66 results about "Streptococcus hemolyticus" patented technology

The present invention relates to nanosilver-containing antibacterial and antifungal granules ("NAGs"). The NAGs have longlasting inhibitory effect on a broad-spectrum of bacteria and fungi, which include, but are not limited to, Escherichia coli, Methicillin resistant Staphylococcus aureus, Chlamydia trachomatis, Providencia stuartii, Vibrio vulnificus, Pneumobacillus, Nitrate-negative bacillus, Staphylococcus aureus, Candida albicans, Bacillus cloacae, Bacillus allantoides, Morgan's bacillus (Salmonella morgani), Pseudomonas maltophila, Pseudomonas aeruginosa, Neisseria gonorrhoeae, Bacillus subtilis, Bacillus foecalis alkaligenes, Streptococcus hemolyticus B, Citrobacter, and Salmonella paratyphi C. The NAGs contain ground stalk marrow of the plant Juncus effusus L. which has been dispersed with nanosilver particles. The nanosilver particles are about 1-100 nm in diameter. Each of the nanosilver particles contain a metallic silver core which is surrounded by silver oxide. The present invention also provides a process for making the NAGs. The NAGs can be used in a variety of healthcare and industrial products. Examples of the healthcare products include, but are not limited to, ointments or lotions to treat skin trauma, soaking solutions or cleansing solutions for dental or women hygiene, medications for treating gastrointestinal bacteria infections, sexual related diseases, and eye diseases. Examples of industrial products include, but are not limited to, food preservatives, water disinfectants, paper disinfectants, construction filling materials (to prevent mold formation).

The present invention relates to nanosilver-containing antibacterial and antifungal granules ("NAGs"). The NAGs have longlasting inhibitory effect on a broad-spectrum of bacteria and fungi, which include, but are not limited to, Escherichia coli, Methicillin resistant Staphylococcus aureus, Chlamydia trachomatis, Providencia stuartii, Vibrio vulnificus, Pneumobacillus, Nitrate-negative bacillus, Staphylococcus aureus, Candida albicans, Bacillus cloacae, Bacillus allantoides, Morgan's bacillus (Salmonella morgani), Pseudomonas maltophila, Pseudomonas aeruginosa, Neisseria gonorrhoeae, Bacillus subtilis, Bacillus foecalis alkaligenes, Streptococcus hemolyticus B, Citrobacter, and Salmonella paratyphi C. The NAGs contain ground stalk marrow of the plant Juncus effuses L. which has been dispersed with nanosilver particles. The nanosilver particles are about 1-100 mn in diameter. Each of the nanosilver particles contain a metallic silver core which is surrounded by silver oxide. The present invention also provides a process for making the NAGs. The NAGs can be used in a variety of healthcare and industrial products. Examples of the healthcare products include, but are not limited to, ointments or lotions to treat skin trauma, soaking solutions or cleansing solutions for dental or women hygiene, medications for treating gastrointestinal bacteria infections, sexual related diseases, and eye diseases. Examples of industrial products include, but are not limited to, food preservatives, water disinfectants, paper disinfectants, construction filling materials (to prevent mold formation).

The present invention provides a gene chip for quickly detecting pathogens from food source, discloses the preparation method of said gene chip and provides 26 oligonucleotide probe sequences for detection. Said gene chip can quickly, accurately and high-effectively detect and identify the class of the pathogens in food, and its detection range includes staphylococcus aureus, Shiga's bacillus, salmonella, colibacillus 0157, bacillus proteus, mononuclear hyperplastic listerella, enterocolitis yersinia, aeruginous pseudomonads, vibrio parahaemolyticus, vibrio cholerae, bacillus cereus, beta hemolytic streptococcus, coconut fermentation pseudomonads, boticin, vibrio jejuni and bacillus perfringens, etc.

