69 results about "Nitroglycerol" patented technology

The present invention is organ and tissue preservation solutions that provide improved viability of an organ such as a heart or lung, or portion of the organ, for storage and transplantation. In particular, a solution contains trehalose, magnesium sulfate, calcium chloride, heparin, dextran, nitroglycerin, adenosine, L-arginine, allopurinol, reduced glutathione, db-cylic AMP and potassium phosphate.

An improved preservation solution is described, which is intended for the preservation of organs and tissues, or parts thereof, from humans and animals. The improved preservation solution contains calcium, at least one colloidosmotically active substance, and nitroglycerin. Also described is a method for preserving organs and tissues, or parts thereof, from humans and animals in the improved preservation solution.

The present invention relates to compounds for treatment that protects the endothelium, prevents pathologic thrombus formation in the microcirculation and preserves platelet number and function and thus may be related to treatment or prevention of ischemic events in patients with cardiovascular disease. The present invention is particularly useful for patients having or being at increased risk of development of an ischemic event such as an acute myocardial infarction and / or no-reflow phenomena and / or ischemia-reperfusion injury by administration of agent(s) modulating and / or preserving endothelial integrity. The compounds may be administered in combination with standard treatment of acute cardiovascular ischemic events such as Platelet inhibitors such as aspirin (ASA), Thienopyridins, GPIIb / IIIa inhibitors), Parenteral anticoagulants such as unfractioned heparin (UFH), bivalirudin, enoxaparin, and fondaparinux, Verapamil, Adenosine, Sodium nitroprusside, Nitroglycerin, Epinephrine, Beta-blockers and surgical methods such as percutaneous coronary intervention (PCI), PCI with thrombus aspiration, PCI with stents.

The invention discloses a detecting method for accurate risk early warning and accurate drug use of cardiovascular and cerebrovascular diseases and a specific primer. The invention provides a specific primer for detecting SNP site of drug resistance of the cardiovascular and cerebrovascular diseases, which comprises a primer group 1 and a primer group 2; the primer group 1 is composed of primer pair 1-primer pair 13; a primer group 2 is composed of single stranded extended primer 1- single stranded extended primer 13; the method can efficiently detect and complete the accurate early warning of the attack of the cardiovascular and cerebrovascular diseases and the accurate drug use after being attacked, namely, four kinds of cardiovascular and cerebrovascular disease early warning with huge health risk and accurate drug use of four clinical common drugs (aspirin, nitroglycerin, warfarin, and clopidogrel) for prevention and treatment of cardiovascular and cerebrovascular diseases.

The present invention is related to the composition and methods of manufacture of orally-dissolvable, edible films as a vehicle for the non-invasive administration of nitroglycerin and / or other active drugs through the mucosal tissues of the oral cavity. The films include a water soluble film-forming polymer such as pullulan. Methods for producing the films are also disclosed.

The invention discloses a primer composition for guiding nitroglycerin medication and healthy drinking, a multiple gene detection kit and a use method of the kit, and the kit comprises the primer composition, a PCR buffer solution and a positive reference substance, and the PCR buffer solution comprises ultrapure water, an X solution, a 10*PCR (polymerase chain reaction) buffer solution, a PCR primer, a 25mM magnesium chloride solution and DNA polymerase, the primer composition comprise two forward and reverse amplification primers of different gene types on the 2 SNP sites of genes related to the nitroglycerin medication and healthy drinking and forward and reverse amplification primers capable of reflecting internal reference, the gene sequences of the primers are represented as SEQ ID NO.1-NO.8; the use method comprises the step of acquiring a sample and extracting nucleic acid, the step of performing the PCR reaction by using extracted nucleic acid as a template, and the final step of separating the sample through capillary electrophoresis. The primer composition has the advantages of being strong in specificity, high in accuracy, high in flux, strong in reliability, low in cost and free from false negative result.

