HTNV vaccine based on VSV vector as well as preparation method and application of HTNV vaccine

A vaccine and vector technology, applied in biochemical equipment and methods, DNA/RNA vaccination, vectors, etc., can solve the problems of low ability to activate cellular immunity, low vaccination compliance, weak ability to induce neutralizing antibodies, etc., to improve Effects of virus titer, low infectivity, and strong wildness

Pending Publication Date: 2021-12-28
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Abstract
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  • Claims
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AI Technical Summary

Benefits of technology

The technical effects described by this patented inventions are that it has several advantages over existing methods such as live attenuated influenza strains used during flu seasonings with potential safety concerns due to their ability to infect humans through contact. It also includes various techniques like DNA shuffling technology, cloning vector systems, and transposons carrying foreign sequences from other organisms. This makes them ideal tools for creation of future vaccinacies against emerging diseases caused by these pathogens.

Problems solved by technology

This patented technical problem addressed by this patents relates to developing efficient and effective methods or agents against severe viruses like hANTAV/TRW, such as those caused by other members of Orthomyxovirus genus called Tylosomus sinensis (TTX).

Method used

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  • HTNV vaccine based on VSV vector as well as preparation method and application of HTNV vaccine
  • HTNV vaccine based on VSV vector as well as preparation method and application of HTNV vaccine
  • HTNV vaccine based on VSV vector as well as preparation method and application of HTNV vaccine

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Experimental program
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Embodiment 1

[0062] A kind of preparation method of the HTNV vaccine based on VSV carrier, concrete process is as follows:

[0063] 1. Construction of rVSVΔG-HTNV M(I532K / S1094L / ΔC6)-GFP plasmid (Δ represents the deletion of this part of the gene):

[0064] (1) Construction of pCAGGS-HTNV M(I532K) vector

[0065] The HTNV M used in this experiment is the HTNV 76-118 standard strain virus gene sequence, which was synthesized after codon optimization by Nanjing GenScript Company to obtain the plasmid pUC-opti GPC, the nucleotide of opti GPC in the plasmid pUC-opti GPC The acid sequence is shown in SEQ ID NO.1;

[0066] Then, using the pUC-opti GPC plasmid as a template, point mutations were performed at the 532 and 1094 amino acid positions, and corresponding mutation primers were designed, specifically including the following primers:

[0067] Upstream primer opti GPC-F-infu (gene sequence shown in SEQ ID NO.2), downstream primer GPC-R-infu (gene sequence shown in SEQ ID NO.3), downstream...

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Abstract

The invention relates to the technical field of vaccine preparation, and particularly discloses an HTNV vaccine based on a VSV vector as well as a preparation method and application of the HTNV vaccine, and the HTNV recombinant VSV vaccine with immunogenicity is obtained by inserting a nucleotide sequence of HTNV envelope glycoprotein into a recombinant VSV vector plasmid to construct a recombinant plasmid and then packaging and processing the recombinant plasmid. The HTNV vaccine prepared by the invention provides a novel candidate vaccine for preventing and treating hemorrhagic fever with renal syndrome.

Description

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Claims

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Application Information

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Owner FOURTH MILITARY MEDICAL UNIVERSITY
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