The invention belongs to the medical technical field and discloses the application of high-purity in the preparation of antibacterial medicine, antiviral medicine and other medicines. The invention proves the antipyretic, anti-inflammation, immunity enhancement, antibacterial and anti-virus effects of high-purity forsythiaside through the following experiments: (1) the experiment that high-purity forsythiaside has antipyretic effect on fever rats; (2) the experiment that high-purity forsythiaside inhibits xylene-induced mice ear edema, xylene-reduced capillary permeability of mice; (3) the experiment that high-purity forsythiaside increases carbon clearance ability of mice and increases the lymphocyte transformation rate; (4) the experiment that high-purity forsythiaside has vitro anti-influenza A type virus effect and in-vivo anti-respiratory syncytial virus effect; (5) the experiment that high-purity forsythiaside has vitro inhibition effects on beta-hemolytic streptococcus, staphylococcus aureus, streptococcus viridans and staphylococcus epidermidis.

The invention relates to laundry detergent, in particular to efficient laundry detergent with bacterial inhibition and sterilization functions. The laundry detergent comprises components in percentage by weight as follows: 17%-23% of a surfactant, 1%-3% of an acid aid, 0.2%-0.5% of caustic soda flakes, 0.3%-0.5% of a chelating agent, 3%-6% of heartleaf houttuynia herb extract, 0.2%-0.4% of a preservative, 1%-2% of a thickening agent, 0.5%-1% of acorus gramineus essential oil, 1%-2% of fructus amomi essential oil, 1%-2% of licorice extract, 0.6%-1.5% of tea saponin, 0.4%-0.8% of lavender essential oil, 0.6%-1.2% of mint essential oil and the balance of deionized water. The efficient laundry detergent has an inhibiting function on streptococcus hemolyticus, staphylococcus aureus, hemophilus influenza, catarrhalis, pneumococcus, escherichia coli, shigella dysenteriae and salmonella typhi, also has an inhibiting function on various viruses and further has a body immunity enhancing function and an anti-tumor effect.

The mannosan peptide is a kind of fermentation product from Streptococcus hemolyticus-alpha. The mannosan peptide has weight average molecular weight of 10-200 KD and molecular weight distributing coefficient below 4.0. The present invention also provides the preparation process and use of the mannosan peptide.

The invention provides a truncated-form streptococcus hemolyticus bacteriolysin O gene and an ASO detection kit. According to the invention, part of SLO gene fragments are cloned, and the part of SLO genes are cloned into an expression vector of escherichia coli and the like so as to realize mass expression and facilitate purification. In particular, the invention provides a DNA sequence which has a base sequence as shown in SEQ ID NO:1. According to the invention, the truncated-form SLO sequence can greatly improve the yield of SLO protein; more than 150 mg of SLO protein can be obtained through purification of one liter of bacterial culture, and the purity is more than 90%; immunological tests prove that the sectionally expressed SLO still maintains the specific immunogenicity.

The invention discloses a composition for treating diarrhea and a preparation thereof, relating to a medicinal composition for stopping the diarrhea. The invention also relates to a preparation of the medicinal composition for stopping the diarrhea. The medicinal composition is mainly prepared from the main raw materials of 1 part of Racecadotril and 0.25-25 parts of berberine hydrochloride as well as 0.01-250 parts of auxiliary materials in proportion. The medicinal composition has scientific and reasonable prescription, and can be used for inhibiting the rise of calcium ion concentration in colon smooth muscle cells and improving the motion functional coordination of the intestinal tract to realize the effect on stopping the diarrhea mainly by inhibiting the activity of enkephalinase and further inhibiting the excessive secretion of water and electrolyte via the Racecadotril, and simultaneously by the stronger bactericidal effect of the berberine hydrochloride on escherichia coli, Streptococcus hemolyticus, Diplococcus pneumoniae and the like.

The invention relates to a recombinant protein for providing a soluble hemolysis streptolysin O gene and the gene expression, and a method for making the same. The method comprises the steps of: cloning from the streptococcus hemolyticus gene group by using the gene engineering technology to obtain a truncated SLO gene; connecting to an expression vector and transferring into host cell expression to purify a large amount of stable r-s SLO proteins, thereby overcoming various shortcomings of the protein from natural extraction and being favorable for standardization of making various reagents by using the protein as the raw material. The whole-length SLO protein is the protein of about 60 KD containing 571 AA, and the end N is provided with a signal peptide which is used for guiding the mature protein out of the cell. A research shows that the end N containing 77 AA provided with the signal peptide has a hazard function to the host cell, wherein the hazard function is more than a lyticase function which is generated by the latter AA of between 78 and 571.

