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Fluorescence detection method of acetylcholin esterase based on carbon dots

A technology of acetylcholinesterase and carbon dots, applied in fluorescence/phosphorescence, chemical instruments and methods, measuring devices, etc., can solve the problems of low sensitivity, complicated operation methods, long reaction time, etc., and achieve high sensitivity, fast response, The effect of low detection limit

Pending Publication Date: 2021-12-10
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most methods have defects such as complicated operation methods, long reaction time, and low sensitivity.

Method used

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  • Fluorescence detection method of acetylcholin esterase based on carbon dots
  • Fluorescence detection method of acetylcholin esterase based on carbon dots
  • Fluorescence detection method of acetylcholin esterase based on carbon dots

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for detecting the concentration of acetylcholinesterase, specifically comprising the steps of:

[0031] 1. Preparation of near-infrared emitting carbon dot nanoparticles CDs:

[0032] Weigh 0.6g of glutathione and 19.4g of formamide solution, mix them ultrasonically for 30min, put them into a 50mL polytetrafluoroethylene autoclave, and react at 160°C for 10h. Porous membrane filtration, after filtration, dialyze with a dialysis bag with a molecular weight of 1000 for two days, and finally freeze the product and put it in a freeze dryer for two days to freeze-dry to obtain near-infrared emitting carbon dots.

[0033] 2. Preparation of oxidized near-infrared emitting carbon dot nanoparticles CDs-HNO 3 :

[0034] Weigh 10 mg of the near-infrared emitting carbon dot nanoparticles CDs prepared in step 1, and measure 17 μL of HNO with a concentration of 14.5 mol / L 3 , the HNO 3 Dilute with 20mL deionized water, and then dissolve the weighed near-infrared emitting...

Embodiment 2

[0044] A method for detecting the concentration of acetylcholinesterase, specifically comprising the steps of:

[0045] 1. Preparation of near-infrared emitting carbon dot nanoparticles CDs: the same as in Example 1;

[0046] 2. Preparation of oxidized near-infrared emitting carbon dot nanoparticles CDs-HNO 3 :

[0047] Weigh 10 mg of the near-infrared emitting carbon dot nanoparticles CDs prepared in step 1, and measure 17 μL of HNO with a concentration of 14.5 mol / L 3 , the HNO 3 Dilute with 20mL deionized water, and then dissolve the weighed near-infrared emitting carbon dot nanoparticles CDs into the diluted HNO 3 solution, after mixing evenly, add the mixed solution into a 50mL round-bottomed flask, and reflux with magnetic stirring in an oil bath at 60°C for 24 hours. properties, and then filtered with a 0.22-micron microporous membrane, and then dialyzed with a dialysis bag with a molecular weight of 1000 for 24 hours. Finally, the product was frozen and put into a ...

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Abstract

The invention provides a method for detecting the concentration of acetylcholin esterase. Nitric acid is used for treating carbon dots capable of emitting near-infrared light to obtain oxidized carbon dots CDs-HNO3, the surfaces of the CDs-HNO3 contain a large number of water-soluble groups, and strong coordination and electrostatic interaction can be generated between Ag<+> and the CDs-HNO3 to cause aggregation of the carbon dots, so that the fluorescence emission peak of the CDs-HNO3 can be obviously quenched. AChE can catalytically hydrolyze acetylthiocholine (ATCh) and generate thiocholine (TCh), and the binding capacity of sulfydryl on the TCh and Ag<+> is higher. Therefore, AgNO3 can be used for processing the CDs-HNO3 to obtain Ag<+>-CDs-HNO3 with extremely weak fluorescence intensity, so that the fluorescence intensity of the Ag<+>-CDs-HNO3 is enhanced, and the concentration of AChE in the solution can be quantitatively detected by measuring the fluorescence enhancement intensity of the Ag<+>-CDs-HNO3. The method is simple and convenient in detection process, high in sensitivity, rapid in response and low in detection limit.

Description

technical field [0001] The invention relates to a method for detecting the concentration of acetylcholinesterase, which belongs to the field of detection of acetylcholinesterase. Background technique [0002] Acetylcholinesterase (AChE) is a key enzyme ubiquitously present in the peripheral nervous system, which can specifically catalyze the hydrolysis reaction of the substrate acetylthiocholine to produce thiocholine and acetate. There is a close relationship between Haimer's disease and inflammatory processes and other related diseases, so acetylcholinesterase plays a very important role in biological signal transduction. The high sensitivity, rapid response and easy-to-operate detection of acetylcholinesterase is useful for clinical diagnosis, Alzheimer's The analysis and detection of Haimer's disease drugs and organophosphorus pesticides is very important. At present, the common detection methods of acetylcholinesterase at home and abroad mainly include electrochemical ...

Claims

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Application Information

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IPC IPC(8): G01N21/64C09K11/65C09K11/02
CPCG01N21/6428C09K11/65C09K11/025G01N2021/6432Y02A50/30
Inventor 许子强李海敏孟菘杨清圆
Owner HUBEI UNIV
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