The invention provides an oral instant membrane comprising edible nano fibers and a preparation method thereof, belonging to the fields of nano-biological medical materials and oral instant membranes preparation by utilizing a pneumoelectric spinning technology. The method comprises the following steps: preparing a protein fluid, polyvinylpyrrolidone, medicaments and a preservative according to a proportion to serve as a pneumoelectric spinning solution; uniformly mixing, and spraying from a spray needle; and controlling the gas flow velocity to be between 1 and 10L / min, voltage to be between 10 and 20KV, mass flow velocity of an injection pump to be between 0.1 and 1ml / h, and receiving distance to be between 5 and 15cm. The oral instant membrane can reach nanometer grade by utilizing the pneumoelectric spinning technology, and has the excellent characteristics of nano-biological materials; then, proper medicaments are added on the basis of the oral instant membrane according to different medical requirements, such as nitroglycerin, ibuprofen, loperamide hydrochloride, risperidone and other medicaments, so that a medical membrane made of an edible biological material can be obtained; and finally, the pneumoelectric spinning technology is utilized to improve the production efficiency, and the oral instant membrane is expected to be used in industrial production.

The invention discloses a method for detecting the precise medication of four kinds of common clinic cardiovascular medicine and a special primer. The invention provides the special primer for detecting four kinds of medicine-resistant SNP sites of the four kinds of common clinic cardiovascular medicine. The eight-SNP site detection relevant to aspirin resistance, nitroglycerin resistance, warfarin individual difference, clopidogrel resistance is efficiently completed through once experiment; the precise medication gene detection of aspirin, nitroglycerin, warfarin and clopidogrel is completed by once experiment; the experiment cost is reduced. The PCR primer designed according to each SNP site and the unique UEP primer have high specificity. The method has high application values on guiding the clinic precise medication of aspirin, nitroglycerin, warfarin and clopidogrel, and is suitable for popularization and application.

The invention relates to the field of molecular biology, and discloses a specific oligonucleotide probe for detecting an SNP (single nucleotide polymorphism) site of ALDH2 (alcohol dehydrogenase 2)gene, wherein the probe is capable of hybridizing with the 504th different genetype of the ALDH2 gene. The invention further discloses a specific primer pair for amplifying the ALDH2 gene when detecting the SNP site, and the primer pair can be used for specifically amplifying a target area containing a detection target site in the ALDH2 gene. The specific oligonucleotide probe and the specific primer pair provided by the invention can be used for detecting the ALDH2 genetype and providing information for people to learn individual alcohol metabolism detoxification ability and correctly use nitroglycerin.

The invention discloses an ALDH2 (aldehyde dehydrogenase 2) Gene polymorphism detection kit, having the advantages that detection sensitivity is high, the lowest detection limit is 7.8 pg DNA; the kitis simple to operate and allows point-of-care test without professional knowledge; the detection time is short, down to 45 min; the detection specificity is high, and the detection accuracy is very high since a specific fluorescent probe is used; nucleic acid extraction is not required, low-melting-point agarose is added, and PCR (polymerase chain reaction) is greatly avoided. When used with ParaDNA gene amplification detectors to carry out POCT (point-of-care test), the kit can be used to clinically provide guidance on the administration of nitroglycerin and the notification on the risk of alcohol drinking.

The invention discloses a nitroglycerin sublingual tablet and a preparation method thereof, and the preparation method comprises the following steps: dissolving a stabilizer in a 10% nitroglycerin ethanol solution, adding a filler for wet granulation, drying, size stabilization, mixing with a disintegrating agent, a glidant and a lubricant, and directly pressing powder to prepare the nitroglycerin sublingual tablet. Compared with the traditional wet granulation preparation method, the nitroglycerin sublingual tablet prepared by the method has the advantages that the stability of the product is greatly improved, the problem that the nitroglycerin sublingual tablet is easy to volatilize in the storage process is solved, and the safety problems of explosion and the like in the industrial production process are also solved. The product is suitable for preventing and rapidly relieving angina pectoris attack caused by coronary artery diseases.

The invention discloses a preparation method of glycerin trinitrate. The preparation method comprises the following steps: (1) nitration reaction; and (2) refining: A, purified water washing; B, waterremoval by using a drying agent; and C, activated carbon adsorption. The invention further provides glycerin trinitrate prepared by using the preparation method and an absolute ethyl alcohol solutionof glycerin trinitrate. The preparation method provided by the invention is high in yield, few in produced impurities and capable of effectively avoiding decomposition effects of reaction temperature, residual water and acids to glycerin trinitrate, reducing decomposition products of glycerin trinitrate and controlling the content of related substances in a storage process of glycerin trinitrateand the absolute ethyl alcohol solution thereof to make a glycerin trinitrate product more stable and ensure that the quality of the glycerin trinitrate product conforms to relevant regulations in a validity period.