The invention discloses an essential oil composition prepared by performing rectification and compounding processing on the raw materials comprising 4-terpilenol type tea-tree-oil and eucalyptus grandis x e.urophylla essential oil, and provides the formula, a preparation method and the composition content of the essential oil composition; and the composition has antibacterial insecticidal functions. A gas chromatography method is employed to determine the main content. The method is accurate, stable and easy to operate, is adaptable for industrial production demands, and can help to effectively control the quality of the essential oil composition. The essential oil composition has small toxicity, has different levels of inhibition effects on six pathogens comprising staphylococcus aureus, staphylococcus epidermidis, beta hemolytic streptococcus, escherichia coli, pseudomonas aeruginosa and candida albicans which can cause skin infection, and has antibacterial and insecticidal activities. The preparation method is reasonable and stable, short in production period, suitable for industrial production, and good in application prospect.

Disclosed herein is an immunoassay method for detecting the detection target antigen in an analyte through extraction with an extraction agent. The method is characterized in that the detection is performed in the presence of a cyclic oligosaccharide. The method is also characterized in the use of an immunochromatography kit for detecting gram-positive bacteria in an analyte, and that is configured from an analyte dilution solution, a sample dropping part, an antigen extracting part, a labeling substance retaining part, an immunochromatography medium having a detection part, and an absorption part, and in which an organic acid is retained in the antigen extracting part. The immunochrornatography kit contains a cyclic oligosaccharide and a nitrite compound by containing at least one of a cyclic oligosaccharide, a nitrite compound, and a mixture thereof in the analyte dilution solution and / or the sample dropping part.

The invention discloses antibacterial maca spray. The antibacterial maca spray comprises, by weight, 0.5-1.0% of maca extract liquid, 1.0-1.5% of cinnamon extracts, 0.1-0.15% of clove extract liquid, 0.1-0.15% of capsicum annuum extract liquid, 0.1-0.5% of cistanche deserticola extracts, 0.3-0.5% of chlorhexidine acetate and the balance water. The antibacterial maca spray has the advantages that inhibition effects can be realized for hemolytic streptococcus, streptococcus pneumoniae, Escherichia coli bacteria, proteus vulgaris, salmonella paratyphi A, shigella dysenteriae, Escherichia coli, staphylococcus aureus, methicillin-resistant staphylococcus aureus, candida albicans, pseudomonas aeruginosa and the like, the antibacterial maca spray can be used for inhibiting the Escherichia coli, the staphylococcus aureus, the candida albicans and the like and has little skin irritation, and good wetting effects can be realized.

A composite medicine in the form of capsules contains mannosan peptide, which is prepared from the fermented substance of streptococcus hemolyticus-alpha-hemolysis through extracting and refining. Its preparing process and application are also disclosed. Its advantages are sure curative effect and low dosage.

The invention relates to a honeysuckle antibacterial composition which includes a honeysuckle extract, in effective dose, and auxiliary materials. The invention also discloses a preparation method and an application of the composition. The composition can effectively kill pathogenic bacteria, and has excellent killing effects on pathogens, such as staphylococcus aureus, escherichia coli, streptococcus hemolyticus and the like, which cause skin inflammation, antibacterial rate being not less than 60%. The honeysuckle antibacterial composition has excellent biocompatibility, is low in cytotoxicity and is high in safety, has excellent anti-inflammation and sterilization effect, is wide in available range, and also can be applied to infants and women in lactation period.

