Da and the filtration pressure is 0.3 Mpa. ), and the nanofiltration membrane retentate was concentrated to a solid concentration of 12.9% with a nanofiltration membrane to obtain 115L of sweet tea polyphenol concentrate;

[0059] (2) Enzymatic hydrolysis: adjust the pH of the sweet tea polyphenol concentrate to 6.0 with acetic acid, add 0.06 kg of tannase (the sweet tea polyphenol concentrate contains about 4.3 kg of ellagitannins, and the weight of the tannase used is tannin). 1.40% of the weight of flower tannin), stir at a speed of 120 r / min, keep at 32 °C, and enzymatically hydrolyze for 12 hours;

[0060] (3) Organic solvent extraction: ...

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Abstract

An enzymatic preparation method for separating ellagic acid from by-products produced by sweet tea glycosides comprises the following steps: collection and concentration of sweet tea upper column effluent, enzymatic hydrolysis, organic solvent extraction, alkaline water back extraction, acidification and crystallization. The content of the fine ellagic acid obtained by the method of the invention is as high as 98.63%; the invention fills the gap of the prior art, and provides a brand-new, comprehensive utilization of sweet tea resources, and separation of ellagic acid from the by-product of sweet tea glycoside production The enzymatic preparation method of acid can turn waste into treasure, the process has strong operability, low equipment requirements, low production cost, safety, environmental protection, and is suitable for industrial production.

Description

technical field [0001] The invention relates to a preparation method for separating ellagic acid from sweet tea, in particular to a preparation method for separating ellagic acid from by-products produced by sweet tea glycosides. Background technique [0002] Sweet tea (scientific name Rubus Suavissimus S.Lee, English name Sweet tea), also known as sweet leaf Rubus, is a perennial thorny shrub of the Rosaceae Rubus genus, which was discovered as a drug in my country in the early 1980s. Sweet tea is sweet and refreshing, not only can promote body fluid and quench thirst, but also have better pharmacological effects and health care functions on the human body. According to the records of "Chinese Medicine Chronicles" and related literature reports: "Sweet tea can prevent and treat hypertension, damp-heat dysentery, skin pruritus, carbuncle and malignant sores, and has the functions of nourishing liver and kidney and stomach, reducing inversion, moistening lung and relieving co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D493/06C12P17/18
CPCC07D493/06C12P17/181
Inventor 李伟黄华学
Owner HUNAN HUACHENG BIOTECH