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Method for evaluating, detecting and/or predicting beef quality traits of consumption cattle

A technology for quality traits and beef, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve problems such as unreported research

Pending Publication Date: 2022-02-11
GUANGDONG OCEAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the relationship between ACOT12 gene and yak

Method used

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  • Method for evaluating, detecting and/or predicting beef quality traits of consumption cattle
  • Method for evaluating, detecting and/or predicting beef quality traits of consumption cattle
  • Method for evaluating, detecting and/or predicting beef quality traits of consumption cattle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 4

[0057] Example 1 Amplification of Sichuan Yak ACOT12 Gene SNP Site

[0058] 1. Experimental samples

Embodiment 1

[0059] The 102 Sichuan yaks in Example 1 came from a cattle farm in Aba Prefecture, Sichuan Province. The blood samples of healthy yaks in the same environment, the same feeding conditions, and the same period were collected and stored at -20°C for the extraction of blood genome DNA and the collection of related meat Quality trait data and analysis.

[0060] 2. Experimental method

[0061] 1. DNA extraction

[0062] According to the instructions, the blood genome DNA extraction kit was used to extract DNA from Sichuan yak blood samples, the concentration and purity of DNA were detected by micro-spectrophotometer, and the quality of DNA was detected by agarose gel electrophoresis.

[0063] 2. Primer design

[0064] According to the bovine ACOT12 gene sequence published in Ensembl (ENSBTAT00000014753.6), a pair of specific amplification primers was designed and synthesized by Sangon Bioengineering (Shanghai) Co., Ltd.

[0065] The sequences of the specific amplification prime...

Embodiment 2

[0080] Example 2 Population genetic characteristics of 3 SNPs loci of ACOT12 gene

[0081] 1. Experimental method

[0082] All 102 Sichuan yak sequencing results obtained in Example 1 were analyzed, using Popgene 32 software to calculate the gene frequency, genotype frequency, genetic heterozygosity (H), effective allele number (Ne) of alleles; PIC0.6 software calculates polymorphic information content (PIC).

[0083] 2. Experimental results

[0084] The population genetic characteristics of the three SNPs of the ACOT12 gene are shown in Table 1. It can be seen from Table 1 that the dominant genotype in SNP site 1 (E1-285T>C), SNP site 2 (E1-603 T>A), and SNP site 3 (E1-675 C>G) is TC , TA, CC, the dominant alleles are T, A, C, respectively, and the frequency is greater than 0.5.

[0085] The genetic heterozygosity (H) and polymorphic information content (PIC) of SNP locus 1 (E1-285 T>C) and SNP locus 2 (E1-603 T>A) were between 0.25 and 0.50, It shows that the above loci...

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Abstract

The invention discloses a method for evaluating, detecting and / or predicting beef quality traits of yaks, which is based on the combination of three molecular marker SNP sites related to the meat quality traits of yaks; wherein the three molecular marker SNP sites are respectively located at the 81354300th site, the 81353982th site and the 81353910th site of the 7th chromosome of the cattle genome of ARS-UCD 1.2 ver. It is further found that the genotype is significantly related to the pH 45 min value and the backfat thickness character, a method for evaluating, detecting and / or predicting the beef quality character of the cattle is established, a theoretical basis is provided for early breeding of high-quality beef cattle, and a foundation is laid for constructing core cattle herds with the high-quality beef quality character.

Description

technical field [0001] The invention relates to the technical field of yak breeding, in particular to a method for evaluating, detecting and / predicting the beef quality traits of yak consumption. Background technique [0002] The yak is a large ruminant with a tall body, thick coat, developed cardiopulmonary function, and strong stress resistance. It is the only rare breed of cattle that originated on the Qinghai-Tibet Plateau and has been bred in high-altitude areas to this day. It has excellent characteristics such as strong adaptability to high altitude areas, resistance to rough feeding, cold resistance and hard work. Compared with ordinary yellow beef, the protein content of yak meat is as high as 22.52%, the fat content is about 3.15%, and it is rich in mineral elements, and the proportion of amino acid structure is closer to that of the human body. As of 2019, the total number of yaks in the world is about 17 million, of which the number of yaks in China is more than...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 姜平赵志辉于海滨阮梦茹王浩铸隋亚民
Owner GUANGDONG OCEAN UNIVERSITY
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