The invention discloses incense for eliminating plague, which is prepared from Chinese atractylodes rhizome, dried duckweed, dahurian angelica root, argyi leaf, realgar, nard, kaempferia galamga, nutgrass galingale rhizome and sweet wormwood. The incense can dispense fragrance so as to sterilize air, purify filth, clear away heat and toxic material such as influenza virus, encephalitis B virus and the like, kill bacteria such as tubercule bacillus, Klebs-Loeffler bacillus, streptococcus hemolyticus, and the like. After the incense is fired, the generated smoke has no harm and toxic and side effects to persons and livestock, the smelt is pure and mild, no peculiar smell is emitted, and the incense can be used for prevention of infectious diseases, evil influence, epidemic virus, pestilence, arashiyama and influenza virus and sterilization in stations, schools, hospitals, hotels and large cultivation farms.

The invention discloses lactobacillus harbinensis and application thereof. The lactobacillus harbinensis (M1) has a preservation number of GDMCC No.60305, and is preserved in guangdong culture collection center. The invention provides the application of the lactobacillus harbinensis as probiotics. The lactobacillus harbinensis is sensitive to antibiotics of ampicillin, tetracycline, chloramphenicol and the like, the safety meets the requirements of EFSA, and supernate of the lactobacillus harbinensis has significant effects of inhibiting listeria monocytogenes, streptococcus hemolyticus, staphylococcus aureus and the like. The invention further discloses the application of the lactobacillus harbinensis in fermented soymilk; a strain is used for fermenting the soymilk individually, the acid-producing capability is high, a viable count is large, the contents of substances with beany flavors are reduced or even zero, the contents of fermented milk characteristic aroma components are obviously improved, and a sensory flavor of the fermented soymilk is greatly improved, so that the lactobacillus harbinensis is an optimized starter of matrices, which are rich in plant oligosaccharide, ofsoybeans and the like.

The invention discloses application of cyclocarya paliurus leaves or extract thereof in preparation of an antibacterial agent. Experiment proves that the cyclocarya paliurus aqueous extract has a killing effect on escherichia coli, staphylococcus aureus, staphylococcus epidermidis, staphylococcus saprophyticus, streptococcus pneumoniae, hemolytic streptococcus A, hemolytic streptococcus B and lactobacillus acidophilus; and especially, has a killing effect on various drug-resistant bacteria such as escherichia coli ATCC25922, escherichia coli CMCC44155, streptococcus pneumoniae ATCC6303, hemolytic streptococcus A and hemolytic streptococcus B. The killing capability is increased along with the increase of concentration, and the bactericidal capability is obviously stronger than that of a reference product Pu'er tea water extract. Therefore, the cyclocarya paliurus leaves or the extract thereof has a good application prospect in preparation of antibacterial agents.

The invention discloses a phage-containing spray type environment disinfectant as well as a preparation method and an application thereof, which belongs to the technical field of biological disinfection, the effective components of the disinfectant comprise an escherichia coli bacteriophage CCTCC NO.M2016539, a staphylococcus aureus bacteriophage CCTCC NO.M2016535 and a hemolytic streptococcus bacteriophage CCTCC NO.M2019227, wherein the ratio of the effective components is 1: 1: 1; when the disinfectant with the titer larger than or equal to 1*10<10>pfu / ml is sprayed in the living environmentaccording to the dosage of 50-100 ml / m<3>, the disinfectant has a broad-spectrum bactericidal effect on common pathogenic bacteria and is high in specificity, a SM buffer solution and cane sugar canprotect the stability of the titer of bacteriophage, and long-term use and storage of the disinfectant are facilitated; the purifying effect on living environment is good, safe and non-toxic.

The invention provides the application of quercetin in the preparation of medicine for preventing or treating diseases caused by influenze virus, adenovirus, staphylococcus aureus and / or streptococcus hemolyticus and the like, and a pharmaceutical composition thereof. The pharmaceutical composition has the activity for resisting the influenze virus being equivalent to oseltamivir.

