The invention discloses a primer probe combination for identifying a group A
streptococcus M1UK strain and application of the primer probe combination, and belongs to the technical field of
streptococcus mutant strain detection. The primer probe combination provided by the invention can specifically amplify the
target gene of the M1UK strain. Furthermore, a method for identifying the M1UK strain is designed according to the primer probe combination, the M1UK strain and other group A
streptococcus can be effectively distinguished, and besides the advantages of being high in specificity and accurate in detection result, the method is easy, convenient and rapid to operate and high in detection efficiency, labor cost and
time cost are reduced, and the method is suitable for popularization and application. The method can realize rapid and effective non-sequencing-dependent
variant strain detection, and has important significance in
disease prevention and control and improvement of
disease treatment efficiency.