Method for rapidly quantifying synthetic cathinone drugs in urine

A cathinone and drug technology, which is applied in the field of rapid quantification of cathinone-like drugs, can solve the problems of difficult quantitative detection of magnetic bead types and no reported quantitative methods, achieve good sensitivity, overcome poor reproducibility, and overcome sensitivity low effect

Active Publication Date: 2021-12-17
SHANGHAI CRIMINAL SCI TECH RES INST
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Problems solved by technology

[0005] (4-chlorophenyl)-2-(N-pyrrolidinyl)-1-pentanone (abbreviated as 4-Cl-α-PVP), 2-1-(4-methylphenyl)-2-methyl Amino-1-pentanone (abbreviated as 4-MPD) and 3-1-[2-(5,6,7,8-tetrahydronaphthyl)]-2-(N-pyrrolidinyl)-1-pentanone Ketone (abbreviated as β-TH-Naphyrone) covers the substitution of common cathinone derivatives, but there is no report on the quantitative method of β-TH-Naphyrone and 4-MPD, 4-Cl-α-PVP quantitative There are only a few reports
[0010] Magnetic dispersion solid-phase extraction has been widely used in the field of metals and biologically active substances, but there are only few reports on the application in the field of small drug molecules. Lu Q et al. (Graphene oxide-Fe 3 o 4 nanocomposite magnetic solid phase extraction followed by UHPLC-MS / MS for highly sensitive determination of eight psychoactive drugs in urine samples.Talanta.2020Jan 1) Using graphene oxide-Fe 3 o 4 Nanocomposite magnetic adsorbent extracts morphine and amphetamine in urine; Yang F et al. (Magnetic dispersive solid-phase extraction based on modified magnetic nanoparticles for the detection of cocaine and cocaine metabolites in human urine by high-performance liquid chromatography-mass spectroscopy. .2017Nov;40(21):4234-424) with modified Fe 3 o 4 Synthetic nanoparticles extract cocaine and its metabolites from urine, however, the types of magnetic beads in these two methods are difficult to apply to the quantitative detection of synthetic cathinones in urine

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  • Method for rapidly quantifying synthetic cathinone drugs in urine
  • Method for rapidly quantifying synthetic cathinone drugs in urine
  • Method for rapidly quantifying synthetic cathinone drugs in urine

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Experimental program
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Embodiment 1

[0053] Example 1 A method for rapid quantification of 4-Cl-α-PVP, 4-MPD, and β-TH-Naphyrone in urine using magnetic dispersion solid-phase extraction combined with DART-HRMS

[0054] 1) Prepare working solution

[0055] Accurately weigh 4-Cl-α-PVP, 4-MPD, β-TH-Naphyrone, and SKF525A respectively, place them in brown reagent bottles and prepare stock solutions with a concentration of 1.00 mg / ml in methanol, and store them at 4°C for later use. The standard substances methcathinone-D3 (with acetonitrile) and SKF525A (with methanol) were each diluted to 10 μg / ml stock solutions, placed in brown reagent bottles and stored at 4°C for future use.

[0056] Take 1.00mg / ml of the above stock solution to prepare 4-MPD, β-TH-Naphyrone 0.04μg / ml, 0.1μg / ml, 1μg / ml, 4μg / ml, 10μg / ml, 15μg / ml, 20μg / ml ml and mixed standard solutions containing 0.1μg / ml, 0.2μg / ml, 1μg / ml, 4μg / ml, 10μg / ml, 15μg / ml, and 20μg / ml of 4-Cl-α-PVP, store at 4°C Reserved, and then diluted step by step with blank urin...

Embodiment 2

[0106] Embodiment 2 The method of the present invention is used for the quantification of synthetic cathinones in rat urine

[0107] Sprague Dawley male rats (Sprague Dawley) were selected; body weight: 270-310 g; three rats were assigned to each drug. MDPV (Horsley RR et al., Behavioral, Pharmacokinetic, Metabolic, and Hyperthermic Profile of 3,4-Methylenedioxypyrovalerone (MDPV) in the Wistar Rat. Front Psychiatry. 2018 Apr24; 9:144. doi: 10.3389 / fpsyt. 2018.00144.PMID:29740356; PMCID:PMC5928397) and the LD of three synthetic cathinones in this experiment 50 , 4-Cl-α-PVP, 4-MPD and β-TH-Naphyrone were injected subcutaneously at doses of 8.07mg / kg, 5.30mg / kg, and 1.78mg / kg, respectively.

[0108] Rat urine was collected at 4h, 7h, and 24h to detect the original body, and collected once a day after 24h until the original body could not be detected. The standard feed, water, and animal experiments required for all animal experimental feeding were based on the "Experimental An...

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Abstract

The invention relates to a method for rapidly quantifying synthetic cathinone drugs in urine. The method comprises the following steps: by taking methcathinone-D3 and SKF525A as internal standard substances, extracting synthetic cathinone drugs in urine by using hydrophobic magnetic beads through a magnetic dispersive solid-phase extraction (MDSPE), rapidly quantifying by combining a real-time direct analysis high-resolution mass spectrometry (DART-HRMS), and obtaining a quantifying result within one minute after sample introduction, wherein a to-be-detected substance is good in stability under various conditions, the correlation coefficient is larger than 0.99, the deviation of precision and accuracy is smaller than 15%, rapid quantification of the synthesized cathinone drugs in urine is achieved, detection is rapid and convenient, and a new and effective technical support can be provided for rapidly and quantitatively synthesizing cathinone drugs in forensic poison analysis.

Description

technical field [0001] The invention belongs to the field of forensic poison analysis and identification, and in particular relates to a rapid quantification of synthetic cathinones in urine by using magnetic dispersion solid-phase extraction (MDSPE) combined with real-time direct analysis high-resolution mass spectrometry (DART-HRMS) Methods. Background technique [0002] New psychoactive substances (NPS) are drug derivatives designed and illegally manufactured on the basis of the structure of controlled drugs, which are used to imitate the psychoactive effects of controlled drugs and escape criminal sanctions. As of 2019, more than 800 new psychoactive substances have been reported, involving more than 110 countries around the world and still increasing, posing a great challenge to global public safety and described as a "global epidemic". . [0003] Synthetic cathinones, along with synthetic cannabinoids, are the most seized category of NPS worldwide, according to a rec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62G01N1/34G01N1/40
CPCG01N27/62G01N1/34G01N1/405Y02A50/30
Inventor 张玉荣李雅文连茹杨飞宇汪蓉梁晨
Owner SHANGHAI CRIMINAL SCI TECH RES INST
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