Bioreactor exhaust pipeline, bioreactor and exhaust pipeline control method

Abstract

The embodiment of the invention provides a bioreactor exhaust pipeline, a bioreactor and an exhaust pipeline control method. The bioreactor exhaust pipeline comprises a controller, a first pipeline and a second pipeline; each of the first pipeline and the second pipeline comprises a sterilization pipeline and a filtering pipeline; one end of the filtering pipeline is connected with the reactor tank body, and the other end is connected with the exhaust port; one end of the sterilization pipeline is connected with the steam outlet, and the other end is connected with the drain outlet; the controller is used for opening the filtering pipeline of the current standby pipeline, closing the filtering pipeline of the working pipeline and opening the sterilizing pipeline of the working pipeline when the pressure in the reactor tank body is larger than or equal to a preset threshold value, and when the first pipeline serves as the working pipeline, the second pipeline serves as the standby pipeline and when the second pipeline serves as the working pipeline, the first pipeline serves as a standby pipeline. According to the embodiment of the invention, the problem that bacteria in the air reversely pollute the cell culture fluid in the reactor through the exhaust pipeline is solved.

Description

technical field [0001] The embodiments of the present invention relate to the field of bioreactors, and in particular to a bioreactor exhaust pipe, a bioreactor and a method for controlling the exhaust pipe. Background technique [0002] The normal cell culture temperature is generally 27-37°C, and the culture period is generally 3-30 days. Therefore, some cell culture processes require a 37°C, 30-day culture cycle. When the deep layer of the tank is continuously ventilated, the exhaust gas often contains a lot of water vapor. And because the filter needs to be equipped with a hydrophobic filter element, the air cannot pass through the water, so the filter element in the filter is often blocked by the water vapor in the exhaust gas, resulting in the failure of normal exhaust, the problem of rising pressure in the tank, and the failure of cell culture. Contents of the invention [0003] The embodiment of the present invention provides a bioreactor exhaust pipe, bioreactor...

AI Technical Summary

Problems solved by technology

[0003] The embodiment of the present invention provides a bioreactor exhaust pipe, bioreactor and exhaust pipe control method to solve the problem that the filter element in the filter of the existing bioreactor is often blocked by water vapor in the exhaust gas, resulting in failure to exhaust normally. There is a problem that the pressure in the tank increases and the cell culture fails

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