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Manufacturing method of fruit fly salivary gland chromosome permanent loading slide

A production method and chromosome technology, applied in the preparation of test samples, measuring devices, sampling, etc., can solve the problems of not particularly stable operation, poor salivary gland extension, and the effect needs to be improved, so as to be easy to master and overcome the production of clothing pieces Unsatisfactory results, avoid poor stretching or even destroyed effects

Pending Publication Date: 2022-02-08
HUBEI POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In general, the prior art uses the permanent assembly of Drosophila salivary gland chromosomes obtained by knocking and / or pressing, but the presence of knocking and pressing can easily cause uneven stress on the salivary glands, salivary glands, etc. Glands are often poorly stretched and other defects, so not only the effect of the existing technology needs to be improved, but also the operation is not particularly stable

Method used

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  • Manufacturing method of fruit fly salivary gland chromosome permanent loading slide
  • Manufacturing method of fruit fly salivary gland chromosome permanent loading slide
  • Manufacturing method of fruit fly salivary gland chromosome permanent loading slide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] ① Obtain salivary glands: dissect the third instar larvae of Drosophila, obtain salivary gland 1 and remove the fat body; then clean up the sundries around salivary gland 1 on slide 2;

[0024] ②Staining: Routine staining of salivary gland 1;

[0025] ③Pressioning: cover the cover glass 3, place the two feet of the tweezers 4 on the cover glass 3 on both sides of the salivary gland 1, the angle a between the two feet of the tweezers 4 and the slide glass 2 is 30° , and then force downwards obliquely along the direction of the tweezers feet, and the direction of force is as follows figure 1 As indicated by the arrow, the cover glass 3 is in close contact with the slide glass 2 and at the same time slides horizontally relative to the slide glass 2. When the cover glass 3 is close to the slide glass 2 and the cover glass 3 When the excess dye solution below is squeezed out, stop using force, and pay attention to that the cover glass 3 should not slide relative to the slid...

Embodiment 2

[0028] ① Obtain salivary glands: dissect the third instar larvae of Drosophila, obtain salivary gland 1 and remove the fat body; then clean up the sundries around salivary gland 1 on slide 2;

[0029] ② Staining: Perform routine staining on salivary gland 1; after staining, remove excess dye solution, add 1 drop of Carnoy's fixative solution on salivary gland 1, and fix for 0.5 minutes;

[0030] ③Pressioning: cover the cover glass 3, place the two feet of the tweezers 4 on the cover glass 3 on both sides of the salivary gland 1, and the angle a between the two feet of the tweezers 4 and the slide glass 2 is 60° , and then force downwards obliquely along the direction of the tweezers feet, and the direction of force is as follows figure 1 As indicated by the arrow, the cover glass 3 is in close contact with the slide glass 2 and at the same time slides horizontally relative to the slide glass 2. When the cover glass 3 is close to the slide glass 2 and the cover glass 3 When th...

Embodiment 3

[0033] ① Obtain salivary glands: dissect the third instar larvae of Drosophila, obtain salivary gland 1 and remove the fat body; then clean up the sundries around salivary gland 1 on slide 2;

[0034] ② Staining: Perform routine staining on salivary gland 1; after staining, remove excess dye solution, add 2 drops of Carnoy's fixative solution on salivary gland 1, and fix for 2 minutes;

[0035] ③Pressioning: cover the cover glass 3, place the two feet of the tweezers 4 on the cover glass 3 on both sides of the salivary gland 1, and the angle a between the two feet of the tweezers 4 and the slide glass 2 is 45° , and then force downwards obliquely along the direction of the tweezers feet, and the direction of force is as follows figure 1 As indicated by the arrow, the cover glass 3 is in close contact with the slide glass 2 and at the same time slides horizontally relative to the slide glass 2. When the cover glass 3 is close to the slide glass 2 and the cover glass 3 When the...

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Abstract

The invention provides a preparation method of a fruit fly salivary gland chromosome permanent loading slide. The manufacturing method includes the steps of obtaining salivary glands, dyeing, tabletting in a direction inclined to a glass slide at an angle of 30-60 degrees, obtaining permanent loading and the like. The method is good in effect, the obtained drosophila salivary gland chromosome specimen is dispersed, and the dyeing result is good; the method is simple and easy to master; slide knocking or slide pressing is not needed, and the situation that salivary glands are unevenly stressed, and consequently stretching is poor or even damaged is avoided. In the operation process, the cover glass does not need to be tightly fixed, so that the defect of unsatisfactory loading and manufacturing results caused by slightly poor fixation of the cover glass is overcome, and the operation stability is high. If the salivary gland is treated for 0.5-3 minutes by using the Carnoy's stationary liquid after the dyeing is completed, the effect is better.

Description

technical field [0001] The invention relates to a method for manufacturing biological materials, in particular to a method for making permanent chromosomes of Drosophila salivary glands. Background technique [0002] The salivary gland cells in the larval stage of Drosophila and other Diptera insects are very large, and the salivary gland chromosomes have been replicated many times without being separated, resulting in each chromosome often having thousands of copies of chromosome filaments, so the salivary gland chromosomes are much larger than ordinary chromosomes many. After the salivary gland chromosomes are dyed, there will be horizontal stripes of different shades and density. The arrangement of these horizontal stripes means the arrangement of genes, representing the characteristics of insect species such as Drosophila; if there are deletions, duplications and inversions of chromosomes , translocation, etc., are easily identified on the salivary gland chromosome. Du...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/2813G01N1/30
Inventor 徐玲花
Owner HUBEI POLYTECHNIC UNIV
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