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Actinomycete strain SCAUT011 and application thereof

A technology of actinomycetes and strains, applied in the field of microorganisms, to achieve the effect of increasing production and promoting growth

Active Publication Date: 2022-02-08
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since A. glabra is a leguminous forage, previous studies mainly focused on the symbiotic relationship between rhizobia and A. glabra, but there is no report on the effect of rhizosphere growth-promoting bacteria on the growth of A. glabra.

Method used

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  • Actinomycete strain SCAUT011 and application thereof
  • Actinomycete strain SCAUT011 and application thereof
  • Actinomycete strain SCAUT011 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The separation and purification of embodiment 1 rhizosphere actinomycete SCAUT011

[0029] 1.1 Isolation of Actinomyces rhizobia from A. glabrata in Liangshan

[0030] In 2018, the purple vetch was collected in Huidong County, Liangshan Yi Autonomous Prefecture, Sichuan Province. The excess soil on the root system was shaken off, and the root was placed in a 50mL sterilized centrifuge tube filled with 25mL phosphate buffer solution, and treated with ultrasonic waves (150W) After 10 minutes, the roots were taken out under sterile conditions, and the supernatant was removed by centrifugation at 3000g for 10 minutes. The sediment was rhizosphere soil, and made 10 -1 、10 -2 , 10 -3 For gradient dilution, 100 ul of the suspension was uniformly spread on the modified Gowell's medium for separation, and each dilution concentration was repeated three times, and cultured in a constant temperature incubator at 28°C for 5-7 days. Pick the strains with typical actinomycete colon...

Embodiment 2

[0047] The growth-promoting function screening of embodiment 2 rhizosphere actinomycetes SCAUT011

[0048] 2.1 Screening of growth-promoting function

[0049] 2.1.1 Determination of IAA Production Ability

[0050] Take 20 μL of bacterial suspension and insert it into ISP containing 0.5mol / L tryptophan 4 In liquid culture medium, repeated 3 times, set blank control, cultured on a shaker at 28°C at 120r / min for 3d, centrifuged at 8000r / min for 24h, took 1mL of bacterial liquid supernatant, added 2mL of IAA chromogenic solution, reacted in dark at 25°C for 30min, 530nm Colorimetric at the wavelength and record the absorbance value. With the uninoculated liquid culture medium as the control zero adjustment, the indole acetic acid standard solution with the concentration of 0, 5, 20, 40, 60mg / l is the same as above to make the calibration, and calculate the concentration of IAA in the measurement solution.

[0051] 2.1.2 Determination of siderophore production capacity

[0052]...

Embodiment 3

[0084] Embodiment 3 hydroponic experiment of purple sweet potato

[0085] 3.1 Hydroponics experiment

[0086] The actinomycete strains to be tested were first activated on a TSA slant, and then transferred to TSA medium, cultivated overnight to the logarithmic phase, the growth of the strains was detected by the plate method, and the bacterial content was calculated.

[0087] After soaking the seeds of Viola glabrata overnight, carry out surface disinfection, use sterile tweezers to pick up the seeds and plant them in a petri dish with filter paper and sterile vermiculite, plant 10 seeds in each petri dish, and then add sterile water to keep the moisture , and maintain air circulation. Sterilize the tweezers, filter paper, petri dishes, plastic cups, vermiculite, quartz sand, and low-nitrogen nutrient solution required for the experiment.

[0088] Low nitrogen nutrient solution formula: KNO 3 10.1g, KH 2 PO 4 2.2g, MnSO 4 ·H 2 O 100.0mg, KCl 15.5g, ZnSO 4 ·7H 2 O 25...

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Abstract

The invention discloses an actinomycete strain Arthrobacter luteolus SCAUT011 and application thereof, wherein the actinomycete strain is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 61509. According to the invention, the Arthrobacter luteolus SCAUT011 provided through separating and screening has growth promoting capabilities of generating IAA and siderophores, dissolving phosphorus, degrading cellulose and chitin and the like, and also has stress resistance functions of salt resistance, acid and alkali resistance, drought resistance and the like, and is applied to liangshan Vicia villosa var .glabresens to obtain the effect of promoting the growth of the liangshan Vicia villosa var .glabresens; and the yield of forage grass is increased while the soil quality is improved, the tobacco planting soil is improved, and the actinomycete strain Arthrobacter luteolus SCAUT011 has good application potential in agricultural production.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to an actinomycete strain SCAUT011 and an application thereof. Background technique [0002] Liangshan Prefecture is one of the three major pastoral areas in Sichuan Province. Animal husbandry has a long history and is one of the main sources of cash income for farmers and herdsmen. Especially in high mountain areas, cash income from animal husbandry accounts for more than 70% of rural household income. Because Liangshan Prefecture has the climate characteristics of dry winter and spring, rainy summer, and distinct dryness and wetness, the problem of lack of green fodder for livestock during winter and spring is very prominent. However, in the mountainous areas around the basin of Liangshan Prefecture, especially in the Zhongshan and sub-alpine areas at an altitude of 1700-3200m, a large amount of land is left idle during winter and spring. Vicia villosa var.glabresens (Vi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/14A01N63/20A01P21/00C12R1/06C09K101/00
CPCC12N1/20C09K17/14A01N63/20C09K2101/00
Inventor 赵珂陈强李静邹立扣张紫薇辜运富廖德聪余秀梅向泉桔张凌子刘轶豪
Owner SICHUAN AGRI UNIV
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