An objective lens with large field of view, high resolution and wide band
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A high-resolution, wide-band technology, applied in the optical field, can solve the problems of narrow application scenarios, limited working band, small field of view, etc., to achieve the effects of wide application scenarios, improved imaging throughput, and high numerical aperture
Active Publication Date: 2022-07-05
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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The objective lens has a simple structure and can be used for two-photon imaging, but the working band is very limited, only 30nm, and can only be used for fluorescence imaging of a small number of specific markers, and the application scene is narrow
Chinese patent CN108873288 proposes an objective lens structure with high numerical aperture and large field of view. The objective lens is 0.75≤NA≤1. From the accompanying drawings 7 and 9 of the patent, it can be seen that the typical value of the field of view of the objective lens is 2.2mm, compared with The conventional objective lens has been significantly improved, but its field of view is still small, which is difficult to meet the imaging requirements of large-scale samples, and the working band is 500-800nm, which does not cover the common band of two-photon imaging
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[0059] In order to make the purpose, technical solutions and advantages of the present application more clearly understood, the present application will be described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application, but not to limit the present application.
[0060] It should be noted that if there are descriptions involving "first", "second", etc. in the embodiments of the present invention, the descriptions of "first", "second", etc. are only used for description purposes, and should not be understood as instructions or Implicit their relative importance or implicitly indicate the number of technical features indicated. Thus, a feature delimited with "first", "second" may expressly or implicitly include at least one of that feature. In addition, the technical solutions between the various embodiments can be combined with...
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Abstract
The invention discloses an objective lens with a large field of view, high resolution and a wide band, comprising nineteen spherical lenses arranged on the same optical axis, and the nineteen lenses sequentially include: a first lens and a second lens from the object side to the image side. , third lens, fourth lens, fifth lens, sixth lens, seventh lens, eighth lens, ninth lens, tenth lens, eleventh lens, twelfth lens, thirteenth lens, tenth lens Four lens, fifteenth lens, sixteenth lens, seventeenth lens, eighteenth lens, nineteenth lens. Compared with the similar objective lens proposed before, this objective lens has higher resolution, larger imaging field of view, and wider working band, which can meet the high-resolution imaging requirements of visible light and near-infrared two-photon large field of view.
Description
technical field [0001] The invention belongs to the field of optical technology, in particular to an objective lens with a large field of view and a high resolution and a wide band. Background technique [0002] As a key device in the microscope, the microscope objective lens images the sample to the image plane with a specific field of view, focal length, and numerical aperture (NA) for observation or detection. Restricted by the principle of optical imaging, the field of view and resolution of the objective lens are mutually restricted. The numerical aperture of the objective lens determines the resolution. Usually, the effective imaging field of view of the objective lens with an NA of 0.5 is about 1mm. When imaging some large samples, the method of field splicing is often adopted, the imaging throughput is low, and the imaging speed is slow. For example, high-resolution imaging of mouse whole brain slices may take tens of days. In order to solve this problem, in 2016, ...
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