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Application of roxadustat in medicine for treating migraine and medicine containing roxadustat

A technology for roxadustat and migraine, which is applied in the fields of medicine and special medical food, can solve the problems such as no research on the use of anti-migraine diseases, and achieve the effects of improving the state of central sensitization and inhibiting pain sensitivity.

Active Publication Date: 2022-02-01
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention mainly focuses on the role of roxadustat in the treatment of migraine. Existing studies have shown that roxadustat has anti-inflammatory, anti-oxidation and other functions, but no research has shown that it has anti-migraine diseases.

Method used

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  • Application of roxadustat in medicine for treating migraine and medicine containing roxadustat
  • Application of roxadustat in medicine for treating migraine and medicine containing roxadustat
  • Application of roxadustat in medicine for treating migraine and medicine containing roxadustat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Roxadustat improves the pain sensitivity of migraine model mice

[0030] 1. Grouping of mice

[0031] Twenty-four 8-week-old male wild-type C57BL / 6J mice of SPF grade, weighing about 22g, were randomly divided into 3 groups, namely normal saline control group (NS group), model group (NTG group), and roxadustat treatment group (NTG+Rox group). According to the guidance of the literature, the present invention established a mouse migraine model by repeated intraperitoneal injection of 10 mg / kg of nitroglycerin solution, injected once every other day, a total of 5 times, and continued for 11 days. The model group and the roxadustat treatment group were On the 3rd, 5th, 7th, 9th, and 11th day of the experiment, 10 mg / kg nitroglycerin solution was injected intraperitoneally, and the same amount of normal saline was injected intraperitoneally (ip) in the control group. In addition, the mice in the roxadustat treatment group were fed with roxadustat-containing food...

Embodiment 2

[0036] Example 2 Roxadustat improves the central sensitization state of migraine mice

[0037] 1. Protein extraction

[0038] After the experiment, the mice were euthanized, and the TNC tissue of the mouse brain was collected. After fully grinding the tissue of the caudate nucleus of the trigeminal nerve with protein lysate, centrifuge at 12000r / min at 4°C for 10min, take the supernatant, and transfer it to a -20°C refrigerator for storage.

[0039] 2. Western blot

[0040] 1) The total protein concentration was determined by the BCA method, 50 μg of total protein was quantified, and the loading volume of the protein sample was calculated.

[0041] 2) Use SDS-polyacrylamide gel electrophoresis at a constant voltage of 124V for about 90 minutes.

[0042] 3) The western blot was transferred to the NC membrane under a constant voltage of 100V.

[0043] 4) Obtain the target band according to the molecular weight of the protein, and block it with 5% skimmed milk at room tempera...

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Abstract

The invention discloses an application of roxadustat in a medicine for treating migraine and the medicine containing the roxadustat, and relates to the technical field of medicines, wherein the migraine is chronic migraine. The used roxadustat can significantly inhibit nitroglycerin-induced pain sensitivity related to mouse migraine and improve the central sensitization state of the trigeminal nerve spine tail nucleus part, the new application of the roxadustat in the aspect of migraine resistance can expand the research of the roxadustat in the field of medicine, and a new theoretical direction is provided for clinical application.

Description

technical field [0001] The invention relates to a new application of roxadustat, specifically the application of roxadustat in medicine for treating migraine and the medicine containing roxadustat, belonging to the technical field of medicine and food for special medical treatment. Background technique [0002] Migraine is a common primary clinical disease, manifested as recurrent headache, unilateral throbbing, often accompanied by nausea, photophobia, phonophobia, general malaise and other symptoms. According to statistics, genetic, environmental, hormonal and other factors affect the first attack of migraine. The incidence rate of migraine in my country is 9.3%, which seriously affects the physical and mental health and quality of life of patients, and brings huge economic burden to families and society. To date, the pathogenesis of migraine is unclear. Numerous studies have shown that central sensitization increases the frequency of migraine attacks and even maintains ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/472A61P25/06
CPCA61K31/472A61P25/06
Inventor 韩际宏杨代刚杨潇潇段亚君陈元利张爽高永耀
Owner HEFEI UNIV OF TECH
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