A method for improving the extraction rate of chondroitin sulfate prepared from tilapia skull

A chondroitin sulfate and tilapia technology, applied in the field of bioengineering, can solve the problems of high time requirements, complex steps, long production cycle, etc., and achieve the effects of mild reaction conditions, improved extraction rate, and efficient extraction.

Active Publication Date: 2022-03-11
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the alkali-salt method will pollute the environment and degrade chondroitin sulfate; ultrasonic assistance requires high time and is prone to impurities, and the efficiency of neutral salt extraction of chondroitin sulfate is low
The separation and purification methods of chondroitin sulfate include chromatography, electrophoresis ultrafiltration, anion exchange resin method, etc., but these methods have problems such as complicated steps, long production cycle and high cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] This example provides a test for preparing chondroitin sulfate by applying a method for improving the extraction rate of chondroitin sulfate prepared from tilapia skull.

[0024] (1) Tilapia skulls were steamed at 100°C for 30 minutes to remove excess muscle and other impurities, soaked in ethanol overnight, dried in an oven at 65°C for 8 hours, crushed into powder, and stored at -80°C for later use.

[0025] (2) Take 10g of the fish skull powder prepared in (1), add 50mM Na 2 CO 3 solution, adjust the pH=7.0, then add 200 μL Savinase 16L, stir in a water bath at 55°C for 4 hours, and inactivate the enzyme at 100°C for 10 minutes. Cool to room temperature to obtain the first enzymolysis solution.

[0026] (3) Add 5.4 mg / ml 2709 alkaline protease to the first enzymolysis solution obtained in (2), react in a water bath at 50°C for 2 hours, and inactivate the enzyme at 100°C for 10 minutes. Cool to 4°C, centrifuge at 8000r / min for 20min, collect the supernatant, and obt...

Embodiment 2

[0031] This example provides a test for preparing chondroitin sulfate by applying a method for improving the extraction rate of chondroitin sulfate prepared from tilapia skull.

[0032] The operation process is the same as that in Example 1, except that the mass-volume ratio of tilapia skull powder to Savinase 16L in step (2) is 1:15 in g:μL; The mass-to-volume ratio of the solution is 5:1 in mg:mL.

Embodiment 3

[0034] This example provides a test for preparing chondroitin sulfate by applying a method for improving the extraction rate of chondroitin sulfate prepared from tilapia skull.

[0035]The operation process is the same as that in Example 1, except that the mass-to-volume ratio of tilapia skull powder to Savinase 16L in step (2) is 1:25 in g:μL; The mass-to-volume ratio of the solution is 6:1 in mg:mL.

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PUM

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Abstract

The invention provides a method for preparing chondroitin sulfate from tilapia skull, comprising adopting Savinase 16L to enzymatically hydrolyze tilapia skull powder to obtain Savinase 16L enzymatic solution; Secondary enzymatic hydrolysis; the chondroitin sulfate is obtained by separation and purification with ethanol and cetylpyridinium chloride. The present invention adopts the compound enzymatic hydrolysis method of Savinase 16L and 2709 alkaline protease to effectively realize the efficient extraction of chondroitin sulfate in tilapia skull powder, and further realizes the extraction of chondroitin sulfate by ethanol and cetylpyridinium chloride The purification of tilapia by-products can be used with high value.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a method for improving the extraction rate of chondroitin sulfate prepared from tilapia skulls. Background technique [0002] Chondroitin Sulfate (CS) is a highly sulfated glycosaminoglycan composed of alternating D-glucuronic acid and N-acetyl-D-galactosamine-linked disaccharide units. According to the number and types of sulfuric acid groups attached to different positions of glucuronic acid (GlcA) and N-acetylgalactosamine (GalNAc), there are many isomers of chondroitin sulfate. According to existing studies, chondroitin sulfate has various biological activities such as anti-inflammation, anti-oxidation, anti-coagulation, hypolipidemic and anti-tumor, and effectively inhibits thrombus formation, hinders atherosclerotic plaque formation and reduces Blood lipid index, thereby preventing cardiovascular disease. In addition, chondroitin sulfate can also be used as a food ad...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08
CPCC08B37/0003C08B37/0069
Inventor 钟赛意左格格陈菁陈建平李瑞宋兵兵陈康健刘晓菲贾学静
Owner GUANGDONG OCEAN UNIVERSITY
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