The invention discloses a nitroglycerin micro-emulsion spray content assaying method. The method is an HPLC (High Performance Liquid Chromatography) method; the chromatographic conditions comprise: taking a Diamonsil C18 column (250 mm * 4.6 mm, 5 Mum) as a chromatographic column, taking acetonitrile-water at the ratio of 70:30 as a moving phase, the flow rate being 1.0 mL / min, the detection wave length being 215 nm, and the column temperature being 30 DEG C. The method is good in specificity, accuracy and day to day precision, and can be used for nitroglycerin micro-emulsion spray content assaying and quality monitoring.

A method and special-purpose primers used for detecting aspirin and nitroglycerin accurate administration are provided. The special-purpose primers for detecting aspirin and nitroglycerin drug resistance SNP loci are provided and include a primer group 1 and a primer group 2. The primer group 1 includes a primer pair 1, a primer pair 2, a primer pair 3 and a primer pair 4. The primer group 2 includes a single-chain extension primer 1, a single-chain extension primer 2, a single-chain extension primer 3 and a single-chain extension primer 4. An experiment proves that detection of a plurality of SNP loci related to aspirin and nitroglycerin drug resistance is completed efficiently by a one-time experiment, and the one-time experiment can complete aspirin and nitroglycerin accurate administration gene detection, thus reducing the experiment cost. The method and the primers have high application value for directing clinical accurate administration of aspirin and nitroglycerin and are suitable for popularization and application.

The invention relates to a specific process for continuous production of nitroglycerin raw materials and tablets. Through hard innovation working, unexpected progress is achieved in the aspect of continuous production of green nitroglycerin preparations, green, safe and risk-free synthesis is achieved in key API preparation, combined API and nitroglycerin tablets are integrally produced, and the process is disclosed for the first time in China, is encouraged by global supervision departments, and is the most trend of drug production.

Application of oxymatrine injection in antianginal drugs is characterized in that the oxymatrine injection is intravenous fluid prepared by diluting oxymatrine 2 mg / ml with 100 ml of 5% glucose; sitting administration is adopted that is referred to: seat height is adjusted through a lifting mechanism such that the forearm of one upper arm of a patient to take injection is on the same plane with the heart. The oxymatrine injection is the intravenous fluid prepared by diluting oxymatrine 2 mg / ml with 100 ml of 5% glucose; the sitting administration is adopted. The curative effect is 10% higher than that of a control group of nitroglycerin and isosorbide dinitrate.

The invention discloses an application of roxadustat in a medicine for treating migraine and the medicine containing the roxadustat, and relates to the technical field of medicines, wherein the migraine is chronic migraine. The used roxadustat can significantly inhibit nitroglycerin-induced pain sensitivity related to mouse migraine and improve the central sensitization state of the trigeminal nerve spine tail nucleus part, the new application of the roxadustat in the aspect of migraine resistance can expand the research of the roxadustat in the field of medicine, and a new theoretical direction is provided for clinical application.

Disclosed stable machinery lubricating oil is prepared from the following raw materials in parts by weight: 25-35 parts of sulfurized calcium alkyl phenolate, 0.5-1 part of nitroglycerin, 2-6 parts of trilinolein, 2-7 parts of sorbitol, 3-8 parts of phosphotriester and 5-10 parts of 42-type chlorinated paraffin. The lubricating oil provided by the invention has good high temperature properties with a dropping point more than 235 DEG C, the changed of the denseness of the lubricating oil is not obvious along with the change of temperature, and the lubricating oil is capable of satisfying the usage in a wide temperature scope and has the advantage of being not hardened at high temperature; the lubricating oil is low in cost, has good adhesion property compared with a composite aluminium phase, is improved in extreme pressure properties and load resistance, and therefore the lubricating oil is applicable to lubrication of high-temperature heavy-load apparatus running parts.

