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Glutamine synthetase mutant with glufosinate-ammonium resistance, nucleic acid molecule and application

A technology of glutamine and nucleic acid molecules, which is applied in the field of genetic engineering, can solve the problems of affecting plant growth and development, interfering with plant nitrogen metabolism, and insufficient tolerance of glufosinate-ammonium for commercial application, so as to achieve easy acceptance and satisfy growth and development Effect

Pending Publication Date: 2021-12-03
SICHUAN GEVOTO BIOTECH CO LTD
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  • Description
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AI Technical Summary

Problems solved by technology

[0004] Studies have shown that glufosinate-ammonium acetylase encoded by bar gene and pat gene can inactivate glufosinate-ammonium, but before glufosinate-ammonium contacts GS, it is difficult for glufosinate-ammonium acetylase to completely inactivate glufosinate-ammonium Live, because many GS are distributed on the cell membrane, some non-inactivated glufosinate can inhibit the activity of GS on the cell membrane, thereby interfering with the nitrogen metabolism of plants
Therefore, when glufosinate-ammonium is applied to crops with bar gene and pat gene, it will interfere with the nitrogen metabolism of plants to varying degrees, and at the same time affect the normal growth and development of plants.
Although the sensitivity of transgenic plants to glufosinate can be reduced to a certain extent by overexpressing wild-type GS in plants, its tolerance to glufosinate is far from enough for commercial application

Method used

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  • Glutamine synthetase mutant with glufosinate-ammonium resistance, nucleic acid molecule and application
  • Glutamine synthetase mutant with glufosinate-ammonium resistance, nucleic acid molecule and application
  • Glutamine synthetase mutant with glufosinate-ammonium resistance, nucleic acid molecule and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] The rice (Oryza sativa) glutamine synthetase (GS1) mutant provided in this embodiment is composed of the wild-type rice glutamine synthetase itself (named OWT, the amino acid sequence is shown in SEQ ID NO.1, and the encoding nucleus The nucleotide sequence is that the 57th amino acid residue G of SEQ ID NO.6) is mutated into A, C, D, E, F, H, I, K, L, M, N, P, Q, R, S, V, W, Y or deletion, the obtained rice GS1 mutants were named OG57A, OG57C, OG57D, OG57E, OG57F, OG57H, OG57I, OG57K, OG57L, OG57M, OG57N, OG57P, OG57Q, OG57R, OG57S, OG57V, OG57W, OG57Y, and OG57X.

[0096] Amino acid sequence comparison of rice GS1 mutants OG57A, OG57C, OG57D, OG57E, OG57F, OG57H, OG57I, OG57K, OG57L, OG57M, OG57N, OG57P, OG57Q, OG57R, OG57S, OG57V, OG57W, OG57Y and OG57X and wild-type rice GS1 For example figure 1 As shown in the figure: the position indicated by the arrow is the mutation site.

[0097] In this example, the coding sequence of each rice GS1 mutant is at the positio...

Embodiment 2

[0101] The soybean (Glycine max) GS1 mutant provided in this example is composed of the wild-type soybean GS1 itself (named GWT, the amino acid sequence is shown in SEQ ID NO.3, and the encoding nucleotide sequence is SEQ ID NO.8). The 57th position (corresponding to the 57th position of the reference sequence (SEQ ID NO.1)) is mutated from amino acid residue G to C, D, E, F, H, I, K, L, M, N, P, Q , R, S, T, V, W, Y or deleted. The obtained soybean GS1 mutants were named GG57C, GG57D, GG57E, GG57F, GG57H, GG57I, GG57K, GG57L, GG57M, GG57N, GG57P, GG57Q, GG57R, GG57S, GG57T, GG57V, GG57W, GG57Y and GG57X.

[0102] Amino acid sequences of soybean GS1 mutants GG57C, GG57D, GG57E, GG57F, GG57H, GG57I, GG57K, GG57L, GG57M, GG57N, GG57P, GG57Q, GG57R, GG57S, GG57T, GG57V, GG57W, GG57Y and GG57X compared with wild type GS1G For example figure 2 As shown in the figure: the position indicated by the arrow is the mutation site.

[0103] The coding sequences of soybean GS1 mutants G...

Embodiment 3

[0108] The maize (Zea mays) GS1 mutant provided in this example is composed of the wild-type maize GS1 itself (named ZWT, the amino acid sequence is shown in SEQ ID NO.2, and the encoding nucleotide sequence is SEQ ID NO.7). The 57th position (corresponding to the 57th position of the reference sequence (SEQ ID NO.1)) is mutated from amino acid residue G to C, D, E, F, H, I, K, L, M, N, P, Q , R, S, T, V, W, Y or deleted. The maize GS1 mutants obtained were named ZG57C, ZG57D, ZG57E, ZG57F, ZG57H, ZG57I, ZG57K, ZG57L, ZG57M, ZG57N, ZG57P, ZG57Q, ZG57R, ZG57S, ZG57T, ZG57V, ZG57W, ZG57Y and ZG57X.

[0109] Amino acid sequence comparison of maize GS1 mutants ZG57C, ZG57D, ZG57E, ZG57F, ZG57H, ZG57I, ZG57K, ZG57L, ZG57M, ZG57N, ZG57P, ZG57Q, ZG57R, ZG57S, ZG57T, ZG57V, ZG57W, ZG57Y and ZG57X and wild type maize GS1ZW For example image 3 As shown in the figure: the position indicated by the arrow is the mutation site.

[0110] In this example, the coding sequence of each maize...

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Abstract

The invention discloses a glutamine synthetase mutant with glufosinate-ammonium resistance, a nucleic acid molecule and an application, and relates to the technical field of gene engineering. The inventor discovers that the glutamine synthetase mutant can be obtained by mutation at the nth site of wild glutamine synthetase. After mutation, the nth site is transformed into C, E, F, I, M, N, P, S and Y or deleted, and the mutation can endow the glutamine synthetase with glufosinate-ammonium resistance suitable for commercial application. The glutamine synthetase mutant has application potentials of constructing expression vectors for transforming plants and cultivating glufosinate-ammonium-resistant crops.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a glutamine synthetase mutant with glufosinate-ammonium resistance, nucleic acid molecule and application. Background technique [0002] Glufosinate-ammonium, also known as glufosinate, the trade name is basta, baston, and the chemical name is 4-[hydroxy(methyl)phosphono]-DL-homoalanine or 2-amino- Ammonium 4-[hydroxy(methyl)phosphono]butyrate. Broad-spectrum contact herbicide developed by Bayer. By inhibiting the activity of glutamine synthetase (Glutamine synthetase, GS), the synthesis of glutamine in plants is blocked, and then the nitrogen metabolism in plants is disordered, the synthesis of proteins and nucleotides and other substances is reduced, and photosynthesis is blocked. Chlorophyll synthesis decreased. At the same time, the content of ammonium ions in the cells increases, causing the cell membranes to be damaged, the chloroplasts to disintegrate, and e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/00C12N15/52C12N15/70C12N1/21C12R1/19
CPCC12N9/93C12N15/70C12Y603/01002
Inventor 邓龙群张震陈容侯青江胥南飞
Owner SICHUAN GEVOTO BIOTECH CO LTD
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