The invention belongs to the novel technical field of medicaments, and relates to a novel dosage form of amoxicillin used for treating: 1, upper respiratory tract infections, such as tympanitis, nasosinusitis, pharyngitis and tonsillitis resulted from streptococcus hemolyticus, streptococcus pneumoniae, staphylococcus or haemophilus influenzae; 2, genitourinary tract infections resulted from escherichia coli,proteus mirabilis or enterococcus faecalis; 3, skin and soft tissue infection resulted from streptococcus hemolyticus, staphylococcus bacteria or escherichia coli; 4, lower respiratory tract infections, such as acute bronchitis and pneumonia, resulted from streptococcus hemolyticus, streptococcus pneumoniae, staphylococcus bacteria or haemophilus influenzae; 5, acute simple gonorrhea; and 6, typhoid, typhoid bacteria-carrier, leptospirosis and the like. The novel dosage form is characterized in that the stomach floating tablet is hydrated into gel on the surface to expand the volume thereof after contacting with the gastric juice at the body temperature because of a plurality of hydrophilic macromolecular materials contained. Meanwhile, the weight of the tablet is less than the buoyant force of the gastric juice to float the tablet on the gastric juice and prolong the retention time in the stomach.

The invention relates to a preparation method of a toilet paper product containing graphene, and discloses a process of medicinal plant papermaking. The process comprises the steps of screw extrusionprocessing, cooking, washing, papermaking, paper product modification, etc. The preparation method is characterized in that medicinal plant is used as a raw material to prepare fibers, and ultrasonicand ethanol methods are used in combination. The prepared paper product containing graphene has good softness and high strength, and no harmful chemicals are introduced during the whole process, so that the hygienic characteristic of the fibers is ensured; the prepared paper product has health care functions and effectively inhibits bacteria and resists bacteria; and since a graphene dispersion isadded during a pulping process, the obtained product has good antibacterial properties, and in particular, plays effective inhibiting and killing effects on staphylococcus aureus, escherichia coli, and streptococcus hemolyticus.

The oral digestible film includes water soluble polymer, enzyme and / or polypeptide in effective antibacterial amount. Test shows the oral digestible film can kill Staphylococcus aureus, colibacillus, Candida albicans, Streptococcus hemolyticus, Klebsiella pneumoniae and other disease-causing bacteria. The rat vagina mucous membrane stimulating reaction test shows the highest total integration value being zero, and the oral digestible film has no any stimulation to oral cavity mucous membrane. Halimeter test proves its oral cavity protecting effect in four hours and capacity of protecting fresh oral smell. The oral digestible film is used in killing harmful bacteria in oral cavity, keeping clean oral cavity and preventing and treating oral diseases.

The invention relates to an excellent microorganism strain for space mutation, in particular to a good microorganism strain for space mutation, and the preparation method and the application thereof. The discoveries that the bump of a cancer patient infected by hemolytic streptococcus can subsidise naturally and the hemolytic streptococcus and the preparation thereof have a certain of biological activities are made is as early as in the 19th century. In the invention, the special environment of the outer space is utilized to deliver the strains to the outer space through carrying the 22nd recoverable experimental science satellite, the 22nd recoverable experimental science satellite safely returns after traveling for a certain period of time, the suppresormutation strains are eliminated, the forward mutation strains with excellent features are selected out for re-screening, and ultimately the excellent microorganism strains with vigorous life, quick growth speed, high fermentation yield, short fermentation period, high polypeptide content, stable hereditary property, strong productive adaptability and space cumulative effect. The microorganism strain can be applied to the strains for industrialization production, and has the advantages of high output, wide application range, low cost and high effective component content.

VACCIN AGAINST GROUP A BETA HEMOLYTIC STREPTOCOCCUS AND RESPECTIVE PROCESS FOR OBTAINING THEREOF, which predicts the production of recombinant protein cloned from the gene emm5, which contains a sequence of oligonucleotides corresponding to 52 and / or 87 amino acid residues capable of protection, isolated after the sequential molecular identification of the epitopes from the M protein carboxy-terminal region, differing in 01 amino acid residue, identified by antibodies and T lymphocytes of health human beings and of patients carriers of rheumatic fever, capable of generating a protective response by antibodies depending on the T lymphocytes; prevention of the development of the autoimmune disease by the selected epitope was evaluated in vitro with T lymphocytes from the cardiac tissue of patients with lesions arising out of rheumatic fever.