The invention discloses improved stable type lubricating oil. The improved stable type lubricating oil is prepared from the following raw materials in parts by weight: 25-35 parts of methyl acrylate, 0.5-1 part of nitroglycerin, 2-6 parts of trilinolein, 2-7 parts of sorbitol, 3-8 parts of phosphotriester and 5-10 parts of trimethyl decane. The lubricating oil disclosed by the invention has good high-temperature performance, a dropping point greater than 235 DEG C, and unobvious thickness changes with temperature changes, can satisfy use in a wide temperature range, and has the advantage of being not cured at a high temperature; meanwhile, the improved stable type lubricating oil is low in cost, has good adhesiveness in comparison with composite aluminum, and improves extreme pressure property and load resistance, and therefore, the lubricating oil disclosed by the invention can be used for lubricating operation parts of a high-temperature heavy-load device.

The invention relates to a green preparation method of nitroglycerin. On the basis of a micro-reactor of Nobel explosive company, according to the specific process of continuous production of nitroglycerin raw materials and preparations, unexpected progress is achieved in the aspect of continuous production of the nitroglycerin green preparation through innovation, green, safe and risk-free synthesis is achieved in key API preparation, the combined API and the nitroglycerin preparation are subsequently and integrally produced, and the method is initiated domestically, is encouraged by global supervision departments and is the most trend of drug production.

The invention relates to a nitroglycerin sublingual tablet for treating angina pectoris. According to the nitroglycerin sublingual tablet for treating the angina pectoris, a novel surfactant polyethyleneglycol stearate is utilized to increase the stability of the prepared nitroglycerin sublingual tablet and shorten disintegration time of the tablet in the oral cavity; and moreover, the nitroglycerin sublingual tablet has the advantages of being capable of avoiding first pass effect and convenient to store.

The invention discloses a method for detecting and pretreating nitroglycerin solution nitrate. The method comprises the following steps: naturally volatilizing the solvent of the detected sample under the condition of room temperature and normal pressure or room temperature or reduced pressure; dissolving purified water by residues; vibrating; placing and laminating; filtering; and applying the filtrate to a nitrate brown ring test, wherein the junction cannot show brown at once. Compared with the prior art, the invention has the advantages that the technology fills the gap of the medicinal nitroglycerin solution nitrate detection technology; the condition of combustion or explosion of nitroglycerin caused by the conventional nitrate detection method is eliminated successfully; the method is high in accuracy and high in stability; and the method is particularly suitable for large-scale production detection operation.

The invention discloses a primer for detecting polymorphism of an ADH1B gene and an ADH2B gene by a TaqMan-MGB probe method, and application of the primer, and belongs to the technical field of biological detection. Primers and probes as shown in SEQ ID NO:1 to NO:8 are provided. Compared with a sequencing method, the invention has the advantages that PCR products do not need to be subjected to a series of complex subsequent treatment, PCR amplification and detection are conducted synchronously, the whole detection process does not need cover-opening operation, the pollution risk caused by the PCR products is reduced, operation is simple and rapid, the detection time is greatly shortened and the detection cost is reduced. The primer can be used for clinically detecting the polymorphism of the ADH1B gene and the polymorphism of the ADH2B gene, and the detection results can be used for guiding clinical reasonable medication of nitroglycerin and healthy drinking.

The invention belongs to health care products in daily life products, and is mainly suitable for prevention, treatment and health care of patients with cardiovascular and cerebrovascular diseases, hypertension, pulmonary arterial hypertension, tumors and other diseases. The health care device is characterized in that solid nitroglycerin is arranged in a cylinder of a health care device body, nitric oxide gas generated by the solid nitroglycerin is stored and sealed in a lengthened hose and is autonomously controlled and used by people, and vitality of the human body is supplemented to the human body in an exogenous mode. The health care product has the beneficial effects as follows: the device can prevent and treat diseases such as cardiovascular and cerebrovascular diseases, hypertension,hyperlipidemia and hyperglycemia, pulmonary arterial hypertension and tumors, improve sleep quality, deepen learning memory, liver and lung protection, improve gastrointestinal functions and enhancesexual functions, is beneficial to immunity and exerting the anti-fatigue effect, also has the characteristics of being wide in adaptability, high in practicability, capable of maintaining heart and brain health and good in health care effect of reducing hypertension, hyperglycemia and hyperlipidemia, can effectively improves the attack condition of cardiovascular and cerebrovascular diseases through the application of the nitric oxide technology, and particularly prevents the cardiovascular and cerebrovascular diseases in a nitric oxide health-care mode.

