Base coupling efficiency detection method and device
A technology of coupling efficiency and detection method, applied in the field of communication, can solve the problems of insufficient accuracy and completeness of data analysis results, and insufficient stability of DMT detection methods.
Active Publication Date: 2021-11-16
天津擎科生物技术有限公司 +1
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[0005] The invention provides a base coupling efficiency detection method, device, electronic equipment and storage medium, which are used to solve the defects that the DMT detection method in the prior art is not stable enough, and the data analysis results are not accurate and complete, and realize the high efficiency and stability of DMT detection
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[0045] In order to make the purpose, technical solutions and advantages of the present invention clearer, the technical solutions in the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the present invention. Obviously, the described embodiments are part of the embodiments of the present invention , but not all examples. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.
[0046] DNA chemical synthesis is carried out from the 3' end to the 5' end. The specific synthesis steps are as follows:
[0047] Deprotection group, use trichloroacetic acid to remove the protective group DMT of the nucleotide connected to CPG, and obtain a free 5'-hydroxyl terminal for the next step of condensation reaction.
[0048] Activation, the protonated nucleoside 3'-phosphoramidite...
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The invention provides a base coupling efficiency detection method and device. The method comprises the following steps: determining an actual value of the color depth of dimethoxytriphenylmethyl in a process of synthesizing a base with a preset molar mass; determining a reference value of the color depth of the dimethoxy trityl in the process of synthesizing the basic group; and determining the coupling efficiency of the basic group according to the proportion between the actual value and the reference value. According to the base coupling efficiency detection method and device provided by the embodiment of the invention, the actual value and the reference value of the DMT color depth change in the base synthesis process are determined in real time, the base coupling efficiency is represented through the proportion between the actual value and the reference value, and the complete and accurate analysis is carried out on data.
Description
technical field [0001] The invention relates to the field of communication technology, in particular to a method and device for detecting base coupling efficiency. Background technique [0002] During the synthesis of deoxyribose nucleic acid (Deoxyribonucleic Acid, DNA), the deprotection group (Deblocking) removes the nucleotides connected to the controlled pore glass (Controlled Pore Glass, CPG) with trichloroacetic acid (TCA) The protective group dimethoxytrityl (DMT) to obtain a free 5'-hydroxyl end for the subsequent condensation reaction. [0003] DMT detection mainly detects the color depth after the reaction of dimethoxytrityl, converts the color depth into data for further analysis, and calculates the coupling efficiency of bases. [0004] However, the serial communication used in the DMT detection system in the prior art can only be backward compatible with various synthesizers, and the detection method is not stable enough, and the data analysis results are not a...
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Login to View More IPC IPC(8): G16B20/00G16B15/00G06F17/18G01N21/25
CPCG16B20/00G16B15/00G06F17/18G01N21/25
Inventor 马石金苑永军杜军陈园园
Owner 天津擎科生物技术有限公司