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Device and system for rapidly analyzing and identifying sample as well as application and method of device and system

A sample and fast technology, which is applied to mass spectrometry analysis, a device for rapid analysis and identification of samples, and the system application field of implementing the device, can solve the problems of time-consuming, inability to accurately reflect characteristic information, cumbersome processing process, etc. The effect of usage, improving timeliness, and improving accuracy

Pending Publication Date: 2022-01-11
INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Limited by this factor, the compound ion mass spectrometry signals collected by sampling methods such as puncture, section surface desorption, and even shallow tissue ablation cannot accurately reflect the deep and overall characteristic information of the sampled tissue.
In addition, as previously mentioned, the sample handling process for preparing tissue sections is cumbersome and time-consuming

Method used

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  • Device and system for rapidly analyzing and identifying sample as well as application and method of device and system
  • Device and system for rapidly analyzing and identifying sample as well as application and method of device and system
  • Device and system for rapidly analyzing and identifying sample as well as application and method of device and system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1: Rapid identification of gene mutation status in glioma tissue

[0051] In this example, the purpose is to prove that atmospheric pressure mass spectrometry analysis of thin layers of samples derived from glioma pathological tissue can be used for rapid identification of tumor gene mutation status.

[0052] Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

[0053] method:

[0054] Preparation of shaped templates: A polyvinyl chloride film with a size of 1.5 cm×3.5 cm and a thickness of 200 μm was used as a shaped material for preparing thin slices of biological samples. Use a high-precision cutting plotter to carve a rectangular groove (the size of the rectangular groove is 10mm×15mm) on the polyvinyl chloride film. Remove the protective film on the back of the polyvinyl chloride film with the rectangular groove engraved, stick it on a glass slide (25mm×75mm in size), and form a shaping template for ...

Embodiment 2

[0064] Example 2: Quantitative methodological investigation of sample thin-layer array for rapid analysis of biomarkers in brain tissue

[0065] In this example, the purpose is to prove that atmospheric pressure mass spectrometry analysis of the prepared sample thin-layer array can be used for batch analysis, specifically, quantitative standard curve, repeatability and other methodological investigations and high-throughput analysis. On the other hand, in this embodiment, the liquid biological sample is also prepared as a sample thin layer, specifically, the liquid biological sample is rat brain tissue homogenate.

[0066] method:

[0067] Internal standard compound standard solution preparation: Accurately weigh 4 mg of deuterated 2-hydroxyglutaric acid disodium salt standard product (molecular formula: C5H3D3Na2O5, accurate molecular weight is 195.0193), and prepare a total of 4 ml of 1 mg / ml internal standard compound stock solution. Reserve in a 5ml storage bottle. Prepa...

Embodiment 3

[0079] Example 3: High-throughput quantitative analysis of biomarkers in tumor tissue using thin-layer arrays of samples

[0080] In this example, the purpose is to prove that atmospheric pressure mass spectrometry analysis of the thin-layer 3×8 array of tumor tissue samples prepared in batches can be used for high-throughput quantitative analysis of biomarkers in tumor tissue.

[0081] method:

[0082] In this embodiment, the preparation of a 3×8 array of thin layers of tumor tissue samples, the parameter settings of the atmospheric pressure ion source (AFADESI) and the mass spectrometer are the same as those in Embodiment 2.

[0083] Specifically, the thin layer 3×8 array of tumor tissue samples to be tested was prepared: 24 glioma tissue samples were taken from a -80°C refrigerator and placed at -20°C. One tumor tissue sample was taken out each time, placed on the edge of the rectangular groove of the shaped template at room temperature, the tumor tissue was crushed with a...

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Abstract

The invention belongs to the technical field of analysis and detection, and relates to a device and a system for rapidly analyzing and identifying a sample as well as application and a method thereof. The device is characterized in that the device comprises a sample dispersion module used for rapidly preparing a sample into a uniformly mixed sample thin layer; a desorption module which is used for releasing aerosol sample particles from the surface of the sample thin layer; a transmission module which is used for transmitting the generated aerosol sample particles to an analyzer; an ionization module which is used for ionizing the transmitted aerosol sample particles to generate gaseous sample ions; and an analyzer which is used for detecting the gaseous sample ions and acquiring data related to the overall characteristic information of the sample. The invention also relates to a system application and a method for implementing the device. Figure 1 in the drawings of the specification is taken as the abstract drawings of the invention.

Description

technical field [0001] The invention relates to mass spectrometry, in particular to a device, system, application and method for rapid analysis and identification of samples. The present invention takes into account the fact that the distribution of compound molecules representing sample characteristics is usually not uniform in the sample, and provides a device for quickly preparing a thin layer of a representative sample. Or quantitatively detect the compound molecules representing the overall characteristics of the sample, so as to realize the rapid analysis and identification of the sample. The invention also provides system applications and methods for implementing the device. [0002] The invention belongs to the field of analysis and is applicable to all fields requiring timeliness for analysis and identification of samples. For example, in the field of biomedicine, rapid detection of biomarkers in pathological tissues under specific circumstances. Similarly, the pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62G01N27/68G01N1/28
CPCG01N27/62G01N27/68G01N1/28
Inventor 厉欣再帕尔·阿不力孜贺玖明张瑞萍回相宜王子璇
Owner INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
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