Monatomic nano-enzyme as well as preparation method and application thereof

A nano-enzyme, atomic technology, applied in nanotechnology, nanotechnology, chemical instruments and methods, etc., can solve the problems of low single-atom loading and limit the application of single-atom nanozymes, and achieve simple and easy preparation methods and components. obtainable and controllable effects

Active Publication Date: 2022-02-08
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mass-selected soft deposition method and the atomic-level loading method both require specific expensive large-scale equipment and professional operators, and their single-atom loading is low
These two significant shortcomings limit its application in large-scale preparation of single-atom nanozymes.

Method used

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  • Monatomic nano-enzyme as well as preparation method and application thereof
  • Monatomic nano-enzyme as well as preparation method and application thereof
  • Monatomic nano-enzyme as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1: the preparation of Fe single atom nanozyme

[0053] 0.32g Fe(NO 3 ) 3 9H 2 O was dissolved in 20 mL of methanol to obtain 0.04 mol L -1 Ferric nitrate methanol solution (solution A); 11.9g Zn(NO 3 ) 2 ·6H 2 O was dissolved in 1000 mL of methanol to obtain 0.04 mol L -1 Zinc nitrate methanol solution (solution B); 20.1g methylimidazole was dissolved in 1000mL methanol to obtain 0.25mol L -1 Methanol solution of methylimidazole (Solution C). Put the above solution into figure 2 The feeding area of ​​the microfluidic reaction device shown.

[0054] First pass solution A and solution B into the transition chamber, stir and mix at a speed of 1000r / min, then pass the mixed metal ion solution and solution C into the microfluidic reaction chamber, and stir at a speed of 500r / min , keep the reaction at 60°C to obtain the conjugated supramolecule in the defect state, the reaction process is as follows image 3 shown. Set the flow rate of solution A to 0...

Embodiment 2

[0059] Embodiment 2: Preparation of Co single atom nanozyme

[0060] Prepare 0.125mol·L -1 Cobalt acetate N,N dimethylformamide solution (solution A) and 0.125mol·L -1 Zinc acetate N,N dimethylformamide solution (solution B) and 0.5mol L -1 1,1'-ferrocenecarboxylic acid N,N dimethylformamide solution (Solution C).

[0061] First pass solution A and solution B into the transition chamber, stir and mix at a speed of 1200r / min, then pass the mixed metal ion solution and solution C into the microfluidic reaction chamber, and stir at a speed of 800r / min , keep the reaction at 100°C to obtain the conjugated supramolecule in the defect state, the reaction process is as follows image 3 shown. Set the flow rate of solution A to 0 μL min -1 , 80 μL·min -1 , 160μL·min -1 , 240μL·min -1 , the four different flow rates correspond to the Co atom loadings from 0%, 4%, 8%, and 12% respectively; the corresponding solution B flow rate is set to 2000μL·min -1 , 1920μL·min -1 , 1840μL·...

Embodiment 3

[0066] Embodiment 3: the preparation of Mg single-atom nanozyme

[0067] Prepare 0.35mol·L -1 Magnesium chloride methanol solution (solution A), 0.2mol L -1 zinc chloride methanol solution (solution B) and 0.5mol L -1 4,4-bipyridine methanol solution (Solution C).

[0068] First pass solution A and solution B into the transition chamber, stir and mix at a speed of 800r / min, then pass the mixed metal ion solution and solution C into the microfluidic reaction chamber, and stir at a speed of 600r / min , keep the reaction at 60°C to obtain the conjugated supramolecule in the defect state, the reaction process is as follows image 3 shown. Set the flow rate of solution A to 0 μL min -1 , 80 μL·min -1 , 160μL·min -1 , 240μL·min -1 , four different flow rates correspond to the Mg atomic loads from 0%, 4%, 8%, and 12% respectively; the corresponding solution B flow rate is set to 2000μL·min -1 , 1920μL·min -1 , 1840μL·min -1 , 1760μL·min -1 ; The flow rate of solution C was...

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Abstract

The invention discloses monatomic nano-enzyme as well as a preparation method and application thereof. The monatomic nano-enzyme is prepared by the following steps that doped metal ions, main metal ions and organic monomers are coordinated in a microfluid reaction device to form conjugated supramolecules in a defect state; and the monatomic nano-enzyme is obtained by performing high-temperature pyrolysis on conjugated supramolecules in a defect state in an inert atmosphere. The organic monomer is coordinated to form a non-metal heteroatom doped carrier, metal ions and non-metal heteroatoms interact with each other, a partial reduction reaction occurs, and the metal ions are coated in a porous super-conjugated carbon carrier and exist in a monatomic form. The preparation method provided by the invention is suitable for preparing monatomic bodies of various metals, the preparation method is simple and easy to implement, and the prepared monatomic nano-enzyme has atomic-scale dispersion active sites. Experimental results show that the monatomic nano-enzyme prepared by the method has good peroxidase-like activity, and has a wide application prospect in the field of rapid detection of cysteine, ascorbic acid, glutathione and the like.

Description

technical field [0001] The invention relates to the technical field of functional materials, in particular to a single-atom nanozyme and its preparation method and application. Background technique [0002] At present, the catalytic effect of nanomaterials is closely related to the particle size of catalytic active sites. A large number of existing studies have shown that the smaller the size of nanozymes, the more significant their catalytic activity. In conventional nanoparticle-level catalytic materials, only the atoms on the outermost atomic layer have catalytic activity; and most of the atoms on it are in the interior, and almost all of them have no significant impact on the catalytic effect of nanomaterials. . Single-atom biomimetic enzyme refers to the material whose catalytic active site is a single metal atom. Its extremely small dispersion characteristics and extremely high catalytic activity (its atoms are all surface atoms, no internal atoms), greatly improve th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J23/80B01J23/755B01J23/75B01J23/745B01J23/72B01J23/60B01J23/46B01J23/22B01J23/18B01J23/06B01J21/10B22F9/30B22F1/07G01N21/31G01N33/68B82Y40/00B82Y30/00
CPCB01J23/745B01J23/80B01J23/75B01J21/10B01J23/06B01J23/18B01J23/468B01J23/60B01J23/22B01J23/72B01J23/755B22F9/30G01N21/31G01N33/6815G01N33/68B82Y40/00B82Y30/00
Inventor 吴红姣林鹏程刘骏区文滔王梓安
Owner GUANGDONG UNIV OF TECH
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