Unlock instant, AI-driven research and patent intelligence for your innovation.

High growth influenza virus

An influenza virus, influenza B virus technology, applied in the field of use, can solve problems such as inability to guarantee protection

Pending Publication Date: 2021-12-28
BLUESKY IMMUNOTHERAPIES GMBH +1
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The frequent antigenic changes of the virus further contribute to the high number of deaths, as even annual vaccination is not guaranteed to provide protection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High growth influenza virus
  • High growth influenza virus
  • High growth influenza virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0296] Purpose:

[0297] The 6:2B / Thuringia / 02 / 06:B / Murmansk / 3 / 2010delNS1 point mutant was tested for growth in a growth curve assay to identify mutations that resulted in improved virus growth.

[0298] method

[0299] Generation of 6:2 recombinant viruses with specific amino acid changes

[0300] To generate amino acid changes in internal genes, point mutations were performed in plasmids containing the gene of interest by subjecting each plasmid to site-directed mutagenesis using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA). Generation of 6:2 reassortant viruses by reverse genetics. Six pHW2000 derivatives containing fragments PB2, PB1, PA, NP, M, ΔNS1 derived from B / Thuringia / 02 / 06 (a B / Jiangsu / 10 / 03-like virus from the B Yamagata lineage) (plasmid) and a protein expression plasmid encoding influenza A PR8 NS1 (pCAGGS-NS1(SAM)) co-transfected into Vero cells with a pHW2000 derivative plasmid containing the HA and NA genes from B / Murma...

Embodiment 2

[0330] Purpose:

[0331] The 6:2B / Thuringia / 02 / 06:B / Phuket / 3073 / 2013delNS point mutant was tested in a growth curve assay to identify mutations that lead to improved virus growth.

[0332] method:

[0333] Generation of 6:2 recombinant viruses with specific amino acid changes

[0334] To generate amino acid changes in internal genes, point mutations were performed in plasmids containing the gene of interest by subjecting each plasmid to site-directed mutagenesis using the QuikChangeLightning Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA). Generation of 6:2 reassortant viruses by reverse genetics. Six pHW2000 derivatives containing fragments PB2, PB1, PA, NP, M, ΔNS1 derived from B / Thuringia / 02 / 06 (a B / Jiangsu / 10 / 03-like virus from the B Yamagata lineage) (plasmid) and a protein expression plasmid encoding influenza A PR8 NS1 (pCAGGS-NS1(SAM)) were co-transfected into Vero cells with a pHW2000 derivative plasmid containing the HA and NA genes from B / Puji / 3073 / 2013 ...

Embodiment 3

[0361] Purpose:

[0362] The 6:2 A / IVR-116:A / Hong Kong / 4801 / 2014 delNS1 point mutant was tested for growth in a growth curve assay to identify mutations that lead to improved virus growth.

[0363] method:

[0364] Generation of 6:2 recombinant viruses with specific amino acid changes

[0365] To generate amino acid changes in internal genes, point mutations were performed in plasmids containing the gene of interest by subjecting each plasmid to site-directed mutagenesis using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA). Generation of 6:2 reassortant viruses by reverse genetics. A laboratory virus containing the PB2, PA, NP, M, and NS genes from A / Puerto Rico / 08 / 1934 and PB1 from A / Texas / 1 / 1977, derived from A / IVR-116, will be Six pHW2000 derivatives (plasmids) of fragments PB2, PB1, PA, NP, M, and ΔNS1 of strain A virus) and a protein expression plasmid (pCAGGS-NS1 (SAM)) encoding influenza A PR8 NS1 combined with those from A / HK / 480...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention provides high growth influenza reassortant virus and high growth influenza reassortant virus vectors comprising amino acid modifications in the PB2, PB1, M1 and / or NS2 proteins which exhibit highly increased growth rates compared to unmodified influenza virus. Further provided are pharmaceutical compositions comprising reassortant virus and viral vectors comprising said modifications and their use for vaccination purposes.

Description

[0001] The present invention provides recombinant influenza viruses and influenza virus vectors comprising amino acid modifications in the PB2, PB1, M and / or NS2 proteins, which exhibit highly increased growth rates compared to unmodified influenza viruses. [0002] Also provided are pharmaceutical compositions comprising reassortant viruses containing said modifications and viral vectors and uses thereof for vaccination purposes. Background technique [0003] Epidemic and pandemic diseases caused by viral diseases are still claiming human life and affecting the global economy. Influenza has resulted in millions of lost workdays, hundreds of thousands of hospitalizations worldwide (Couch 1993, Ann.NY.Acad.Sci 685; 803), tens of thousands of excess deaths (Collins & Lehmann 1953 PublicHealth Monographs 213:1; Glezen 1982 Am.J.Public Health 77:712) and billions of euros in medical costs (Williams et al. 1988, Ann.Intern.Med.108:616). When healthy adults are vaccinated, currentl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/145
CPCA61K39/12C12N2760/16234C12N7/00C12N2760/16221C12N2760/16222C12N2760/16251
Inventor T·穆斯特尔A·阿斯佩隆德M·沃尔舍克
Owner BLUESKY IMMUNOTHERAPIES GMBH