Streptococcus thermophilus and application thereof

A technology of Streptococcus thermophilus and microbial strain, applied in the field of microorganisms

Inactive Publication Date: 2020-10-27
深圳迪健生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, in the prior art, there are relatively few reports on the types of probiotics for preventing or treating oral diseases. Therefore, it is very meaningful to develop a new type of probiotics with the function of preventing or treating oral diseases.

Method used

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  • Streptococcus thermophilus and application thereof
  • Streptococcus thermophilus and application thereof
  • Streptococcus thermophilus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The present embodiment provides a Streptococcus thermophilus (Streptococcus thermophilus) URTOOI bacterial strain for the prevention and treatment of oral diseases involved in the present invention, and the isolation method comprises the following steps:

[0056] (1) Sampling the oral mucosa of healthy children, fasting for half an hour before sampling, and gargling with water three times before sampling;

[0057] (2) Use an oral swab to take a sample, insert the swab head into the left oral cavity, make the swab head fully touch the mucous membrane inside the left cheek, wipe up and down, and rotate the swab at the same time, repeat this action to wipe up and down 30 times, put the swab back into the collection tube to complete the sampling;

[0058] (3) Take out the oral swab, wash it with a small amount of normal saline, inoculate the eluate on the MRS agar plate by streaking, and culture it anaerobically at 37°C for 48 hours, and isolate a single colony;

[0059] (...

Embodiment 2

[0061] The Streptococcus thermophilus that embodiment 1 isolates carries out the observation of bacterium colony form and thalline form, the result is as follows Figure 1-Figure 2 shown. figure 1 It is the colony morphology diagram of Streptococcus thermophilus URTOOI involved in the present invention on the MRS medium, which shows that it grows on the MRS agar plate, and the colony is beige and smooth in shape. figure 2 It is the thalline morphology figure (magnified 1000 times) of the Streptococcus thermophilus URTOOI involved in the present invention under the oil immersion microscope, it shows in the figure: the thalline Gram staining is positive, and the cells are spherical to oval Shaped, with a diameter of 0.8-1.0 μm, two pairs of cocci form chains of different lengths.

Embodiment 3

[0063] The Streptococcus thermophilus isolated in Example 1 was identified by 16S rDNA molecular biology. The genomic DNA of URTOOI single colony culture was extracted as a template, and the bacterial 16S universal primer 27F / 1492R was used for PCR amplification and sequenced. The 16S rDNA base sequence of the streptococcus thermophilus (Streptococcus thermophilus) URTOOI strain involved in the prevention and treatment of oral diseases involved in the present invention is as follows:

[0064] AACCTTATTACTCTACATGCGTGCTGGCTCCAAAGGTTACCTCACCGACTTCGGGTGTTACAAACTCTCGTGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCGACTTCATGTAGGCGAGTTGCAGCCTACAATCCGAACTGAGATTGGCTTTAAGAGATTAGCTTGCCGTCACCGACTCGCAACTCGTTGTACCAACCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTATTACCGGCAGTCTCGCTAGAGTGCCCAACTGAATGATGGCAACTAACAATAGGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAACCATGCACCACCTGTCACCGATGTACCGAAGTAACTTTCTATCTCTAGAAATAGCATCGGGATGTCAAGA...

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Abstract

The invention relates to streptococcus thermophilus and application thereof. The streptococcus thermophilus is named as Streptococcus thermophilus URTOOI strain, wherein the preservation unit is ChinaGeneral Microbiological Culture Collection Center; the preservation time is April 13, 2020, the preservation number is CGMCC No.19575, and the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The streptococcus thermophilus strain has remarkable prevention and treatment effects on oral diseases, can remarkably inhibit adhesion of streptococcus gordonii or streptococcus mutans on hydroxyapatite, can remarkably reduce the thickness and density of a biological membrane formed by the streptococcus mutans on the hydroxyapatite, and can regulate oral flora. The strain is of great significance to prevention and control of oral health and oral diseases.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, relates to a streptococcus thermophilus and application thereof, in particular to a streptococcus thermophilus for preventing and / or treating oral diseases and an application thereof. Background technique [0002] With the improvement of the quality of life of Chinese residents, oral health has become a public health issue of great concern. For a long time, the incidence of some common oral diseases, such as dental caries, periodontal disease and mucosal disease, has been increasing year by year. Oral diseases not only affect people's oral health, but also cause some systemic diseases, and oral pathogenic bacteria are the key factors causing these diseases. Other systemic diseases are of crucial importance. [0003] Probiotics are active microorganisms that are beneficial to the host. They mainly exist in the gastrointestinal tract, oral cavity and reproductive system of the host, and ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/744A61P1/02C12R1/46
CPCA23L33/135A61K35/744A61P1/02A23V2002/00C12R2001/46C12N1/205A23V2400/249A23V2200/312A23V2200/32
Inventor 张兴
Owner 深圳迪健生物科技有限公司
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