The invention belongs to the field of research and development of a traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for preventing and treating a migraine disease as well as preparation method and an application of the medicine composition. The traditional Chinese medicine composition is prepared from medicines screened based on a traditional Chinese medicine clinical experience formula, namely a radix angelicae formula and is obtained by ethanol extraction according to the radix angelicae formula and purification and enrichment of macroporous adsorption resin. Main components of the traditional Chinese medicine composition are coumarins, phenolic acids and phthalide. Pharmacological experiments prove that the traditional Chinese medicine composition can be used for increasing the content of 5-HT in plasma of nitroglycerin-induced migraine model rats and the content of NO in brains of the rats, and reducing the content of calcitonin gene-related peptides in the brains of the rats and the content of endothelin in the brains of the rats, so that the migraine is prevented and treated.

The invention discloses a steel surface cleaning agent and a preparation method and application thereof. According to the steel surface cleaning agent and the preparation method and application thereof, the steel surface cleaning agent is prepared from, by weight, 10-20 parts of polyvinyl propylene, 5-9 parts of nitroglycerin, 2-4 parts of a polycarboxylic acid water-soluble dispersing agent, 7-11 parts of methylbenzene, 0.5-0.9 part of sodium stearate, 8-10 parts of fatty alcohol-polyoxyethylene ether, 2-8 parts of nanometer titania particles, 15-25 parts of cocamidopropyl betaine and 4-10 parts of dodecyl dimethyl betaine. According to the steel surface cleaning agent and the preparation method and application thereof, steel surface deoiling, derusting, phosphorization and passivation can be carried out synchronously, the treatment time is short, the phosphorization quality is good, the problems of serious pollution, long treatment time and large hurt to the human body existing in steel surface treatment are solved, zero-pollution emissions are achieved, and pollution to the environment is reduced.

Hypertrophic pyloric stenosis can be treated by suppressing contraction of the pyloric muscle by using a therapeutic agent or a treatment kit which comprises atropine sulfate as an anticholinergic agent and nitroglycerin or isosorbide nitrate as an NO donor as the active ingredients.

The invention relates to the technical field of medical detection, and concretely relates to a nitroglycerin ALDH2 gene polymorphism detection method and device. A detector is fixedly connected with an operation table and located above the operation table, a containing groove is formed in the operation table, a storage box is detachably connected with the operation table and located in the containing groove, a supporting rod is detachably connected with the storage box and located in the storage groove, an embedding ring is fixedly connected with the supporting rod and penetrates through the supporting rod, and a first rotating shaft is fixedly connected with one end of the supporting rod. A second rotating shaft is fixedly connected with the other end of the supporting rod, the two ends of a holding rod are rotationally connected with the first rotating shaft and the second rotating shaft respectively, and each stabbing rod is fixedly connected with the supporting rod. Reagent bottles are driven to leave the storage box when the stabbing rods are embedded with the reagent bottles and the holding rod is lifted, so that the reagent bottles are prevented from being broken and affecting the detection result in the detection process of detection personnel .

The invention discloses a simple and economical one-tube ALDH2 genotyping kit. The kit comprises the following components: a direct-amplification dye method qPCR premixed solution containing three primer groups, a positive plasmid control and a negative control. The kit is characterized in that the three primer groups are as follows: an ALDH2-F upstream primer is shown in SEQ ID NO: 1, an ALDH2-Rdownstream primer is shown in SEQ ID NO: 2, and an ALDH2-MR mutant downstream primer is shown in SEQ ID NO: 3. The kit disclosed by the invention adopts one-tube qPCR reaction, and an extraction stepof a sample is omitted, so that the test cost is greatly saved, and the pollution risk in the extraction process is avoided. The kit is accurate, rapid and low in cost, is particularly suitable for risk analysis of genetic factors such as drinking behavior, alcoholic cirrhosis and liver cancer, and is also a reference method for clinical nitroglycerin guiding medication.