The invention provides a traditional Chinese medicine composition for treating tonsillitis. The traditional Chinese medicine composition comprises the following components, by weight: 5 to 30 parts ofhoneysuckle, 5 to 30 parts of houttuynia cordata, 5 to 20 parts of the root of common peony, 5 to 15 parts of argy wormwood leaf and 5 to 15 parts of mint. Experiments show that the traditional Chinese medicine composition provided by the invention is capable of obviously inhibiting beta hemolytic streptococcus and staphylococcus aureus and inhibiting mouse ear swelling caused by xylene. Iin addition, clinical experiment results also show that the traditional Chinese medicine composition or the preparation thereof has a remarkable effect in the aspect of treating tonsillitis.

The invention relates to paper prepared from wastes of agriculture and forestry and a preparation method for the paper. A disclosed process for preparing fibers from the wastes of agriculture and forestry comprises the procedures of steaming treatment, screw extrusion, mechanical pulping, graphene dispersion solution modifying and the like. According to the method, the wastes of agriculture and forestry serve as a raw material for preparing the fibers, and screw extrusion and mechanical pulping are combined, so that the fibers prepared by the method are high in yield and low in Kappa number; and no harmful chemical is introduced in the entire process, so that the hygienic characteristic of the fibers is ensured. Due to a graphene dispersion solution added during pulping, the obtained product has relatively good conductive function and antibacterial performance and particularly plays a role in effectively inhibiting and killing staphylococcus aureus, escherichia coli and streptococcus hemolyticus.

The invention belongs to the field of microorganisms, and particularly relates to a microbial antibacterial deodorant preparation, and a preparation method and application thereof. The microbial antibacterial deodorant preparation disclosed by the invention is prepared from functional bacteria including bacillus aceticus, halomonas, lactobacillus casei and saccharopolyspora chrysosporium; and theratio of colony count of the acetobacter aceticus to the halomonas to the lactobacillus casei to the saccharopolyspora chrysosporium is (3-7):(3-8):(1-6):(0.5-2). The microbial preparation disclosed by the invention does not contain chemical additives; all functional bacteria surviving in the microbial preparation are dominant bacteria; a microorganism interspecific competition principle is utilized; the killing effect of metabolic products of the strains on pathogenic microorganisms is also achieved; the microbial antibacterial deodorant preparation shows excellent antibacterial and virus inactivation effects, the antibacterial effects on pseudomonas aeruginosa, B hemolytic streptococcus, shigella dysenteriae, staphylococcus aureus and escherichia coli all reach 93% or above, and the inactivation rate on influenza A H1N1 viruses reaches 99% or above.

The invention relates to an excellent microorganism strain for space mutation, in particular to a good microorganism strain for space mutation, and the preparation method and the application thereof. The discoveries that the bump of the cancer patient infected by hemolytic streptococcus can subsidise naturally and the hemolytic streptococcus and the preparation thereof have a certain of biological activities are made is as early as in the 19th century. The invention utilizes the special environment of the outer space to deliver the strains to the outer space through carrying the 21st recoverable experimental science satellite, the 21st recoverable experimental science satellite safely returns after traveling for a certain period of time, the suppresormutation strains are eliminated, the forward mutation strains with excellent features are selected out for re-screening, and ultimately the excellent microorganism strains with vigorous life, quick growth speed, high fermentation yield, short fermentation period, high polypeptide content, stable hereditary property, strong productive adaptability and space cumulative effect. The microorganism strain can be applied to the strains for industrialization production, and has the advantages of high output, wide application range, low cost and high effective component content.

A number of β-hemolytic streptococci polynucleotides and polypeptides, particularly Streptococcus pyogenes polypeptides and polynucleotides, are described. Two or more of the polypeptides of the invention can be formulated for use as immunogenic compositions. Also disclosed are methods for immunizing against and reducing infection caused by β-hemolytic streptococci.