Streptococcus strain and vaccine for preventing and treating swine streptococcosis

A technology of Streptococcus suis and strain, applied in the field of veterinary medicine, can solve the problems of non-preventive effect and great stress on pigs

Active Publication Date: 2021-12-24
河南兴华生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commercially available commercial vaccines only have bivalent vaccines against Streptococcus suis type 2 and Type 7, but they cannot prevent Streptococcus s

Method used

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  • Streptococcus strain and vaccine for preventing and treating swine streptococcosis
  • Streptococcus strain and vaccine for preventing and treating swine streptococcosis
  • Streptococcus strain and vaccine for preventing and treating swine streptococcosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1: the isolation of bacterial strain

[0055] 1. Disease material

[0056] Collect brains, lungs, trachea, heart blood, joint fluid, etc. from diseased pigs and dead pigs with dyspnea, sepsis, arthritis and neurological symptoms in pig farms.

[0057] 2. Isolation and culture of bacteria

[0058] Aseptically operate, evenly spread the suspected suis streptococcus disease material on the TSA solid medium containing 10% fetal bovine serum. After culturing for 24 hours in a 37°C incubator. Pick a suspicious single streptococcal colony for purification.

[0059] 3. Serotyping

[0060] The serotypes were identified according to the primer sequences and PCR program in the following table.

[0061] Table 1

[0062]

[0063] 4. Strain preservation

[0064] The correctly identified Streptococcus suis type 2 was named as S4807 strain; Streptococcus suis type 7 was named S8980 strain; The correctly identified Streptococcus suis was added to skimmed milk powder ...

Embodiment 2

[0065] Embodiment 2: Streptococcus suis type 2, type 7 and type 9 antigen preparation

[0066] 1. Source of the strain

[0067] The selected Streptococcus suis type 2 is S4807 strain, and the preservation number is CGMCC No.22747.

[0068] The selected Streptococcus suis type 7 is S8980 strain, and the preservation number is CGMCC No.22748.

[0069] The selected Streptococcus suis type 9 is S1802 strain, and the preservation number is CGMCC No.22749.

[0070] 2. Preparation and inspection of vaccine semi-finished products

[0071] 2.1 Preparation of seed batches for production

[0072] 2.1.1 Propagation of primary seeds

[0073] Dilute S4807 strains, S8980 strains and S1802 freeze-dried bacteria with TSA liquid medium, respectively streak inoculate them with TSB solid medium (containing 10% fetal bovine serum), culture at 37°C for 24 hours, and select off-white transparent small colonies Inoculate in TSB solid medium (containing 10% fetal bovine serum), and cultivate at 3...

Embodiment 4

[0104] Example 4 Safety Inspection

[0105] 1 test plan

[0106] 1.1 Materials: Vaccine A, Vaccine B, Vaccine C, Vaccine D

[0107] 1.2 Method: 25 piglets aged 3 to 4 weeks were selected and randomly divided into 5 groups with 5 piglets in each group. Groups 1 to 4 were intramuscularly injected with overdose of vaccines A to D in the neck, 4ml / head, and group 5 was the control group without immunization, and they were continuously observed for 14 days.

[0108] 2 test results

[0109] Table 7

[0110]

[0111] The results of overdose injection of piglets aged 3 to 4 weeks are shown in Table 7. The results showed that the feed intake, energy, and body temperature of the vaccines A to D were normal, and there were no adverse reactions at the injection site, and no other visible abnormal clinical manifestations; , each organ had no abnormal lesions. It shows that the vaccine is safe and has no side effects.

[0112] Embodiment 4 effectiveness test

[0113] 1. Experiment...

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Abstract

The invention relates to the technical field of animal medicine, in particular to a streptococcus strain and a vaccine for preventing and treating swine streptococcosis. The invention provides streptococcus suis type 2, streptococcus suis type 7 and streptococcus suis type 9 obtained by screening, and the three strains can generate relatively high protection capability on streptococcus suis no matter whether the three strains are used for preparing the vaccine independently or in a mixed mode. Especially, trivalent vaccines prepared from the bacillus subtilis and the bacillus subtilis do not interfere with each other, and can safely and effectively play a good protection role in attack of three pathogenic bacteria.

Description

technical field [0001] The invention relates to the technical field of animal medicine, in particular to a streptococcus strain and a vaccine for preventing and treating streptococcosis suis. Background technique [0002] Swine Streptococxosis is an acute, febrile infectious disease of pigs caused by Streptococcus suis (SS). Under natural conditions, pigs of different ages, breeds and sexes are susceptible to the disease, and the common ones are acute hemorrhagic sepsis, suppurative lymphadenitis, meningitis and arthritis. Among them, sepsis and meningitis are the most harmful, and under the action of some specific inducements, the mortality rate of the diseased pig herds will be reduced. [0003] According to the different cell wall capsular polysaccharides (CPS), Streptococcus suis can be divided into 35 serotypes. However, not all serotypes are pathogenic. Most of the pathogenic serotypes are 1 to 9, among which serotypes 2, 7 and 9 are more common and more virulent. ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K39/09A61K39/39A61P31/04C12R1/46
CPCA61K39/092A61K39/39A61P31/04A61K2039/521A61K2039/55511Y02A50/30
Inventor 牛旻李国强杨青原任素静刘荣飞段雯方高雪李海超刘美焦义领杜婷婷
Owner 河南兴华生物技术有限公司
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